Construct: ORF TRCN0000470812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010907.1_s317c1
- Derived from:
- ccsbBroadEn_12574
- DNA Barcode:
- GGCTGATGGTCTAGTTTCTTTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCNJL (79616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470812
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79616 | CCNJL | cyclin J like | XM_011534646.3 | 100% | 100% | |
| 2 | human | 79616 | CCNJL | cyclin J like | XM_011534647.3 | 87.2% | 83% | (many diffs) |
| 3 | human | 79616 | CCNJL | cyclin J like | XM_017009848.1 | 86.8% | 86.5% | 70_71ins144 |
| 4 | human | 79616 | CCNJL | cyclin J like | XM_006714917.4 | 84.4% | 84.4% | 1_201del |
| 5 | human | 79616 | CCNJL | cyclin J like | NM_024565.7 | 83.9% | 83.9% | 1_210del |
| 6 | human | 79616 | CCNJL | cyclin J like | NM_001308173.2 | 72.8% | 72.6% | 1_210del;280_281ins144 |
| 7 | human | 79616 | CCNJL | cyclin J like | XM_017009847.1 | 68.4% | 40.2% | (many diffs) |
| 8 | human | 79616 | CCNJL | cyclin J like | XM_017009846.2 | 67.9% | 67.9% | 1_516del |
| 9 | human | 79616 | CCNJL | cyclin J like | XR_427810.4 | 30.2% | 1_588del;1266_1472del;1891_3619del | |
| 10 | human | 79616 | CCNJL | cyclin J like | XR_427811.3 | 27% | (many diffs) | |
| 11 | human | 79616 | CCNJL | cyclin J like | NR_131769.2 | 16.7% | (many diffs) | |
| 12 | mouse | 380694 | Ccnjl | cyclin J-like | NM_001045530.2 | 75% | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcgctacaac gtcaccacct ccaagcagct ctacaccgtg gccgtctcct 121 gcctcctgct tgcaaacgga gtttcgctct tatcgcccag gctgaagtgc agtggcatga 181 tctcagctca ttgcaacctc cacctcccag gttcaagcaa ttctcctgct tcagcgcccc 241 accccccacc cacaccgccc caagtagctg agactacagg taagttcgag gatcgggaag 301 accacgtccc caagttggag caaataaaca gcacgaggat cctgagcagc cagaacttca 361 ccctcaccaa gaaggagctg ctgagcacag agctgctgct cctggaggcc ttcagctgga 421 acctctgcct gcccacgcct gcccacttcc tggactacta cctcttggcc tccgtcagcc 481 agaaggacca ccactgccac acctggccca ccacctgccc ccgcaagacc aaagagtgcc 541 tcaaggagta tgcccattac ttcctagagg tcaccctgca agatcacata ttctacaaat 601 tccagccttc tgtggtcgct gcggcctgtg ttggggcctc caggatttgc ctgcagcttt 661 ctccctactg gaccagagac ctgcagagga tctcaagcta ttccctggag cacctcagca 721 cgtgtattga aatcctgctg gtagtgtatg acaacgtcct caaggatgcc GTAGCCGTCA 781 AGAGCCAGGC CTTGGCAATG GTGCCCGGCA CACCCCCCAC CCCCACTCAA GTGCTGTTCC 841 AGCCACCAGC CTACCCGGCC CTCGGCCAGC CAGCGACCAC CCTGGCACAG TTCCAGACCC 901 CCGTGCAGGA CCTATGCTTG GCCTATCGGG ACTCCTTGCA GGCCCACCGT TCAGGGAGCC 961 TGCTCTCGGG GAGTACAGGC TCATCCCTCC ACACCCCGTA CCAACCGCTG CAGCCCTTGG 1021 ATATGTGTCC CGTGCCCGTC CCTGCATCCC TTAGCATGCA TATGGCCATT GCAGCTGAGC 1081 CCAGGCACTG CCTCGCCACC ACCTATGGAA GCAGCTACTT CAGTGGGAGC CACATGTTCC 1141 CCACCGGCTG CTTTGACAGA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGCT GATGGTCTAG 1321 TTTCTTTACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt