Construct: ORF TRCN0000470519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012411.1_s317c1
- Derived from:
- ccsbBroadEn_04820
- DNA Barcode:
- GCCGATTGAGCTCTTAAAAGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3C14 (126961)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 100% | 100% | |
| 2 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 100% | 100% | |
| 3 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 97% | 100% | (many diffs) |
| 4 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 95.8% | 97% | (many diffs) |
| 5 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 95.8% | 97% | (many diffs) |
| 6 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 88.2% | 96.3% | (many diffs) |
| 7 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 86.7% | 99.2% | (many diffs) |
| 8 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 86.7% | 99.2% | (many diffs) |
| 9 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 86.5% | 99.2% | (many diffs) |
| 10 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 85.7% | 99.2% | (many diffs) |
| 11 | human | 8350 | H3C1 | H3 clustered histone 1 | NM_003529.2 | 85.2% | 99.2% | (many diffs) |
| 12 | human | 8353 | H3C6 | H3 clustered histone 6 | NM_003532.2 | 85.2% | 99.2% | (many diffs) |
| 13 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 84.5% | 99.2% | (many diffs) |
| 14 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 84.3% | 99.2% | (many diffs) |
| 15 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 83.4% | 99.2% | (many diffs) |
| 16 | human | 8358 | H3C2 | H3 clustered histone 2 | NM_003537.3 | 82% | 99.2% | (many diffs) |
| 17 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 80.5% | 97% | (many diffs) |
| 18 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 79.7% | 94.1% | (many diffs) |
| 19 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 79% | 97% | (many diffs) |
| 20 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160943.1 | 36.4% | (many diffs) | |
| 21 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 19.9% | (many diffs) | |
| 22 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 17.8% | (many diffs) | |
| 23 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 93.6% | 100% | (many diffs) |
| 24 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 93.6% | 100% | (many diffs) |
| 25 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 93.3% | 100% | (many diffs) |
| 26 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 91.1% | 100% | (many diffs) |
| 27 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 91.1% | 99.2% | (many diffs) |
| 28 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 90.9% | 100% | (many diffs) |
| 29 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 90.9% | 100% | (many diffs) |
| 30 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 90.6% | 100% | (many diffs) |
| 31 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 90.6% | 100% | (many diffs) |
| 32 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 90.6% | 99.2% | (many diffs) |
| 33 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 90.1% | 99.2% | (many diffs) |
| 34 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 90.1% | 99.2% | (many diffs) |
| 35 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 87.5% | 97% | (many diffs) |
| 36 | mouse | 15081 | H3f3b | H3 histone, family 3B | NM_008211.3 | 78.8% | 97% | (many diffs) |
| 37 | mouse | 15081 | H3f3b | H3 histone, family 3B | XM_006532248.2 | 78.8% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC CCGTACTAAG CAGACTGCTC GCAAGTCGAC CGGCGGCAAG GCCCCGAGGA 121 AGCAGCTGGC CACCAAGGCG GCCCGCAAGA GCGCGCCGGC CACGGGCGGG GTGAAGAAGC 181 CGCACCGCTA CCGGCCCGGC ACCGTAGCCC TGCGGGAGAT CCGGCGCTAC CAGAAGTCCA 241 CGGAGCTGCT GATCCGCAAG CTGCCCTTCC AGCGGCTGGT ACGCGAGATC GCGCAGGACT 301 TTAAGACGGA CCTGCGCTTC CAGAGCTCGG CCGTGATGGC GCTGCAGGAG GCCAGCGAGG 361 CCTACCTGGT GGGGCTGTTC GAAGACACGA ACCTGTGCGC CATCCACGCC AAGCGCGTGA 421 CCATTATGCC CAAGGACATC CAGCTGGCCC GCCGCATCCG TGGAGAGCGG GCTTACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAG CCGATTGAGC TCTTAAAAGG AACACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att