Construct: ORF TRCN0000471064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009490.1_s317c1
- Derived from:
- ccsbBroadEn_04718
- DNA Barcode:
- TAACGGACTGCAAGGTTGAAGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL39L (116832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471064
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116832 | RPL39L | ribosomal protein L39 like | NM_052969.3 | 100% | 100% | |
| 2 | human | 6170 | RPL39 | ribosomal protein L39 | NM_001000.4 | 94.7% | 92.1% | (many diffs) |
| 3 | human | 170958 | ZNF525 | zinc finger protein 525 | NR_145447.2 | 7.3% | (many diffs) | |
| 4 | human | 170958 | ZNF525 | zinc finger protein 525 | NR_145446.2 | 6.4% | (many diffs) | |
| 5 | human | 388558 | ZNF808 | zinc finger protein 808 | XR_935832.3 | 3.1% | (many diffs) | |
| 6 | human | 388558 | ZNF808 | zinc finger protein 808 | XR_002958314.1 | 3% | (many diffs) | |
| 7 | human | 170958 | ZNF525 | zinc finger protein 525 | NR_145445.2 | 2.6% | (many diffs) | |
| 8 | mouse | 68172 | Rpl39l | ribosomal protein L39-like | NM_026594.2 | 88.2% | 90.1% | (many diffs) |
| 9 | mouse | 68172 | Rpl39l | ribosomal protein L39-like | XM_006522506.3 | 88.2% | 90.1% | (many diffs) |
| 10 | mouse | 67248 | Rpl39 | ribosomal protein L39 | NM_026055.2 | 86.2% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 467
- ORF length:
- 153
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGTCTT CTCACAAGAC TTTCACCATT AAGCGATTCC TGGCCAAGAA 361 ACAAAAGCAA AATCGTCCCA TCCCCCAGTG GATTCAGATG AAACCTGGTA GTAAAATCAG 421 GTACAACTCC AAAAGGAGGC ATTGGAGAAG AACCAAGCTG GGTCTATACC CAACTTTCTT 481 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 541 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 601 TGGAAAGGAC GATAACGGAC TGCAAGGTTG AAGAACACGC GTTAAGTCga caatcaacct 661 ctggattaca aaatttgtga aagatt