Construct: ORF TRCN0000469779
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002275.1_s317c1
- Derived from:
- ccsbBroadEn_00702
- DNA Barcode:
- ATATGCGTGTAAAATATCACAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTM1 (2944)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469779
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | NM_146421.3 | 100% | 100% | |
| 2 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | NM_000561.4 | 83% | 83% | 455_565del |
| 3 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NM_147148.3 | 79.8% | 66% | (many diffs) |
| 4 | human | 2946 | GSTM2 | glutathione S-transferase mu 2 | NM_001142368.1 | 76.7% | 66.8% | (many diffs) |
| 5 | human | 2946 | GSTM2 | glutathione S-transferase mu 2 | NM_000848.4 | 76.4% | 71.1% | (many diffs) |
| 6 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NM_000850.5 | 76.4% | 70.6% | (many diffs) |
| 7 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | NM_000851.4 | 75.7% | 72.4% | (many diffs) |
| 8 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | XM_005270784.4 | 75.7% | 72.4% | (many diffs) |
| 9 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | XM_005270782.5 | 67.4% | 67.4% | 0_1ins102;353_463del |
| 10 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_024446577.1 | 67.2% | 62% | (many diffs) |
| 11 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001085.1 | 63.6% | 54.3% | (many diffs) |
| 12 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001087.2 | 54.4% | 44.7% | (many diffs) |
| 13 | human | 442245 | GSTM2P1 | glutathione S-transferase m... | NR_002932.2 | 43.1% | (many diffs) | |
| 14 | mouse | 14863 | Gstm2 | glutathione S-transferase, ... | NM_008183.3 | 71.7% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc catgatactg gggtactggg acatccgcgg gctggcccac gccatccgcc 121 tgcTCCTGGA ATACACAGAC TCAAGCTATG AGGAAAAGAA GTACACGATG GGGGACGCTC 181 CTGATTATGA CAGAAGCCAG TGGCTGAATG AAAAATTCAA GCTGGGCCTG GACTTTCCCA 241 ATCTGCCCTA CTTGATTGAT GGGGCTCACA AGATCACCCA GAGCAACGCC ATCTTGTGCT 301 ACATTGCCCG CAAGCACAAC CTGTGTGGGG AGACAGAAGA GGAGAAGATT CGTGTGGACA 361 TTTTGGAGAA CCAGACCATG GACAACCATA TGCAGCTGGG CATGATCTGC TACAATCCAG 421 AATTTGAGAA ACTGAAGCCA AAGTACTTGG AGGAACTCCC TGAAAAGCTA AAGCTCTACT 481 CAGAGTTTCT GGGGAAGCGG CCATGGTTTG CAGGAAACAA GGGCTTGGAG AAGATCTCTG 541 CCTACATGAA GTCCAGCCGC TTCCTCCCAA GACCTGTGTT CTCAAAGATG GCTGTCTGGG 601 GCAACAAGTA CTCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATATGC GTGTAAAATA TCACAGCAAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt