Construct: ORF TRCN0000470849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017328.1_s317c1
- Derived from:
- ccsbBroadEn_07805
- DNA Barcode:
- GCCGAACTCATGCTACGTGTAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAF8 (22828)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22828 | SCAF8 | SR-related CTD associated f... | NM_001286199.1 | 99.9% | 99.9% | 890A>T;3048C>T |
| 2 | human | 22828 | SCAF8 | SR-related CTD associated f... | NM_014892.4 | 99.9% | 99.9% | 890A>T;3048C>T |
| 3 | human | 22828 | SCAF8 | SR-related CTD associated f... | NM_001286194.1 | 96.5% | 96.5% | 1_135del;1025A>T;3183C>T |
| 4 | human | 22828 | SCAF8 | SR-related CTD associated f... | NM_001286189.1 | 95% | 94.9% | (many diffs) |
| 5 | human | 22828 | SCAF8 | SR-related CTD associated f... | NM_001286188.1 | 94.1% | 94.1% | 1_234del;1124A>T;3282C>T |
| 6 | mouse | 106583 | Scaf8 | SR-related CTD-associated f... | NM_134123.3 | 88.3% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3879
- ORF length:
- 3813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggccgtgaag accttcaata gcgagttgta ttccctgaat gactataaac 121 cacccatttc gaaagcgaaa atgacccaaa ttactaaggc agccatcaaa gctattaagt 181 tctataaaca tgtggtacag agtgttgaga agtttattca gaaatgtaaa ccagaataca 241 aagtacctgg actttatgtt attgactcca ttgtgcgaca atcccgacat cagtttggtc 301 aagaaaagga tgtgtttgca cccagattta gtaataacat cattagcact ttccagaatt 361 tatatcgttg ccctggggat gacaagagta aaatagtgag agtactaaac ttatggcaga 421 agaataatgt atttaagagt gagattattc agcccctttt ggatatggca gccgggattc 481 cgcctccagt tgtcacacct gttttggcca gcactaccac tgctatgagc aatactccag 541 gaactcctgt gacacctgtt actccggcca atgtggtcca aggcttacct gatccgtggg 601 tatctcagat aacaaataca gatacacttg cggctgtagc tcagatcttg caaagtcctc 661 aaggccagca gcttcaacaa ttaatacaaa ccttacagat acaacaacag aagccccagc 721 cttccattct gcaggcccta gatgctggtc ttgttgttca gttgcaagct cttacggcac 781 aacttacagc tgcagctgca gctgccaaca ctcttactcc cttagaacag ggagtctcct 841 ttaacaagaa gttgatggat aggtttgatt ttggggaaga ctctgagcat agtgaagaac 901 ccaaaaagga aattccagct tcacaacttt ctcacgtttc agaatctgtg aacatttcca 961 tttttcatca gatagcagaa caactacaac agcaaaacct agaacatctc agacagcagc 1021 tcttggagca gcaacagcct caaaaggcca ctcctcagga tagtcaggaa ggaacctttg 1081 ggtcagagca ttcagcgtca ccatcacaag ggagtagtca gcagcatttt cttgaacctg 1141 aagtcaattt ggatgattcc atagatattc agcaacagga tatggatata gatgaagggc 1201 aagatggagt ggaagaggag gtctttgaac aagaagctaa gaaagtggcg gttcgctcaa 1261 gatcaagaac acattcacga tctcgttcaa gatcaccaag aaaacgaagg tctaggtcac 1321 ggtctggctc tagaaagcgt aaacacagaa agcgatcacg ctcccgctca agagaaagaa 1381 agaggaaatc atcacggtcg tattcaagtg aaaggagagc cagagaaagg gagaaagaac 1441 gacagaaaaa gggattacct ccaattagat ctaaaacact aagtgtatgt agtactactc 1501 tctgggttgg gcaagtggac aagaaggcaa cacagcaaga cttaaccaac ctgtttgaag 1561 agtttggaca gattgaatcc attaatatga ttcctccccg gggctgtgct tatgtctgca 1621 tggttcatcg acaagatgca tttcgagctc ttcagaaact cagttctgga tcatataaaa 1681 ttgggtccaa ggtcattaag atcgcttggg ctttaaacaa aggtgtaaaa acagaataca 1741 aacaattctg ggatgtggat cttggagtta catatatacc atgggaaaaa gttaaagtgg 1801 atgacttgga aggttttgca gaaggaggca tgattgatca ggagactgta aatactgagt 1861 gggaaactgt gaaaagctca gaacctgtta aagagacggt ccagacaact cagagcccaa 1921 ctccagttga aaaggagaca gtggtcacaa cccaggcaga ggttttccct cctcctgttg 1981 ctatgttgca gattccagtg gcgccagccg tgcctacagt tagtttagtc ccaccagcat 2041 ttcctgtgtc gatgccggtt cctcctcctg gattcagtcc aatccctcca cctccttttt 2101 taagagcaag ttttaaccct tcacaaccac cacctggttt catgccgcct ccagttcccc 2161 cacctgttgt gccaccccct acgattccac cagtagtacc aacatcttta gtgcagccgt 2221 cattatccat gacaccggaa actgtgaaag atgttggatt tggtagcctt gttataccag 2281 gcggttctgt tgccagcaat cttgctactt ccgctctgcc agctggaaat gtttttaatg 2341 ctccaactaa acaggcagag cctgaagaaa aagtacctca tcttatagac caccagattt 2401 cttctggtga aaacaccaga tcagtgattc caaatgatat ttcaagtaat gctgcaattt 2461 taggaggaca gccgccaaat gtgacaagca attctggaat tctgggagtc caaagaccaa 2521 atgtatcaag taattctgaa attcttgggg tccggccatc taatgtttcc agtagttctg 2581 ggattattgc agcccaacca ccaaatattc taaataactc tggaatattg ggaatacagc 2641 cacccagtgt gtcaaatagt tctggacttt tgggagtgct acccccaaat atacctaaca 2701 attctggact tgtaggagta cagccaccaa atgttccaaa tactcctgga cttctgggaa 2761 cacagccacc agctggacct caaaacttac cccctttaag tatccctaat caaaggatgc 2821 ccacaatgcc aatgttagac attcgtccgg gactaatacc acaggcacct gggccaagat 2881 tccctttaat acagcctgga attccacccc aacggggaat cccaccccca tcggtacttg 2941 attcagctct tcatccacca ccccgtggac cttttcctcc aggagatatt tttagtcaac 3001 cagaaagacc ttttttagct cctggaagac aaagcgtaga caatgttact aacccagaaa 3061 aaaggatacc acttgggaat gataacattc aacaggaagg agatagagat tatcggtttc 3121 ctcctataga aaccagggaa agcattagta gacctccccc tgtggatgtt agagatgtgg 3181 ttgggcggcc tatagatcca agagaaggtc ctggacggcc tccactagat ggtagggatc 3241 attttggaag acctcctgta gatataagag agaatcttgt gaggccaggt atagatcatc 3301 ttggtcgaag agaccacttt ggctttaatc cagagaagcc ctgggggcat agaggagatt 3361 ttgatgagag agagcatcgg gttctaccgg tctatggtgg tccaaaaggc ttacatgaag 3421 aaagaggtag atttcggtct ggaaactatc gatttgatcc tagaagtggt ccttggaacc 3481 gaggatttgg acaagaagtt cacagagatt ttgatgaccg cagaagaccc TGGGAGAGGC 3541 AAAGGGATAG GGATGACAGA GATTTTGATT TCTGCAGAGA AATGAATGGA AATCGTCTTG 3601 GACGAGACAG AATTCAAAAC ACTTGGGTTC CCCCTCCTCA TGCTCGGGTT TTTGATTATT 3661 TTGAAGGGGC CACTTCTCAA CGAAAAGGTG ATAATGTGCC TCAGGTTAAT GGTGAAAATA 3721 CAGAGAGACA TGCTCAGCCA CCACCTATAC CAGTACAGAA TGATCCTGAA CTTTATGAAA 3781 AACTGACATC TTCAAATGAA ATAAACAAGG AGAAGAGTGA CACAGTTGCT GATATAGAAA 3841 GTGAACCAGT GGTAGAAAGC ACAGAAACTG AGGGGACATA CCCAACTTTC TTGTACAAAG 3901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 4021 ACGAGCCGAA CTCATGCTAC GTGTAGACAC GCGTTAAGTC gacaatcaac ctctggatta 4081 caaaatttgt gaaagatt