Construct: ORF TRCN0000470878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016882.1_s317c1
- Derived from:
- ccsbBroadEn_13737
- DNA Barcode:
- ATACAACTAGATCCCAGAAAGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHAP2 (727956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220729 | LOC220729 | succinate dehydrogenase com... | NR_003266.2 | 27.3% | (many diffs) | |
| 2 | human | 6389 | SDHA | succinate dehydrogenase com... | NM_001330758.1 | 23.7% | 22.9% | (many diffs) |
| 3 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_011514073.2 | 23% | 22.3% | (many diffs) |
| 4 | human | 6389 | SDHA | succinate dehydrogenase com... | NM_004168.4 | 20.8% | 20.1% | (many diffs) |
| 5 | human | 6389 | SDHA | succinate dehydrogenase com... | NM_001294332.1 | 20.6% | 18.1% | (many diffs) |
| 6 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_011514072.2 | 20.3% | 19.7% | (many diffs) |
| 7 | human | 727956 | SDHAP2 | succinate dehydrogenase com... | NR_003265.3 | 19% | (many diffs) | |
| 8 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_017009685.2 | 17.6% | 17% | (many diffs) |
| 9 | human | 6389 | SDHA | succinate dehydrogenase com... | XM_024446143.1 | 17.3% | 15.1% | (many diffs) |
| 10 | human | 255812 | SDHAP1 | succinate dehydrogenase com... | NR_003264.2 | 16.8% | (many diffs) | |
| 11 | human | 6389 | SDHA | succinate dehydrogenase com... | XR_002956167.1 | 7.9% | (many diffs) | |
| 12 | mouse | 66945 | Sdha | succinate dehydrogenase com... | NM_023281.1 | 19.4% | 19.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 513
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggaggac aactggaggt ggcatttcta tgacaccgtg aagggctccg 121 actggctggg ggaccaggat gccatccact acgtgacgga gcaggccccc actgCCATGG 181 TCGAGGTAGA AAATTATGGC ATGCCGTTTA GCAGAACTGA AGATGGGAAG ATTTATCAGC 241 GTGCATTTGG CGGACACAGC CTCAAGTTTG GAAAGGGCAG GCAGGCCCAT CGGTGCTGCT 301 GTGTGGCTGA TCGGACCGGC CACTCAATAT TGCACACCTT ATATGGGAGG TCTCTGCGAT 361 ATGATACCAG CTGTTTTGTG GAGTATTTTG CCTTGGATCT CCTGATGGAG AATGGGGAGT 421 GCCGTGGTGT CTTCGCACTG TGCATACAGG ACGGGTCCAT CCATCGCATA AGAGCAAAGA 481 ATACTATTGT TGCCACAGGG TACTTGGGAG GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 661 ACAACTAGAT CCCAGAAAGA CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt