Construct: ORF TRCN0000470051
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011051.1_s317c1
- Derived from:
- ccsbBroadEn_00094
- DNA Barcode:
- AGACATCCAACAGCAAGTTGGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARF1 (375)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470051
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001024226.1 | 100% | 100% | |
| 2 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001024227.1 | 100% | 100% | |
| 3 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001024228.2 | 100% | 100% | |
| 4 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001658.4 | 100% | 100% | |
| 5 | human | 377 | ARF3 | ADP ribosylation factor 3 | NM_001659.3 | 83.7% | 96.1% | (many diffs) |
| 6 | human | 377 | ARF3 | ADP ribosylation factor 3 | XM_005268856.1 | 83.7% | 96.1% | (many diffs) |
| 7 | human | 377 | ARF3 | ADP ribosylation factor 3 | XM_024448972.1 | 83.7% | 96.1% | (many diffs) |
| 8 | human | 375 | ARF1 | ADP ribosylation factor 1 | XM_017001233.1 | 73.6% | 64.3% | 382_517del;636_637ins43 |
| 9 | human | 377 | ARF3 | ADP ribosylation factor 3 | XM_006719391.4 | 63.1% | 68.5% | (many diffs) |
| 10 | mouse | 11840 | Arf1 | ADP-ribosylation factor 1 | NM_001130408.1 | 91.8% | 100% | (many diffs) |
| 11 | mouse | 11840 | Arf1 | ADP-ribosylation factor 1 | NM_007476.3 | 91.8% | 100% | (many diffs) |
| 12 | mouse | 11842 | Arf3 | ADP-ribosylation factor 3 | NM_007478.3 | 84.1% | 96.1% | (many diffs) |
| 13 | mouse | 11842 | Arf3 | ADP-ribosylation factor 3 | XM_011245445.1 | 84.1% | 96.1% | (many diffs) |
| 14 | mouse | 102632770 | LOC102632770 | ADP-ribosylation factor 1 p... | XR_373388.3 | 27.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaacatcttc gccaacctct tcaagggcct ttttggcaaa aaagaaatgc 121 gcatcctcat ggtgggcctg gatgctgcag ggaagaccac gatcctctac aagcttaagc 181 tggGTGAGAT CGTGACCACC ATTCCCACCA TAGGCTTCAA CGTGGAAACC GTGGAGTACA 241 AGAACATCAG CTTCACTGTG TGGGACGTGG GTGGCCAGGA CAAGATCCGG CCCCTGTGGC 301 GCCACTACTT CCAGAACACA CAAGGCCTGA TCTTCGTGGT GGACAGCAAT GACAGAGAGC 361 GTGTGAACGA GGCCCGTGAG GAGCTCATGA GGATGCTGGC CGAGGACGAG CTCCGGGATG 421 CTGTCCTCCT GGTGTTCGCC AACAAGCAGG ACCTCCCCAA CGCCATGAAT GCGGCCGAGA 481 TCACAGACAA GCTGGGGCTG CACTCACTAC GCCACAGGAA CTGGTACATT CAGGCCACCT 541 GCGCCACCAG CGGCGACGGG CTCTATGAAG GACTGGACTG GCTGTCCAAT CAGCTCCGGA 601 ACCAGAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGACAT CCAACAGCAA GTTGGGTGAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt