Construct: ORF TRCN0000470786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016801.1_s317c1
- Derived from:
- ccsbBroadEn_01883
- DNA Barcode:
- AAACACGGTAGAGCCAAGTCGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C9 (8294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 100% | 100% | |
| 2 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 90.2% | 100% | (many diffs) |
| 3 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 90.2% | 100% | (many diffs) |
| 4 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 86% | 100% | (many diffs) |
| 5 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 85.4% | 100% | (many diffs) |
| 6 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 85.4% | 100% | (many diffs) |
| 7 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 85.1% | 100% | (many diffs) |
| 8 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 84.8% | 100% | (many diffs) |
| 9 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 84.4% | 100% | (many diffs) |
| 10 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 84.4% | 100% | (many diffs) |
| 11 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 84.4% | 100% | (many diffs) |
| 12 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 84.1% | 100% | (many diffs) |
| 13 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 83.1% | 100% | (many diffs) |
| 14 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 81.5% | 100% | (many diffs) |
| 15 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 90.2% | 100% | (many diffs) |
| 16 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 90.2% | 100% | (many diffs) |
| 17 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 89.6% | 100% | (many diffs) |
| 18 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 89.6% | 100% | (many diffs) |
| 19 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 88.9% | 100% | (many diffs) |
| 20 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 88.9% | 100% | (many diffs) |
| 21 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 88.9% | 100% | (many diffs) |
| 22 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 88.3% | 100% | (many diffs) |
| 23 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 88.3% | 100% | (many diffs) |
| 24 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 88% | 100% | (many diffs) |
| 25 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 88% | 100% | (many diffs) |
| 26 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 86.4% | 100% | (many diffs) |
| 27 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 85.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 113
- ORF end:
- 422
- ORF length:
- 309
- Sequence:
-
1 TAAGCTCGGG CCCCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA 61 AATTGATGAG CAATGCTTTT TTATAATGCC AACTTTGTAC AAAAAAGTTG GCATGTCAGG 121 ACGCGGCAAA GGAGGTAAGG GCCTGGGGAA AGGGGGTGCC AAGCGCCACC GCAAGGTGCT 181 GCGCGACAAC ATCCAGGGTA TCACCAAGCC AGCCATTCGG CGCCTTGCTC GCCGCGGCGG 241 CGTGAAGCGC ATTTCTGGCC TCATCTATGA GGAGACCCGC GGAGTGTTGA AGGTGTTCCT 301 GGAGAACGTG ATCCGGGACG CCGTGACCTA CACGGAGCAC GCCAAGCGCA AGACGGTCAC 361 CGCCATGGAC GTGGTCTACG CGCTCAAGCG CCAGGGCCGC ACCCTCTATG GCTTCGGCGG 421 CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 481 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 541 GCTTTATATA TCTTGTGGAA AGGACGAAAA CACGGTAGAG CCAAGTCGAC GACGCGTTAA 601 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t