Construct: ORF TRCN0000470421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010870.1_s317c1
- Derived from:
- ccsbBroadEn_06754
- DNA Barcode:
- CTCTATGCCAAACTACCAATACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPARD (5467)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470421
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5467 | PPARD | peroxisome proliferator act... | NM_177435.3 | 99.9% | 100% | 489C>T |
| 2 | human | 5467 | PPARD | peroxisome proliferator act... | XM_024446474.1 | 98.7% | 98.3% | (many diffs) |
| 3 | human | 5467 | PPARD | peroxisome proliferator act... | NM_001171818.2 | 81.6% | 81.4% | (many diffs) |
| 4 | human | 5467 | PPARD | peroxisome proliferator act... | NM_006238.5 | 81.6% | 81.4% | (many diffs) |
| 5 | human | 5467 | PPARD | peroxisome proliferator act... | XM_005249193.1 | 81.6% | 81.4% | (many diffs) |
| 6 | human | 5467 | PPARD | peroxisome proliferator act... | XM_006715120.1 | 81.6% | 81.4% | (many diffs) |
| 7 | human | 5467 | PPARD | peroxisome proliferator act... | XM_006715123.1 | 81.6% | 81.4% | (many diffs) |
| 8 | human | 5467 | PPARD | peroxisome proliferator act... | XM_011514707.1 | 81.6% | 81.4% | (many diffs) |
| 9 | human | 5467 | PPARD | peroxisome proliferator act... | XM_011514710.1 | 81.6% | 81.4% | (many diffs) |
| 10 | human | 5467 | PPARD | peroxisome proliferator act... | XM_017010972.1 | 81.6% | 81.4% | (many diffs) |
| 11 | human | 5467 | PPARD | peroxisome proliferator act... | XM_017010973.1 | 81.6% | 81.4% | (many diffs) |
| 12 | human | 5467 | PPARD | peroxisome proliferator act... | XM_017010974.1 | 81.6% | 81.4% | (many diffs) |
| 13 | human | 5467 | PPARD | peroxisome proliferator act... | NM_001171819.1 | 72.6% | 71.6% | (many diffs) |
| 14 | human | 5467 | PPARD | peroxisome proliferator act... | NM_001171820.1 | 59.4% | 59.1% | (many diffs) |
| 15 | mouse | 19015 | Ppard | peroxisome proliferator act... | NM_011145.3 | 73% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcagccacag gaggaagccc ctgaggtccg ggaagaggag gagaaagagg 121 aagtggcaga ggcagaagga gccccagagc tcaatggggg accacagcat gcacttcctt 181 ccagcagcta cacagacctc tcccggagct cctcgccacc ctcactgctg gaccaactgc 241 agatgggctg tgacggggcc tcatgcggca gcctcaacat ggagtgccgg gtgtgcgggg 301 acaaggcatc gggcttccac tacggtgttc atgcatgtga ggggtgcaag ggcttcttcc 361 gtcgtacgat ccgcatgaag ctggagtacg agaagtgtga gcgcagctgc aagattcaga 421 agaagaaccg caacaagtgc cagtactgcc gcttccagaa gtgcctggca ctgggcatgt 481 cacacaacgc tatccgtttt ggtcggatgc cggaggctga gaagaggaag ctggtggcag 541 ggctgactgc aaatgagggg agccagtaca acccacaggt ggccgacctg aaggccttct 601 ccaagcacat ctacaatgcc tacctgaaaa acttcaacat gaccaaaaag aaggcccgca 661 gcatcctcac cggcaaagcc agccacacgg cgccctttgt gatccacgac atcgagacat 721 tgtggcaggc agagaagggg ctggtgtgga agcagttggt gaatggcctg ccTCCCTACA 781 AGGAGATCAG CGTGCACGTC TTCTACCGCT GCCAGTGCAC CACAGTGGAG ACCGTGCGGG 841 AGCTCACTGA GTTCGCCAAG AGCATCCCCA GCTTCAGCAG CCTCTTCCTC AACGACCAGG 901 TTACCCTTCT CAAGTATGGC GTGCACGAGG CCATCTTCGC CATGCTGGCC TCTATCGTCA 961 ACAAGGACGG GCTGCTGGTA GCCAACGGCA GTGGCTTTGT CACCCGTGAG TTCCTGCGCA 1021 GCCTCCGCAA ACCCTTCAGT GATATCATTG AGCCTAAGTT TGAATTTGCT GTCAAGTTCA 1081 ACGCCCTGGA ACTTGATGAC AGTGACCTGG CCCTATTCAT TGCGGCCATC ATTCTGTGTG 1141 GAGGTGAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCTAT GCCAAACTAC CAATACATAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt