Construct: ORF TRCN0000469818

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018080.1_s317c1
Derived from:
ccsbBroadEn_12372
DNA Barcode:
AAGTAATACTGAATAGACTAACGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDZD4 (57595)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469818
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57595 PDZD4 PDZ domain containing 4 NM_001303512.1 100% 100%
2 human 57595 PDZD4 PDZ domain containing 4 NM_032512.4 99.2% 99.2% 296_297insGTACGTCCTCTCTGAGCT
3 human 57595 PDZD4 PDZ domain containing 4 NM_001303515.1 89.5% 89.5% 0_1ins243
4 human 57595 PDZD4 PDZ domain containing 4 NM_001303516.1 88.7% 88.7% 0_1ins243;53_54insGTACGTCCTCTCTGAGCT
5 human 57595 PDZD4 PDZ domain containing 4 NM_001303513.2 86.6% 86.4% 1_1delAins308;5_6delCC;8_9insT
6 human 57595 PDZD4 PDZ domain containing 4 NM_001303514.1 85.1% 85.1% 58_59ins345
7 mouse 245469 Pdzd4 PDZ domain containing 4 NM_001029868.2 86.5% 93% (many diffs)
8 mouse 245469 Pdzd4 PDZ domain containing 4 XM_006528017.3 83.9% 90.3% (many diffs)
9 mouse 245469 Pdzd4 PDZ domain containing 4 NM_001290540.1 72.7% 78.8% (many diffs)
10 mouse 245469 Pdzd4 PDZ domain containing 4 XM_006528020.2 72.2% 78.1% (many diffs)
11 mouse 245469 Pdzd4 PDZ domain containing 4 XM_006528021.3 68.3% 75% (many diffs)
12 mouse 245469 Pdzd4 PDZ domain containing 4 XM_006528022.3 66% 72.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2394
ORF length:
2325
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggatgtaat atgtgcgtgg tccagaaacc ggaggagcag tacaaagtga
121tgctgcaggt gaacgggaag gagctctcca agctgtctca ggagcaaact ctgcaggccc
181tgcgctcctc caaggagccc ctggtgatcc aggtgctgag acgcagcccc cgcctccggg
241gggacagctc ctgtcacgac ctgcagctgg tggacagtgg cactcagacc gacatcacct
301tcgagcatat catggcgctg ggcaagctgc gtccgcccac cccgcccatg gtcatcctgg
361agccgtacgt cctctctgag ctccccccaa tcagccatga gtattatgac ccggcggagt
421ttatggaggg cggcccgcag gaggcagacc gcttggatga gctggagtat gaggaggtgg
481agctgtataa aagcagccac cgggacaagc tgggcctgat ggtttgctac cgcacggacg
541acgaggagga cctgggcatt tatgtcggag aggtaaatcc caacagcatt gcagccaaag
601acggccggat ccgtgaggga gaccgcatca tccagattaa cggtgtagac gtccagaacc
661gggaagaggc ggtggccatc ctgagccagg aagagaacac caacatctcc ctgctggtgg
721cccgacctga gagtcagctg gcgaaaaggt ggaaggacag cgaccgggat gacttcctgg
781atgactttgg ctctgagaat gagggggagc tgcgtgctcg taaactgaaa tcaccccctg
841cccagcagcc cggaaacgaa gaggagaagg gggctcccga tgccggccca ggcctgagca
901acagccagga gctggacagc ggggtgggcc ggactgacga gagcacccgg aacgaagaga
961gctctgagca cgacctgctg ggggacgaac ccccgagctc caccaacacc ccgggaagcc
1021tgcgcaagtt tggcctgcaa ggggacgccc tgcagagccg ggacttccat ttcagcatgg
1081actctctgct ggccgagggg gcggggctgg gagggggcga cgtcccgggc ctcacggatg
1141aggagtatga gcgctaccgt gagctcctgg agatcaagtg ccacctggag aacggcaacc
1201agctgggcct cctctttccc cgggcctccg gaggcaacag cgccctggac gtcaaccgca
1261acgagagcct gggccacgag atggccatgc tggaggagga gctaaggcac ctggaattca
1321agtgccgcaa catactgcgg gcgcagaaga tgcagcagct gcgtgagcgc tgcatgaagg
1381cctggctgct ggaggaggag agcctctacg acctggcggc cagcgagccc aagaagcacg
1441agctgtccga catctccgag ctgcccgaga agtcggacaa ggacagcacc agcgcctaca
1501acactgggga gagctgccgc agcaccccgc tgcttgtgga gcccctgccc gagagccccc
1561tgcggcgggc catggccggc aactccaact tgaaccggac ccctcccggc cccgctgttg
1621ccacccccgc caaggcagct cctccaccgg ggagccccgc caagttccgg tccctctccc
1681gggatcctga ggccggccgg aggcagcacg cggaggagcg cggccgccgc aaccccaaga
1741cggggttgac cctggagcgt gtgggccctg aaagcagccc ttacctctcg cggcgccacc
1801gcggccaggg ccaggagggc gagcactacc acagctgcgt gcagctggcc ccgacgcgag
1861gcctggagga gctgggccac ggccccctga gcttggccgg tggcccTCGG GTGGGCGGGG
1921TGGCGGCCGC GGCCACTGAA GCACCGCGCA TGGAGTGGAA AGTGAAGGTG CGCAGCGACG
1981GAACCCGCTA CGTGGCCAAG CGGCCCGTGC GAGATCGGCT GCTGAAAGCC CGTGCCCTGA
2041AGATCCGGGA GGAGCGCAGC GGTATGACGA CCGACGACGA CGCGGTGAGC GAGATGAAGA
2101TGGGCCGCTA CTGGAGCAAG GAGGAGCGGA AGCAGCACCT GATCCGGGCC CGTGAGCAGC
2161GGAAGCGGCG CGAGTTCATG ATGCAGAGCC GGCTGGAGTG CCTGCGGGAG CAGCAGAATG
2221GCGACAGCAA GCCCGAGCTC AACATCATTG CCCTGAGCCA CCGCAAAACC ATGAAGAAGC
2281GGAACAAGAA GATCCTGGAC AACTGGATCA CCATCCAGGA GATGCTGGCC CACGGCGCGC
2341GCTCCGCCGA TGGCAAGCGG GTCTACAACC CTCTTCTCTC AGTCACCACC GTGTTGCCAA
2401CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
2461ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
2521TATCTTGTGG AAAGGACGAA AGTAATACTG AATAGACTAA CGCACGCGTT AAGTCgacaa
2581tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)

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