Construct: ORF TRCN0000470451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011162.1_s317c1
- Derived from:
- ccsbBroadEn_02962
- DNA Barcode:
- AATGGACCGCACAGCGCCGTTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INTS6 (26512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470451
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26512 | INTS6 | integrator complex subunit 6 | NM_012141.3 | 100% | 100% | |
| 2 | human | 26512 | INTS6 | integrator complex subunit 6 | XM_011535040.3 | 100% | 100% | |
| 3 | human | 26512 | INTS6 | integrator complex subunit 6 | NM_001039937.2 | 98.5% | 98.5% | 0_1ins39 |
| 4 | human | 26512 | INTS6 | integrator complex subunit 6 | NM_001306091.2 | 79.9% | 79.9% | 0_1ins534 |
| 5 | human | 26512 | INTS6 | integrator complex subunit 6 | XM_011535041.2 | 75.8% | 75.8% | 0_1ins642 |
| 6 | human | 26512 | INTS6 | integrator complex subunit 6 | XM_017020540.2 | 51.2% | 51.2% | 0_1ins1296 |
| 7 | human | 26512 | INTS6 | integrator complex subunit 6 | NM_001039938.2 | 12.8% | 12.8% | 341T>G;343G>A;345_345delTins2317 |
| 8 | mouse | 18130 | Ints6 | integrator complex subunit 6 | NM_008715.2 | 92.3% | 95.9% | (many diffs) |
| 9 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_011244986.2 | 92.3% | 95.9% | (many diffs) |
| 10 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_006518680.3 | 92.2% | 95.8% | (many diffs) |
| 11 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_011244985.2 | 92.2% | 95.8% | (many diffs) |
| 12 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_017315908.1 | 92.2% | 95.8% | (many diffs) |
| 13 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_006518682.3 | 91.2% | 94.8% | (many diffs) |
| 14 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_017315909.1 | 91.2% | 94.8% | (many diffs) |
| 15 | mouse | 18130 | Ints6 | integrator complex subunit 6 | XM_006518683.2 | 72.8% | 75.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2727
- ORF length:
- 2661
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc catcttactg ttcctgatag acacgtctgc ctctatgaac cagcgcagcc 121 atctgggcac cacctacctg gacacggcca aaggcgcggt agagaccttc atgaagctcc 181 gtgcccggga ccctgccagc agaggagaca ggtatatgct ggtcactttc gaagagccgc 241 cctatgctat caaggctgga tggaaagaaa accatgcaac gtttatgaat gaattgaaaa 301 accttcaggc tgaaggactt acgactcttg gccaatccct aaggacagct tttgatttat 361 taaatttaaa tagattagta actggcatag acaactatgg gcagggaaga aacccttttt 421 tcttggagcc agcaataatt atcacaatta ctgatgggag caagttgact accaccagtg 481 gagtccagga tgagcttcat ttacctctta attctccttt gcctggaagt gaattgacca 541 aggaaccttt tcgttgggat cagagactct ttgcattagt gttgcggttg cctggcacca 601 tgtcagtaga atcagaacag ttgacaggtg tgcctttaga tgactctgca atcacaccaa 661 tgtgtgaagt gacaggcggc cgttcatatt ctgtgtgttc tccaagaatg cttaatcagt 721 gtctggagtc cttggtgcag aaagtacaaa gtggggtggt aataaacttt gaaaaagcag 781 gaccagatcc ttcccctgta gaagatgggc agccagatat atcaaggcct tttggatctc 841 agccttggca tagctgtcac aaactcatat atgtcagacc aaatcctaaa actggggttc 901 ctataggtca ttggcctgtt ccagagtctt tttggccaga tcaaaattcg ccaacactac 961 cacctcgtac atctcatcct gtagtgaagt tttcctgtac agactgtgaa ccaatggtta 1021 ttgataaact tccttttgac aaatatgagt tggaaccttc accactgact caatttatcc 1081 tggaaaggaa atctcctcaa acatgttggc aggtgtacgt gagcaatagt gcaaaataca 1141 gtgaacttgg tcatcctttt ggttacttga aagccagtac agcactgaac tgtgtcaact 1201 tatttgtgat gccttacaat tatccagtcc ttcttcccct cttagatgac ttgtttaaag 1261 tgcataaagc aaaaccaaca ttgaagtgga gacagtcatt tgaaagttat ttgaagacaa 1321 tgcctcccta ctatcttggg cccttgaaga aagctgttag gatgatggga gcacctaacc 1381 taatagcaga cagtatggaa tatggactta gttacagtgt catttcatac ctcaaaaaac 1441 tgagtcaaca ggccaaaata gaatctgatc gagtcattgg atctgtaggc aaaaaagtag 1501 tacaggagac tggaataaaa gtccggagcc gatcacatgg tttatcaatg gcatatagga 1561 aagattttca acaactcctc cagggaattt cagaggatgt ccctcacaga ctgctagacc 1621 ttaatatgaa ggaatacact gggttccaag ttgctttgct gaataaggat ttgaagccac 1681 agacatttag aaatgcttat gacataccaa gacgaaatct tttggatcac ttaacaagaa 1741 tgagatctaa tcttttgaag agcactcgca gatttctgaa aggacaggac gaagatcaag 1801 tgcacagtgt tcctatagca caaatgggga actaccagga atacctcaag caagtacctt 1861 ctccactaag agaacttgat cctgatcagc cacgaaggtt gcatacattt ggcaacccct 1921 ttaagctgga taagaagggt atgatgatag atgaagcaga tgaatttgtg gctggacctc 1981 aaaataaaca taaacgaccc ggagaaccaa atatgcaagg gatccctaaa agacgtcggt 2041 gtatgtctcc actactaaga ggcagacagc agaatcctgt tgtaaacaat catattgggg 2101 gaaaaggacc acctgcacct acaactcaag cacagccaga tcttattaaa cctcttcctc 2161 ttcataaaat ttcagaaacc actaatgatt cgataataca tgatgtggtt gaaaatcatg 2221 ttgcagacca actttcatca gacattacac caaatgctat ggatacggaa ttttcagcat 2281 cttctccagc cagtttactg gaacggccaa ccaaTCATAT GGAGGCTCTT GGTCATGACC 2341 ATTTAGGAAC CAATGACCTC ACTGTTGGTG GATTTTTAGA AAATCATGAG GAGCCAAGAG 2401 ATAAAGAACA ATGTGCTGAA GAGAACATAC CAGCATCTTC ACTCAACAAA GGAAAGAAAT 2461 TGATGCATTG CAGAAGCCAT GAAGAGGTCA ATACTGAACT AAAAGCACAA ATAATGAAAG 2521 AGATCCGAAA GCCAGGAAGA AAATATGAAA GAATCTTCAC TTTACTGAAG CATGTGCAAG 2581 GCAGTTTACA AACAAGACTA ATATTTTTAC AAAATGTCAT TAAAGAAGCA TCAAGGTTTA 2641 AAAAACGAAT GCTAATAGAA CAACTGGAGA ACTTCTTGGA TGAAATTCAT CGAAGAGCCA 2701 ATCAGATCAA CCATATTAAT AGCAATTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2761 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2821 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATGGACC 2881 GCACAGCGCC GTTTATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2941 aagatt