Construct: ORF TRCN0000470498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011387.1_s317c1
- Derived from:
- ccsbBroadEn_10736
- DNA Barcode:
- GCCGCGCAAACCTAATCCCCCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLCN2 (1181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470498
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XM_006713490.2 | 75.5% | 75.3% | 1_270del;452C>T;1151_1255del |
| 2 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_001171088.3 | 45.2% | 45.1% | 1_1296del;1478C>T;2177_2281del |
| 3 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XM_006713489.1 | 44.8% | 44.7% | 1_1428del;1610C>T |
| 4 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_001171087.3 | 43.6% | 43.2% | (many diffs) |
| 5 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_004366.6 | 43% | 42.9% | 1_1428del;1610C>T;2309_2413del |
| 6 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_001171089.3 | 42.2% | 41.5% | (many diffs) |
| 7 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XR_001740002.1 | 39% | 1_1608del;1790C>T;2689_2690ins80 | |
| 8 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XM_011512401.1 | 38% | 35.3% | (many diffs) |
| 9 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XR_001740001.1 | 37.5% | (many diffs) | |
| 10 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XM_006521749.3 | 51.5% | 54.6% | (many diffs) |
| 11 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | NM_009900.2 | 36.4% | 38.6% | (many diffs) |
| 12 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XM_017316862.1 | 36.1% | 38.3% | (many diffs) |
| 13 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XR_001781786.1 | 19.8% | (many diffs) | |
| 14 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XR_384520.3 | 12.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcctggttc ccagatggaa ttcatacgga cagcagcacc taccggattg 121 tgcctggggg ctacgctgtg gtcggggcag ctgcgctggc aggagcggtg acacacacag 181 tgtccacggc tgtgatcgtg ttcgagctca caggccagat tgcccacatc ctgcctgtca 241 tgatcgtcgt catcctggcc aacgctgtcg cccagagtct gcagccctcc ctctatgaca 301 gcatcatccg aatcaagaaa ctgccctacc tgcctgagct cggctggggc cgccaccagc 361 agtaccgggt gcgtgtggag gacatcatgg tgcgggatgt tccccatgtg gccctcagct 421 gcaccttccg ggacctgcgt ttggcactgc acaggaccaa gggccgaatg ctggccctag 481 tggagtcccc tgagtccatg attctgctgg gctccatcga gcgttcacag gtggtggcat 541 tgttgggggc ccagctgagc ccagcccgcc ggcggcagca catgcaggag cgcagagcca 601 cccagacctc tccactatct gatcaggagg gtccccctac ccctgaggct tctgtctgct 661 tccaggtgaa cacagaagac tcagccttcc cagcagcccg gggggagacc cacaagcccc 721 taaagcctgc actcaagagg gggcccagtg tcaccaggaa cctcggagag agtcccacag 781 ggagcgcaga gtcggcaggc atcgccctcc ggagccTCTT CTGTGGCAGT CCACCCCCTG 841 AGGCTGCTTC GGAGAAGTTG GAATCCTGTG AGAAGCGCAA GCTGAAGCGT GTCCGAATCT 901 CCCTGGCAAG TGACGCGGAC CTGGAAGGCG AGATGAGCCC TGAAGAGACT CACACTATCT 961 TCTCACTGCT GGGAGTGGAC CATGCTTATG TCACCAGTAT TGGCAGACTC ATTGGAATCG 1021 TTACTCTAAA GGAGCTCCGG AAGGCCATCG AGGGCTCTGT CACAGCACAG GGTGTGAAAG 1081 TCCGGCCGCC CCTCGCCAGC TTCCGAGACA GTGCCACCAG CAGCAGTGAC ACGGAGACCA 1141 CTGAGGTGCA TGCACTCTGG GGGCCCCACT CCCGTCATGG CCTCCCCCGG GAGGGCAGCC 1201 CTTCCGACAG CGACGACAAA TGCCAATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCGCGCA 1381 AACCTAATCC CCCCTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt