Construct: ORF TRCN0000469977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006900.1_s317c1
- Derived from:
- ccsbBroadEn_04281
- DNA Barcode:
- AAGTACGCAATTACATCCAACGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCM2 (83605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_031443.3 | 100% | 100% | |
| 2 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001029835.2 | 95.1% | 92.7% | (many diffs) |
| 3 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001363458.2 | 91.5% | 91.5% | 745_867del |
| 4 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_006715785.4 | 88.5% | 86% | (many diffs) |
| 5 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_011515561.2 | 87.4% | 85.3% | (many diffs) |
| 6 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001167934.1 | 86.9% | 86.9% | 27_28ins174 |
| 7 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_011515563.3 | 81.1% | 78.7% | (many diffs) |
| 8 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001363459.2 | 79.5% | 79.5% | 27_28ins174;571_693del |
| 9 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001167935.1 | 79.5% | 79.5% | 471_472ins273 |
| 10 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_006715786.3 | 75.6% | 73.4% | (many diffs) |
| 11 | human | 83605 | CCM2 | CCM2 scaffold protein | NR_030770.2 | 72.7% | (many diffs) | |
| 12 | human | 83605 | CCM2 | CCM2 scaffold protein | XR_428088.3 | 69.5% | (many diffs) | |
| 13 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_017012671.1 | 59.4% | 50.1% | (many diffs) |
| 14 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_017012672.2 | 51.6% | 41.1% | (many diffs) |
| 15 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_017012673.1 | 49.6% | 41.9% | (many diffs) |
| 16 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | NM_146014.3 | 88.1% | 92.7% | (many diffs) |
| 17 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | XM_006514622.1 | 86.8% | 91.1% | (many diffs) |
| 18 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | NM_001190343.1 | 75.7% | 80.1% | (many diffs) |
| 19 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | NM_001190344.1 | 74.3% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaggagggc aagaagggca agaagcctgg aattgtctcg ccatttaaac 121 gagtattcct aaaaggtgaa aagagtagag ataagaaagc ccatgagaag gtgacagaga 181 ggcgccctct gcacactgtg gtgttgtcat tgcctgagcg cgtcgagcca gacagactgc 241 tgagcgacta tattgagaag gaggtaaagt atttaggtca gttaacgtcc ataccaggat 301 acctgaatcc ctccagtagg actgaaatcc tgcatttcat agacaatgca aagagagccc 361 accagcttcc gggacacttg actcaggagc acgatgctgt gctcagcctg tctgcgtaca 421 acgtcaagct ggcctggagg gacggggagg atatcatcct cagggtgccc atccatgaca 481 tcgccgccgt ctcctatgtt cgggatgacg ctgcacacct ggtggtcctg aagacagccc 541 aggacccagg gatctccccc agccagagtc tgtgtgcgga aagttccaga ggcctcagtg 601 caggctccct gtcggagagt gcagttgggc ccgtggaggc atgctgcctg gtcatcctgg 661 ctgcagagag caaggtcgct gcggaggagc tttgctgtct gctaggccag gtcttccagg 721 ttgtttacac ggagtccacc atcgactttc tggacagagc gatatttgat ggggcctcta 781 ccccgaccca ccacctgtcc ctgcacagcg atgactcttc tacaaaagtg gacattaagg 841 agacctacga ggtggaagcc agcactttct gcttccctga atctgtggat gtgggtggtg 901 catcacccca cagcaagacc atcagtgaga gcgagctgag cgccagcgcc actgagctgc 961 tgcaggacta catgctgacg ctgcgcacca agctgtcatc acaggagatc cagcagtttg 1021 cagcactgct gcacgagtac cgcaatgggg cctctatcca cgagttcTGC ATCAACCTGC 1081 GGCAGCTCTA CGGGGACAGC CGCAAGTTCC TGCTGCTTGG TCTGAGGCCC TTCATCCCTG 1141 AGAAGGACAG CCAGCACTTC GAGAACTTCC TGGAGACCAT TGGCGTGAAG GATGGCCGCG 1201 GCATCATCAC TGACAGCTTT GGCAGGCACC GGCGGGCCCT GAGCACCACA TCCAGTTCCA 1261 CCACCAATGG GAACAGGGCC ACGGGCAGCT CTGATGACCG GTCGGCACCC TCAGAGGGGG 1321 ATGAGTGGGA CCGCATGATC TCGGACATCA GCAGCGACAT TGAGGCGCTG GGCTGCAGCA 1381 TGGACCAGGA CTCAGCATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAGTACG CAATTACATC 1561 CAACGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt