Construct: ORF TRCN0000470116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009976.1_s317c1
- Derived from:
- ccsbBroadEn_07186
- DNA Barcode:
- TCTGCCCACCCAGCGGGCCTGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA4 (7903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7903 | ST8SIA4 | ST8 alpha-N-acetyl-neuramin... | NM_175052.3 | 99.8% | 99.4% | 20G>A |
| 2 | human | 7903 | ST8SIA4 | ST8 alpha-N-acetyl-neuramin... | XM_011543630.2 | 64.1% | 63.6% | 20G>A;504_783delinsG |
| 3 | human | 7903 | ST8SIA4 | ST8 alpha-N-acetyl-neuramin... | XM_005272078.3 | 59.4% | 59.2% | 20G>A;505_846del |
| 4 | human | 7903 | ST8SIA4 | ST8 alpha-N-acetyl-neuramin... | NM_005668.6 | 46.7% | 46.5% | 20G>A;505_1077del |
| 5 | mouse | 20452 | St8sia4 | ST8 alpha-N-acetyl-neuramin... | NM_001159745.1 | 56.8% | 60.5% | (many diffs) |
| 6 | mouse | 20452 | St8sia4 | ST8 alpha-N-acetyl-neuramin... | XM_006529275.3 | 42.4% | 45.2% | (many diffs) |
| 7 | mouse | 20452 | St8sia4 | ST8 alpha-N-acetyl-neuramin... | NM_009183.2 | 42.1% | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ctccattagg aagaagtgga cgatctgcac aataagtctg ctcctgatct 121 tttataagac aaaagaaata gcaagaactg aggagcacca ggagacgcaa ctcatcggag 181 atggtgaatt gtctttgagt cggtcacttg tcaatagctc tgataaaatc attcgaaagg 241 ctggctcttc aatcttccag cacaatgtag aaggttggaa aatcaattcc tctttggtcc 301 tagagataag gaagaacata cttcgtttct tagatgcaga acgagatgtg tcagtggtca 361 agagcagttt taagcctggt gatgtcatac actatgtgct tgacaggcgc cggacactaa 421 acatttctca tgatctacat agcctcctac ctgaagtttc accaatgaag aatcgcaggt 481 ttaagacctg tgcagttgtt ggaaattctg gcattctgtt agacagtgaa tgtggaaagg 541 agattgacag tcacaatttt GTAATAAGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCTGC 721 CCACCCAGCG GGCCTGAGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt