Construct: ORF TRCN0000471221
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008606.1_s317c1
- Derived from:
- ccsbBroadEn_11315
- DNA Barcode:
- CTCTTGCTCCGCTGCCTTCCCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST3GAL5 (8869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471221
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354227.2 | 99.9% | 99.7% | 227A>G |
| 2 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354229.2 | 99.9% | 99.7% | 227A>G |
| 3 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354238.1 | 99.9% | 99.7% | 227A>G |
| 4 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001042437.2 | 98.6% | 98.4% | 1_15del;242A>G |
| 5 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_003896.4 | 93.2% | 93% | 1_84del;311A>G |
| 6 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005202.2 | 86.3% | 86.1% | 227A>G;764_765ins159 |
| 7 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001363847.1 | 80.5% | 80.3% | 1_84del;311A>G;848_849ins159 |
| 8 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354223.2 | 74.3% | 74.3% | 0_1ins300 |
| 9 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354224.2 | 74.3% | 74.3% | 0_1ins300 |
| 10 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354226.2 | 74.3% | 74.3% | 0_1ins300 |
| 11 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354233.2 | 74.3% | 74.3% | 0_1ins300 |
| 12 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354234.1 | 74.3% | 74.3% | 0_1ins300 |
| 13 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354248.1 | 74.3% | 74.3% | 0_1ins300 |
| 14 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005203.2 | 74.3% | 74.3% | 0_1ins300 |
| 15 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005204.2 | 74.3% | 74.3% | 0_1ins300 |
| 16 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005205.2 | 74.3% | 74.3% | 0_1ins300 |
| 17 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005206.2 | 74.3% | 74.3% | 0_1ins300 |
| 18 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005208.2 | 74.3% | 74.3% | 0_1ins300 |
| 19 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005209.1 | 74.3% | 74.3% | 0_1ins300 |
| 20 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005212.2 | 74.3% | 74.3% | 0_1ins300 |
| 21 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005213.2 | 74.3% | 74.3% | 0_1ins300 |
| 22 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XM_017005214.2 | 60.7% | 60.7% | 0_1ins300;464_465ins159 |
| 23 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XR_001739019.1 | 46.4% | (many diffs) | |
| 24 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | NM_001354247.1 | 45.3% | 45.3% | 0_1ins639 |
| 25 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XR_001739020.1 | 38.8% | (many diffs) | |
| 26 | human | 8869 | ST3GAL5 | ST3 beta-galactoside alpha-... | XR_001739021.1 | 34.7% | (many diffs) | |
| 27 | mouse | 20454 | St3gal5 | ST3 beta-galactoside alpha-... | NM_011375.3 | 84.1% | 85.1% | (many diffs) |
| 28 | mouse | 20454 | St3gal5 | ST3 beta-galactoside alpha-... | XM_006505817.3 | 84.1% | 85.1% | (many diffs) |
| 29 | mouse | 20454 | St3gal5 | ST3 beta-galactoside alpha-... | NM_001035228.2 | 78.6% | 79.6% | (many diffs) |
| 30 | mouse | 20454 | St3gal5 | ST3 beta-galactoside alpha-... | XM_006505816.3 | 64.5% | 65.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aagtgagtac acctatgtga aactgagaag tgattgctcg aggccttccc 121 tgcaatggta cacccgagct caaagcaaga tgagaaggcc cagcttgtta ttaaaagaca 181 tcctcaaatg tacattgctt gtgtttggag tgtggatcct ttatatcctc aagttaaatt 241 atactactga agaatgtgac atgaaaaaaa tgcattatgt ggaccctgac cgtgtaaaga 301 gagctcagaa atatgctcag caagtcttgc agaaggaatg tcgtcccaag tttgccaaga 361 catcaatggc gctgttattt gagcacaggt atagcgtgga cttactccct tttgtgcaga 421 aggcccccaa agacagtgaa gctgagtcca agtacgatcc tccttttggg ttccggaagt 481 tctccagtaa agtccagacc ctcttggaac tcttgccaga gcacgacctc cctgaacact 541 tgaaagccaa gacctgtcgg cgctgtgtgg ttattggaag cggaggaata ctgcacggat 601 tagaactggg ccacaccctg aaccagttcg atgttgtgat aaggttaaac agtgcaccag 661 ttgagggata ttcagaacat gttggaaata aaactactat aaggatgact tatccagagg 721 gcgcaccact gtctgacctt gaatattatt ccaatgactt atttgttgct gttttattta 781 agagtgttGA TTTCAACTGG CTTCAAGCAA TGGTAAAAAA GGAAACCCTG CCATTCTGGG 841 TACGACTCTT CTTTTGGAAG CAGGTGGCAG AAAAAATCCC ACTGCAGCCA AAACATTTCA 901 GGATTTTGAA TCCAGTTATC ATCAAAGAGA CTGCCTTTGA CATCCTTCAG TACTCAGAGC 961 CTCAGTCAAG GTTCTGGGGC CGAGATAAGA ACGTCCCCAC AATCGGTGTC ATTGCCGTTG 1021 TCTTAGCCAC ACATCTGTGC GATGAAGTCA GTTTGGCGGG TTTTGGATAT GACCTCAATC 1081 AACCCAGAAC ACCTTTGCAC TACTTCGACA GTCAATGCAT GGCTGCTATG AACTTTCAGA 1141 CCATGCATAA TGTGACAACG GAAACCAAGT TCCTCTTAAA GCTGGTCAAA GAGGGAGTGG 1201 TGAAAGATCT CAGTGGAGGC ATTGATCGTG AATTTTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 ACTCTTGCTC CGCTGCCTTC CCTAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt