Construct: ORF TRCN0000471228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008133.1_s317c1
- Derived from:
- ccsbBroadEn_01576
- DNA Barcode:
- ATTGCGAATAGACCTACGTGCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOAT1 (6646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NM_003101.6 | 100% | 100% | |
| 2 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | XM_011509911.1 | 100% | 100% | |
| 3 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NM_001252511.1 | 89.3% | 89.2% | 0_1ins175;2delT |
| 4 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NM_001252512.1 | 88.1% | 88.1% | 0_1ins195 |
| 5 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | XM_011509912.1 | 60.1% | 60.1% | 990_990delGinsCTTTGGT;993_994ins651 |
| 6 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NR_045530.2 | 22.6% | (many diffs) | |
| 7 | mouse | 20652 | Soat1 | sterol O-acyltransferase 1 | NM_009230.3 | 82.2% | 85.4% | (many diffs) |
| 8 | mouse | 20652 | Soat1 | sterol O-acyltransferase 1 | XM_006496719.3 | 77.7% | 81.4% | (many diffs) |
| 9 | mouse | 20652 | Soat1 | sterol O-acyltransferase 1 | XM_011238778.2 | 77.7% | 81.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gggtgaagag aagatgtctc taagaaaccg gctgtcaaag tccagggaaa 121 atcctgagga agatgaagac cagagaaacc ctgcaaagga gtccctagag acacctagta 181 atggtcgaat tgacataaaa cagttgatag caaagaagat aaagttgaca gcagaggcag 241 aggaattgaa gccatttttt atgaaggaag ttggcagtca ctttgatgat tttgtgacca 301 atctcattga aaagtcagca tcattagata atggtgggtg cgctctcaca accttttctg 361 ttcttgaagg agagaaaaac aaccatagag cgaaggattt gagagcacct ccagaacaag 421 gaaagatttt tattgcaagg cgctctctct tagatgaact gcttgaagtg gaccacatca 481 gaacaatata tcacatgttt attgccctcc tcattctctt tatcctcagc acacttgtag 541 tagattacat tgatgaagga aggctggtgc ttgagttcag cctcctgtct tatgcttttg 601 gcaaatttcc taccgttgtt tggacctggt ggatcatgtt cctgtctaca ttttcagttc 661 cctattttct gtttcaacat tgggccactg gctatagcaa gagttctcat ccgctgatcc 721 gttctctctt ccatggcttt cttttcatga tcttccagat tggagttcta ggttttggac 781 caacatatgt tgtgttagca tatacactgc caccagcttc ccggttcatc attatattcg 841 agcagattcg ttttgtaatg aaggcccact catttgtcag agagaacgtg cctcgggtac 901 taaattcagc taaggagaaa tcaagcactg ttccaatacc tacagtcaac cagtatttgt 961 acttcttatt tgctcctacc cttatctacc gtgacagcta tcccaggaat cccactgtaa 1021 gatggggtta tgtcgctatg aagtttgcac aggtctttgg ttgctttttc tatgtgtact 1081 acatctttga aaggctttgt gcccccttgt ttcggaatat caaacaggag cccttcagcg 1141 ctcgtgttct ggtcctatgt gtatttaact ccatcttgcc aggtgtgctg attctcttcc 1201 ttactttttt tgcctttttg cactgctggc tcaatgcctt tgctgagatg ttacgctttG 1261 GTGACAGGAT GTTCTATAAG GATTGGTGGA ACTCCACGTC ATACTCCAAC TATTATAGAA 1321 CCTGGAATGT GGTGGTCCAT GACTGGCTAT ATTACTATGC TTACAAGGAC TTTCTCTGGT 1381 TTTTCTCCAA GAGATTCAAA TCTGCTGCCA TGTTAGCTGT CTTTGCTGTA TCTGCTGTAG 1441 TACACGAATA TGCCTTGGCT GTTTGCTTGA GCTTTTTCTA TCCCGTGCTC TTCGTGCTCT 1501 TCATGTTCTT TGGAATGGCT TTCAACTTCA TTGTCAATGA TAGTCGGAAA AAGCCGATTT 1561 GGAATGTTCT GATGTGGACT TCTCTTTTCT TGGGCAATGG AGTCTTACTC TGCTTTTATT 1621 CTCAAGAATG GTATGCACGT CAGCACTGTC CTCTGAAAAA TCCCACATTT TTGGATTATG 1681 TCCGGCCACG TTCCTGGACT TGTCGTTACG TGTTTTACCC AACTTTCTTG TACAAAGTGG 1741 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1801 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1861 AATTGCGAAT AGACCTACGT GCCATACGCG TTAAGTCgac aatcaacctc tggattacaa 1921 aatttgtgaa agatt