Construct: ORF TRCN0000470680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019049.2_s317c1
- Derived from:
- ccsbBroadEn_14622
- DNA Barcode:
- TTTCCTGTACTCAGTCAATTTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.9% | 100% | 2709T>C |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2247del | 99.7% | 99.7% | 2235_2236insGAATTAAGA |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 99.5% | 99.5% | 2237_2238insATTAAGAGAAGCAAC |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 96.2% | 96.1% | 424_425ins135;2574T>C |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 95.5% | 95.6% | 0_1ins159;2550T>C |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 92.5% | 90.3% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.8% | 86.6% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 77.4% | 75.1% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.5% | 50.7% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 51.8% | 51.8% | 1881G>A;1883C>G;1884_1885ins1746 |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.4% | 33.3% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2414 |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.7% | 90.3% | (many diffs) |
| 13 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 44.9% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3699
- ORF length:
- 3630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc 121 tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc 181 tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact 241 gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct 301 tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc 361 gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg 421 ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga 481 gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca 541 acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga 601 tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga 661 gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc 721 agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg 781 caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag 841 ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg 901 atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta 961 attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga 1021 tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta 1081 acggaatagg tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac 1141 acttcaaaaa ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg 1201 gtgactcctt cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg 1261 taaaggaaat cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc 1321 atgcctttga gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc 1381 ttgcagtcgt cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg 1441 atggagatgt gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga 1501 aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca 1561 gctgcaaggc cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc 1621 cggagcccag ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca 1681 agtgcaacct tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt 1741 gccacccaga gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca 1801 actgtatcca gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag 1861 gagtcatggg agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc 1921 acctgtgcca tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa 1981 cgaatgggcc taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc 2041 tggtggtggc cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca 2101 cgctgcggag gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag 2161 ctcccaacca agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc 2221 tgggctccgg tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag 2281 ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg 2341 aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc 2401 tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc ttcggctgcc 2461 tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt 2521 gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc 2581 tggcagccag gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttgggc 2641 tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta 2701 tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct 2761 ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa 2821 tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca 2881 tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc 2941 gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct 3001 accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct 3061 accgtgccct gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca 3121 tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc 3181 tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct 3241 gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga 3301 ctgaggacag catagacgac accttcctcc CAGTGCCTGA ATACATAAAC CAGTCCGTTC 3361 CCAAAAGGCC CGCTGGCTCT GTGCAGAATC CTGTCTATCA CAATCAGCCT CTGAACCCCG 3421 CGCCCAGCAG AGACCCACAC TACCAGGACC CCCACAGCAC TGCAGTGGGC AACCCCGAGT 3481 ATCTCAACAC TGTCCAGCCC ACCTGTGTCA ACAGCACATT CGACAGCCCT GCCCACTGGG 3541 CCCAGAAAGG CAGCCACCAA ATTAGCCTGG ACAACCCTGA CTACCAGCAG GACTTCTTTC 3601 CCAAGGAAGC CAAGCCAAAT GGCATCTTTA AGGGCTCCAC AGCTGAAAAT GCAGAATACC 3661 TAAGGGTCGC GCCACAAAGC AGTGAATTTA TTGGAGCACC AACTTTCTTG TACAAAGTGG 3721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3841 ATTTCCTGTA CTCAGTCAAT TTGTAACGCG TTAAGTCgac aatcaacctc tggattacaa 3901 aatttgtgaa agatt