Transcript: Human NM_000851.4

Homo sapiens glutathione S-transferase mu 5 (GSTM5), mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
GSTM5 (2949)
Length:
1561
CDS:
63..719

Additional Resources:

NCBI RefSeq record:
NM_000851.4
NBCI Gene record:
GSTM5 (2949)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000851.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148649 CCTGCCATGTCTTGTCTTATT pLKO.1 1132 3UTR 100% 13.200 18.480 N GSTM5 n/a
2 TRCN0000414042 CTCAGGACTGTGCTCGAATTG pLKO_005 1170 3UTR 100% 10.800 8.640 N GSTM5 n/a
3 TRCN0000432767 CAGCTACATGGAACAGCAAAT pLKO_005 697 CDS 100% 10.800 7.560 N GSTM5 n/a
4 TRCN0000146884 CCTAAACTTGAAGGACTTCAT pLKO.1 596 CDS 100% 4.950 3.465 N GSTM5 n/a
5 TRCN0000149129 GAAGCGTATATTTGAGCCCAA pLKO.1 560 CDS 100% 2.160 1.512 N GSTM5 n/a
6 TRCN0000149670 GAACCAGGTTATGGATAACCA pLKO.1 365 CDS 100% 0.300 0.210 N GSTM5 n/a
7 TRCN0000147094 CCTTGACATGAAGCGTATATT pLKO.1 551 CDS 100% 15.000 9.000 N GSTM5 n/a
8 TRCN0000425811 ACTGTGCTATGACCCAGATTT pLKO_005 401 CDS 100% 13.200 7.920 N GSTM5 n/a
9 TRCN0000420215 AGAAGATTCGTGTGGACATTT pLKO_005 340 CDS 100% 13.200 6.600 Y GSTM1 n/a
10 TRCN0000147236 GAATACACAGACTCAAGCTAT pLKO.1 126 CDS 100% 4.950 2.475 Y GSTM5 n/a
11 TRCN0000252919 AGATCACCTTTGTGGATTTCA pLKO_005 517 CDS 100% 5.625 2.813 Y Gstm7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000851.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06334 pDONR223 100% 99.8% 99.5% None 199G>A n/a
2 ccsbBroad304_06334 pLX_304 0% 99.8% 99.5% V5 199G>A n/a
3 TRCN0000471453 CAGTGATATCGATAATGCGTACCT pLX_317 63.9% 99.8% 99.5% V5 199G>A n/a
4 ccsbBroadEn_06332 pDONR223 100% 87.4% 80.7% None (many diffs) n/a
5 ccsbBroad304_06332 pLX_304 0% 87.4% 80.7% V5 (many diffs) n/a
6 ccsbBroadEn_13866 pDONR223 100% 85.8% 82.1% None (many diffs) n/a
7 TRCN0000465604 TAACCACCCTTTTATTTACAACGA pLX_317 59.4% 85.8% 82.1% V5 (many diffs) n/a
8 ccsbBroadEn_00702 pDONR223 100% 75.7% 72.4% None (many diffs) n/a
9 ccsbBroad304_00702 pLX_304 0% 75.7% 72.4% V5 (many diffs) n/a
10 TRCN0000469779 ATATGCGTGTAAAATATCACAGCA pLX_317 88% 75.7% 72.4% V5 (many diffs) n/a
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