Construct: ORF TRCN0000469829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000999.1_s317c1
- Derived from:
- ccsbBroadEn_12742
- DNA Barcode:
- GACATGATCCGTATTAACAAGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COG3 (83548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83548 | COG3 | component of oligomeric gol... | XM_011535267.3 | 73.2% | 73.1% | (many diffs) |
| 2 | human | 83548 | COG3 | component of oligomeric gol... | NM_031431.4 | 53.4% | 53.3% | (many diffs) |
| 3 | human | 83548 | COG3 | component of oligomeric gol... | XR_001749695.2 | 31% | (many diffs) | |
| 4 | human | 83548 | COG3 | component of oligomeric gol... | XR_001749696.2 | 30.6% | (many diffs) | |
| 5 | human | 83548 | COG3 | component of oligomeric gol... | XR_429222.4 | 28.9% | (many diffs) | |
| 6 | human | 83548 | COG3 | component of oligomeric gol... | XM_011535266.2 | 26.4% | 26.3% | (many diffs) |
| 7 | mouse | 338337 | Cog3 | component of oligomeric gol... | XM_006519148.3 | 50% | 52.8% | (many diffs) |
| 8 | mouse | 338337 | Cog3 | component of oligomeric gol... | NM_177381.3 | 48% | 50.8% | (many diffs) |
| 9 | mouse | 338337 | Cog3 | component of oligomeric gol... | XM_011245092.1 | 23.7% | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaggcggcg ctgttgctgc tgcctgaggc ggcggcggag cgggacgcta 121 gggaaaagct ggctctctgg gatcggagac cggacacgac ggcgccgctg accgacaggc 181 agacggactc ggtattggag ctgaaggcgg cggcagagaa cttgccggtg ccagctgagc 241 ttccaattga agacttgtgc agtttaacat cccagtcact gcccattgaa ctgacttcag 301 tagtgcctga atctacagaa gacattctct tgaagggctt cacttcctta ggaatggaag 361 aagaaagaat tgaaaccgca cagcagtttt tctcatggtt tgcaaagctg caaactcaga 421 tggatcaaga tgaaggaact aaatatagac agatgaggga ttacttgtct gggtttcagg 481 agcagtgtga tgctatattg aatgatgtaa acagtgctct tcagcatctg gagtctttgc 541 agaaacagta tctttttgtg tccaataaga caggaaccct acatgaagcc tgtgaacagc 601 tcctaaaaga acagtcggaa cttgttgatc tggctgaaaa cattcaacaa aagctttcct 661 attttaacga attggaaact attaacacaa aattgaattc ccctacattg tcggtgaata 721 gtgacggatt tatacctatg ctggccaagt tagatgattg tataacatat atctcatctc 781 atcctaattt taaagattat cccatatatt tgctgaagtt taaacagtgt ctttctaaag 841 ctttgcacct catgaagaca tatactgtga acacactaca gaccctcaca agtcagttac 901 tgaaaaggga tccttcatct gtaccTAATG CAGACAATGC CTTCACATTA TTTTATGTGA 961 AATTTCGAGC TGCTGCCCCC AAAGTCAGAA CTCTTATTGA ACAAATAGAA CTGCGGTCTG 1021 AAAAAATACC TGAATACCAA CAACTGCTAA ATGATATCCA CCAGTGTTAC CTTGATCAGC 1081 GGGAGCTCCT TTTGGGCCCT AGTATTGCTT GCACTGTTGC AGAGTTAACC AGCCAAAATA 1141 ATAGAGATCA CTGTGCCTTG GTTCGTAGTG GCTGTGCCTT CATGGTTCAT GTCTGCCAGG 1201 ATGAACACCA ACTTTACAAT GAATTTTTCA CAAAACCAAC ATCAAAATTA GATGAGCTTT 1261 TGGAGAAACT GTGTGTGTCA TTGTATGATG TCTTCAGGCC ATTGATCATT CATGTTATTC 1321 ACTTAGAGAC TCTGTCGGAA CTTTGTGGGA TTCTTAAAAA TGAAGTGCTT GAAGATCATG 1381 TGCAGAACAA TGGTAAATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGACATGA TCCGTATTAA 1561 CAAGGGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt