Transcript: Human NM_001171089.3

Homo sapiens chloride voltage-gated channel 2 (CLCN2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-07-21
Taxon:
Homo sapiens (human)
Gene:
CLCN2 (1181)
Length:
3157
CDS:
127..2736

Additional Resources:

NCBI RefSeq record:
NM_001171089.3
NBCI Gene record:
CLCN2 (1181)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001171089.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418292 CCTTTGCTGTCATTGGTATTG pLKO_005 1094 CDS 100% 10.800 7.560 N CLCN2 n/a
2 TRCN0000419494 GGGTATCTATGAGAATGAATC pLKO_005 807 CDS 100% 10.800 7.560 N CLCN2 n/a
3 TRCN0000044905 CCTGGTCATCTTCATTCTCAT pLKO.1 1428 CDS 100% 4.950 3.465 N CLCN2 n/a
4 TRCN0000044906 GCAACTAGATGAACCTGTCAA pLKO.1 2457 CDS 100% 4.950 3.465 N CLCN2 n/a
5 TRCN0000044904 CATCACTTTCTCAGCCGGATT pLKO.1 558 CDS 100% 4.050 2.835 N CLCN2 n/a
6 TRCN0000044907 CCCTGAGATGAAGACCATCTT pLKO.1 618 CDS 100% 0.495 0.347 N CLCN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001171089.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10736 pDONR223 100% 42.2% 41.5% None (many diffs) n/a
2 ccsbBroad304_10736 pLX_304 0% 42.2% 41.5% V5 (many diffs) n/a
3 TRCN0000470498 GCCGCGCAAACCTAATCCCCCCTC pLX_317 35.6% 42.2% 41.5% V5 (many diffs) n/a
4 TRCN0000489431 ATATAACCCGGTACCCCGACTGAA pLX_317 35.6% 42.2% 41.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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