Transcript: Mouse XM_006517570.3

PREDICTED: Mus musculus phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pik3r1 (18708)
Length:
5836
CDS:
506..1717

Additional Resources:

NCBI RefSeq record:
XM_006517570.3
NBCI Gene record:
Pik3r1 (18708)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006517570.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304742 AGTCAAGCATTGCGTCATTAA pLKO_005 1540 CDS 100% 13.200 18.480 N Pik3r1 n/a
2 TRCN0000304741 GTACGAGACGCATCTACTAAA pLKO_005 611 CDS 100% 13.200 18.480 N Pik3r1 n/a
3 TRCN0000304740 CCAGACGTTTCTAACCTTATC pLKO_005 1905 3UTR 100% 10.800 15.120 N Pik3r1 n/a
4 TRCN0000068197 CGGCGATTACACTCTTACACT pLKO.1 637 CDS 100% 3.000 4.200 N Pik3r1 n/a
5 TRCN0000302387 CGGCGATTACACTCTTACACT pLKO_005 637 CDS 100% 3.000 4.200 N Pik3r1 n/a
6 TRCN0000068195 GTCGGGAATATGATAGATTAT pLKO.1 921 CDS 100% 13.200 10.560 N Pik3r1 n/a
7 TRCN0000068196 CAACCGAAACAAAGCGGAGAA pLKO.1 1429 CDS 100% 4.050 3.240 N Pik3r1 n/a
8 TRCN0000302323 CAACCGAAACAAAGCGGAGAA pLKO_005 1429 CDS 100% 4.050 3.240 N Pik3r1 n/a
9 TRCN0000039903 GCGCTATGCAATTCTTAATTT pLKO.1 2563 3UTR 100% 15.000 10.500 N PIK3R1 n/a
10 TRCN0000039906 CGGTACAGCAAAGAATACATA pLKO.1 1049 CDS 100% 5.625 3.938 N PIK3R1 n/a
11 TRCN0000033288 GCGGTACAGCAAAGAATACAT pLKO.1 1048 CDS 100% 5.625 3.938 N PIK3R1 n/a
12 TRCN0000068194 CCTCAATGTCACACTAGCATA pLKO.1 1669 CDS 100% 4.950 3.465 N Pik3r1 n/a
13 TRCN0000068193 CGAAGCATTTAATGAAACCAT pLKO.1 994 CDS 100% 3.000 2.100 N Pik3r1 n/a
14 TRCN0000430557 TAACCATGGTGCTTGTTAATG pLKO_005 2082 3UTR 100% 13.200 18.480 N PIK3R1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006517570.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06728 pDONR223 100% 79.9% 88.1% None (many diffs) n/a
2 ccsbBroad304_06728 pLX_304 47% 79.9% 88.1% V5 (many diffs) n/a
3 TRCN0000466732 GGCTCTGGTGCGGACAGCAGAAGC pLX_317 34.3% 79.9% 88.1% V5 (many diffs) n/a
4 ccsbBroadEn_14763 pDONR223 0% 79.9% 88.1% None (many diffs) n/a
5 ccsbBroad304_14763 pLX_304 49.6% 79.9% 88.1% V5 (many diffs) n/a
6 TRCN0000470397 TTCACCGGAGCCTGAGATAGGCTA pLX_317 32.4% 79.9% 88.1% V5 (many diffs) n/a
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