Transcript: Human NR_145445.2

Homo sapiens zinc finger protein 525 (ZNF525), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
ZNF525 (170958)
Length:
5220
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_145445.2
NBCI Gene record:
ZNF525 (170958)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_145445.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000021905 GACGTGATGCTGGAGAATTAT pLKO.1 225 3UTR 100% 15.000 7.500 Y ZNF765 n/a
2 TRCN0000018107 GCTGGAAACAAGCCTATTAAA pLKO.1 513 3UTR 100% 15.000 7.500 Y ZNF600 n/a
3 TRCN0000021906 CCCTGCTCAGAGGACTCTATA pLKO.1 200 3UTR 100% 13.200 6.600 Y ZNF765 n/a
4 TRCN0000148017 GAGAAACCTTACAGGTGTAAT pLKO.1 1764 3UTR 100% 13.200 6.600 Y ZNF813 n/a
5 TRCN0000424956 AGCGATTCCTGGCCAAGAAAC pLKO_005 4891 3UTR 100% 10.800 5.400 Y Rpl39 n/a
6 TRCN0000117635 CGATTCCTGGCCAAGAAACAA pLKO.1 4893 3UTR 100% 5.625 2.813 Y RPL39 n/a
7 TRCN0000333551 CGATTCCTGGCCAAGAAACAA pLKO_005 4893 3UTR 100% 5.625 2.813 Y RPL39 n/a
8 TRCN0000149130 GCTGGAGAGAAACCTTACAAA pLKO.1 2342 3UTR 100% 5.625 2.813 Y ZNF761 n/a
9 TRCN0000015887 GTGAAGAATGTGACAAAGTTT pLKO.1 2947 3UTR 100% 5.625 2.813 Y ZNF702P n/a
10 TRCN0000021908 TCAGGGATGTGGCCATAGAAT pLKO.1 151 3UTR 100% 5.625 2.813 Y ZNF765 n/a
11 TRCN0000146631 CCTCATTAGACATCAGAGAAT pLKO.1 3484 3UTR 100% 4.950 2.475 Y ZNF816 n/a
12 TRCN0000150044 CCTTGAAAGACATAGGAGAAT pLKO.1 1733 3UTR 100% 4.950 2.475 Y ZNF816 n/a
13 TRCN0000117636 GAAGAACCAAGCTGGGTCTAT pLKO.1 4996 3UTR 100% 4.950 2.475 Y RPL39 n/a
14 TRCN0000333490 GAAGAACCAAGCTGGGTCTAT pLKO_005 4996 3UTR 100% 4.950 2.475 Y RPL39 n/a
15 TRCN0000015885 GCAATTCATACTGGAGAGAAA pLKO.1 2501 3UTR 100% 4.950 2.475 Y ZNF702P n/a
16 TRCN0000150214 GTAATGAATGTGGCAAGGTTT pLKO.1 2532 3UTR 100% 4.950 2.475 Y ZNF813 n/a
17 TRCN0000117634 AGAAGAACCAAGCTGGGTCTA pLKO.1 4995 3UTR 100% 4.050 2.025 Y RPL39 n/a
18 TRCN0000021904 GACTCTATACAGGGACGTGAT pLKO.1 212 3UTR 100% 4.050 2.025 Y ZNF765 n/a
19 TRCN0000117633 GAGACATTGGAGAAGAACCAA pLKO.1 4985 3UTR 100% 3.000 1.500 Y RPL39 n/a
20 TRCN0000151709 CAAATGTAATGAGTGTGGCAA pLKO.1 1439 3UTR 100% 2.640 1.320 Y ZNF320 n/a
21 TRCN0000021907 GCTGGAGAATTATAGGAACCT pLKO.1 233 3UTR 100% 2.640 1.320 Y ZNF765 n/a
22 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1008 3UTR 100% 4.950 2.475 Y ZNF28 n/a
23 TRCN0000147979 GCAATTCATACTGGAGAGAAT pLKO.1 2501 3UTR 100% 4.950 2.475 Y ZNF321P n/a
24 TRCN0000117652 GCACACATATTTATGCTGTAT pLKO.1 5037 3UTR 100% 4.950 2.475 Y RPL39L n/a
25 TRCN0000333154 GCACACATATTTATGCTGTAT pLKO_005 5037 3UTR 100% 4.950 2.475 Y RPL39L n/a
26 TRCN0000148611 CGTAGACTTCATACTGGAGAA pLKO.1 1326 3UTR 100% 4.050 2.025 Y ZNF761 n/a
27 TRCN0000149655 GCCCTTGTAATTCATAAGGCT pLKO.1 2483 3UTR 100% 0.750 0.375 Y ZNF813 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_145445.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08581 pDONR223 100% 23.1% None (many diffs) n/a
2 ccsbBroad304_08581 pLX_304 0% 23.1% V5 (many diffs) n/a
3 TRCN0000476486 AGTATTTAAATCGATGTCTATAAC pLX_317 30.4% 23.1% V5 (many diffs) n/a
4 ccsbBroadEn_10493 pDONR223 100% 10.4% None (many diffs) n/a
5 ccsbBroad304_10493 pLX_304 0% 10.4% V5 (many diffs) n/a
6 TRCN0000473858 GACTCTAACGAATGTATACCTGAC pLX_317 78.5% 10.4% V5 (many diffs) n/a
7 ccsbBroadEn_05629 pDONR223 100% 8.4% None (many diffs) n/a
8 ccsbBroad304_05629 pLX_304 0% 8.4% V5 (many diffs) n/a
9 TRCN0000468214 TCTCTAGTACCTCAATAGGTGGTT pLX_317 94.7% 8.4% V5 (many diffs) n/a
10 ccsbBroadEn_12729 pDONR223 100% 7.4% None (many diffs) n/a
11 ccsbBroad304_12729 pLX_304 0% 7.4% V5 (many diffs) n/a
12 TRCN0000465897 CCCGCAATCACAACGATCTCAAAA pLX_317 70.4% 7.4% V5 (many diffs) n/a
13 ccsbBroadEn_14339 pDONR223 100% 3.2% None (many diffs) n/a
14 ccsbBroad304_14339 pLX_304 0% 3.2% V5 (not translated due to prior stop codon) (many diffs) n/a
15 TRCN0000478280 ACGAGTTCACTGTCATGACCAACC pLX_317 100% 3.2% V5 (not translated due to prior stop codon) (many diffs) n/a
16 ccsbBroadEn_01440 pDONR223 95.9% 2.7% None (many diffs) n/a
17 ccsbBroad304_01440 pLX_304 0% 2.7% V5 (not translated due to prior stop codon) (many diffs) n/a
18 ccsbBroadEn_04718 pDONR223 100% 2.6% None (many diffs) n/a
19 ccsbBroad304_04718 pLX_304 0% 2.6% V5 (many diffs) n/a
20 TRCN0000471064 TAACGGACTGCAAGGTTGAAGAAC pLX_317 100% 2.6% V5 (many diffs) n/a
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