Construct: ORF TRCN0000470036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012563.1_s317c1
- Derived from:
- ccsbBroadEn_02025
- DNA Barcode:
- AGGTAGGCTCTCAACGCTGCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NRP2 (8828)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470036
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8828 | NRP2 | neuropilin 2 | NM_003872.3 | 99.9% | 100% | 1404T>C |
| 2 | human | 8828 | NRP2 | neuropilin 2 | NM_201266.2 | 99.4% | 99.4% | 1404T>C;2424_2438del |
| 3 | human | 8828 | NRP2 | neuropilin 2 | NM_201279.2 | 98.1% | 98.1% | 1404T>C;2424_2425ins51 |
| 4 | human | 8828 | NRP2 | neuropilin 2 | NM_018534.4 | 91.2% | 87.9% | (many diffs) |
| 5 | human | 8828 | NRP2 | neuropilin 2 | NM_201267.2 | 90.8% | 88.4% | (many diffs) |
| 6 | human | 8828 | NRP2 | neuropilin 2 | XM_017005188.2 | 89.1% | 88% | (many diffs) |
| 7 | human | 8828 | NRP2 | neuropilin 2 | XM_017005187.2 | 88.6% | 87.5% | (many diffs) |
| 8 | human | 8828 | NRP2 | neuropilin 2 | XR_923056.3 | 78.6% | (many diffs) | |
| 9 | human | 8828 | NRP2 | neuropilin 2 | XR_923057.3 | 77% | (many diffs) | |
| 10 | human | 8828 | NRP2 | neuropilin 2 | NM_201264.1 | 59.4% | 59.5% | (many diffs) |
| 11 | human | 8828 | NRP2 | neuropilin 2 | XR_923055.3 | 42.5% | (many diffs) | |
| 12 | mouse | 18187 | Nrp2 | neuropilin 2 | NM_001077404.1 | 89% | 94.6% | (many diffs) |
| 13 | mouse | 18187 | Nrp2 | neuropilin 2 | NM_001077403.1 | 88.5% | 94% | (many diffs) |
| 14 | mouse | 18187 | Nrp2 | neuropilin 2 | NM_010939.2 | 87.8% | 93% | (many diffs) |
| 15 | mouse | 18187 | Nrp2 | neuropilin 2 | NM_001077405.1 | 87.5% | 93% | (many diffs) |
| 16 | mouse | 18187 | Nrp2 | neuropilin 2 | NM_001077406.1 | 81.2% | 83.7% | (many diffs) |
| 17 | mouse | 18187 | Nrp2 | neuropilin 2 | NM_001077407.1 | 81.2% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2847
- ORF length:
- 2778
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatatgttt cctctcacct gggttttctt agccctctac ttttcaagac 121 accaagtgag aggccaacca gacccaccgt gcggaggtcg tttgaattcc aaagatgctg 181 gctatatcac ctctcccggt tacccccagg actacccctc ccaccagaac tgcgagtgga 241 ttgtttacgc ccccgaaccc aaccagaaga ttgtcctcaa cttcaaccct cactttgaaa 301 tcgagaagca cgactgcaag tatgacttta tcgagattcg ggatggggac agtgaatccg 361 cagacctcct gggcaaacac tgtgggaaca tcgccccgcc caccatcatc tcctcgggct 421 ccatgctcta catcaagttc acctccgact acgcccggca gggggcaggc ttctctctgc 481 gctacgagat cttcaagaca ggctctgaag attgctcaaa aaacttcaca agccccaacg 541 ggaccatcga atctcctggg tttcctgaga agtatccaca caacttggac tgcaccttta 601 ccatcctggc caaacccaag atggagatca tcctgcagtt cctgatcttt gacctggagc 661 atgacccttt gcaggtggga gagggggact gcaagtacga ttggctggac atctgggatg 721 gcattccaca tgttggcccc ctgattggca agtactgtgg gaccaaaaca ccctctgaac 781 ttcgttcatc gacggggatc ctctccctga cctttcacac ggacatggcg gtggccaagg 841 atggcttctc tgcgcgttac tacctggtcc accaagagcc actagagaac tttcagtgca 901 atgttcctct gggcatggag tctggccgga ttgctaatga acagatcagt gcctcatcta 961 cctactctga tgggaggtgg acccctcaac aaagccggct ccatggtgat gacaatggct 1021 ggacccccaa cttggattcc aacaaggagt atctccaggt ggacctgcgc tttttaacca 1081 tgctcacggc catcgcaaca cagggagcga tttccaggga aacacagaat ggctactatg 1141 tcaaatccta caagctggaa gtcagcacta atggagagga ctggatggtg taccggcatg 1201 gcaaaaacca caaggtattt caagccaaca acgatgcaac tgaggtggtt ctgaacaagc 1261 tccacgctcc actgctgaca aggtttgtta gaatccgccc tcagacctgg cactcaggta 1321 tcgccctccg gctggagctc ttcggctgcc gggtcacaga tgctccctgc tccaacatgc 1381 tggggatgct ctcaggcctc attgcagact cccagatctc cgcctcttcc acccaggaat 1441 acctctggag ccccagtgca gcccgcctgg tcagcagccg ctcgggctgg ttccctcgaa 1501 tccctcaggc ccagcccggt gaggagtggc ttcaggtaga tctgggaaca cccaagacag 1561 tgaaaggtgt catcatccag ggagcccgcg gaggagacag tatcactgct gtggaagcca 1621 gagcatttgt gcgcaagttc aaagtctcct acagcctaaa cggcaaggac tgggaataca 1681 ttcaggaccc caggacccag cagccaaagc tgttcgaagg gaacatgcac tatgacaccc 1741 ctgacatccg aaggtttgac cccattccgg cacagtatgt gcgggtatac ccggagaggt 1801 ggtcgccggc ggggattggg atgcggctgg aggtgctggg ctgtgactgg acagactcca 1861 agcccacggt agagacgctg ggacccactg tgaagagcga agagacaacc accccctacc 1921 ccaccgaaga ggaggccaca gagtgtgggg agaactgcag ctttgaggat gacaaagatt 1981 tgcagctccc ttcgggattc aattgcaact tcgatttcct cgaggagccc tgtggttgga 2041 tgtatgacca tgccaagtgg ctccggacca cctgggccag cagctccagc ccaaacgacc 2101 ggacgtttcc agatgacagg aatttcttgc ggctgcagag tgacagccag agagagggcc 2161 agtatgcccg gctcatcagc ccccctgtcc acctgccccg aagcccggtg tgcatggagt 2221 tccagtacca ggccacgggc ggccgcgggg tggcgctgca ggtggtgcgg gaagccagcc 2281 aggagagcaa gttgctgtgg gtcatccgtg aggaccaggg cggcgagtgg aagcacgggc 2341 ggatcatcct gcccagctac gacatggagt accagattgt gttcgaggGA GTGATAGGGA 2401 AAGGACGTTC CGGAGAGATT GCCATTGATG ACATTCGGAT AAGCACTGAT GTCCCACTGG 2461 AGAACTGCAT GGAACCCATC TCGGCTTTTG CAGTGGACAT CCCAGAAATA CATGAGAGAG 2521 AAGGATATGA AGATGAAATT GATGATGAAT ACGAGGTGGA CTGGAGCAAT TCTTCTTCTG 2581 CAACCTCAGG GTCTGGCGCC CCCTCGACCG ACAAAGAAAA GAGCTGGCTG TACACCCTGG 2641 ATCCCATCCT CATCACCATC ATCGCCATGA GCTCACTGGG CGTCCTCCTG GGGGCCACCT 2701 GTGCAGGCCT CCTGCTCTAC TGCACCTGTT CCTACTCGGG CCTGAGCTCC CGAAGCTGCA 2761 CCACACTGGA GAACTACAAC TTCGAGCTCT ACGATGGCCT TAAGCACAAG GTCAAGATGA 2821 ACCACCAAAA GTGCTGCTCC GAGGCATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2881 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2941 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGGTAGGC 3001 TCTCAACGCT GCCTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3061 aagatt