Transcript: Human NM_001352745.2

Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 56, mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Length:
4670
CDS:
363..3104

Additional Resources:

NCBI RefSeq record:
NM_001352745.2
NBCI Gene record:
PTK2 (5747)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146703 ATGTGGGAGATACTGATGCA pXPR_003 TGG 1424 52% 20 1.1212 PTK2 PTK2 75544
2 BRDN0001148426 AGAGCAAAAGATTTGTACAC pXPR_003 AGG 1203 44% 17 0.9553 PTK2 PTK2 75545
3 BRDN0001145734 TCTGATGATAAATGACTGCG pXPR_003 AGG 619 23% 9 -0.0518 PTK2 PTK2 75543
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001352745.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121318 CCGATTGGAAACCAACATATA pLKO.1 2496 CDS 100% 13.200 18.480 N PTK2 n/a
2 TRCN0000332999 CCGATTGGAAACCAACATATA pLKO_005 2496 CDS 100% 13.200 18.480 N PTK2 n/a
3 TRCN0000196310 GATGTTGGTTTAAAGCGATTT pLKO.1 621 CDS 100% 10.800 15.120 N PTK2 n/a
4 TRCN0000344535 GATGTTGGTTTAAAGCGATTT pLKO_005 621 CDS 100% 10.800 15.120 N PTK2 n/a
5 TRCN0000196667 GCGATTATATGTTAGAGATAG pLKO.1 481 CDS 100% 10.800 15.120 N PTK2 n/a
6 TRCN0000001620 CCGGTCGAATGATAAGGTGTA pLKO.1 2699 CDS 100% 4.050 5.670 N PTK2 n/a
7 TRCN0000121211 CTTTGGATTATCCCGATATAT pLKO.1 1628 CDS 100% 15.000 12.000 N PTK2 n/a
8 TRCN0000194984 CAACAGGTGAAGAGCGATTAT pLKO.1 468 CDS 100% 13.200 10.560 N PTK2 n/a
9 TRCN0000344599 CAACAGGTGAAGAGCGATTAT pLKO_005 468 CDS 100% 13.200 10.560 N PTK2 n/a
10 TRCN0000194944 CACACTTTGATTTGGGTTCAT pLKO.1 3266 3UTR 100% 4.950 3.960 N PTK2 n/a
11 TRCN0000121320 GTCTAGAAATACGGCGATCAT pLKO.1 541 CDS 100% 4.950 3.960 N PTK2 n/a
12 TRCN0000121321 CTTGGCCCTGAGGACATTATT pLKO.1 2819 CDS 100% 15.000 10.500 N PTK2 n/a
13 TRCN0000195014 CAATATGCTAATCCCACTTTA pLKO.1 3601 3UTR 100% 13.200 9.240 N PTK2 n/a
14 TRCN0000121131 CCTAACCATTGCGGAGAATAT pLKO.1 914 CDS 100% 13.200 9.240 N PTK2 n/a
15 TRCN0000121129 GCCCAGGTTTACTGAACTTAA pLKO.1 1934 CDS 100% 13.200 9.240 N PTK2 n/a
16 TRCN0000344601 GCCCAGGTTTACTGAACTTAA pLKO_005 1934 CDS 100% 13.200 9.240 N PTK2 n/a
17 TRCN0000196611 GAGATGTACATCAAGGCATTT pLKO.1 1237 CDS 100% 10.800 7.560 N PTK2 n/a
18 TRCN0000023486 CCAACCTTAATAGAGAAGAAA pLKO.1 718 CDS 100% 5.625 3.938 N Ptk2 n/a
19 TRCN0000121128 CCAGGGATTATGAGATTCAAA pLKO.1 1171 CDS 100% 5.625 3.938 N PTK2 n/a
20 TRCN0000023487 CGAGTATTAAAGGTCTTTCAT pLKO.1 293 5UTR 100% 5.625 3.938 N Ptk2 n/a
21 TRCN0000194838 CCTAAGAGTTTACTGGATTCT pLKO.1 645 CDS 100% 4.950 3.465 N PTK2 n/a
22 TRCN0000121209 CCTCATATTGTGAAGCTGATT pLKO.1 1377 CDS 100% 4.950 3.465 N PTK2 n/a
23 TRCN0000001618 CGGAGAATATGGCTGACCTAA pLKO.1 925 CDS 100% 4.950 3.465 N PTK2 n/a
24 TRCN0000121127 CGTGTGGATATGTGAAGCATT pLKO.1 3707 3UTR 100% 4.950 3.465 N PTK2 n/a
25 TRCN0000121319 GCCCAGAAGAAGGAATCAGTT pLKO.1 847 CDS 100% 4.950 3.465 N PTK2 n/a
26 TRCN0000023485 GCCTTAACAATGCGTCAGTTT pLKO.1 1350 CDS 100% 4.950 3.465 N Ptk2 n/a
27 TRCN0000121130 GCCTTAACAATGCGTCAGTTT pLKO.1 1350 CDS 100% 4.950 3.465 N PTK2 n/a
28 TRCN0000350696 GCCTTAACAATGCGTCAGTTT pLKO_005 1350 CDS 100% 4.950 3.465 N Ptk2 n/a
29 TRCN0000195611 CGATGTCATTGACCAAGCAAG pLKO.1 3050 CDS 100% 4.050 2.835 N PTK2 n/a
30 TRCN0000001621 CGACAGCAACAGGAAATGGAA pLKO.1 2376 CDS 100% 3.000 2.100 N PTK2 n/a
31 TRCN0000121317 GCAATGTTATTTCTCTTGGAA pLKO.1 3803 3UTR 100% 3.000 2.100 N PTK2 n/a
32 TRCN0000001617 GAGAGCATGAAGCAAAGAATT pLKO.1 3489 3UTR 100% 0.000 0.000 N PTK2 n/a
33 TRCN0000121207 CCCAGGAGAGAATGAAGCAAA pLKO.1 3984 3UTR 100% 4.950 2.970 N PTK2 n/a
34 TRCN0000344602 CCCAGGAGAGAATGAAGCAAA pLKO_005 3984 3UTR 100% 4.950 2.970 N PTK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001352745.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14818 pDONR223 0% 85% 84.3% None (many diffs) n/a
2 ccsbBroad304_14818 pLX_304 0% 85% 84.3% V5 (many diffs) n/a
3 TRCN0000471115 ACCCGTCAGACGGGGAGAATATCA pLX_317 13% 85% 84.3% V5 (many diffs) n/a
4 TRCN0000489116 AGGTTGAACTATACACTATACCTG pLX_317 12.9% 85% 84.3% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_13934 pDONR223 100% 73.1% 72% None (many diffs) n/a
6 ccsbBroad304_13934 pLX_304 0% 73.1% 72% V5 (many diffs) n/a
7 TRCN0000465393 CCGAGCTAACCTATAGCACCACCA pLX_317 11.8% 73.1% 72% V5 (many diffs) n/a
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