Transcript: Mouse XM_011238778.2

PREDICTED: Mus musculus sterol O-acyltransferase 1 (Soat1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Soat1 (20652)
Length:
4752
CDS:
315..1838

Additional Resources:

NCBI RefSeq record:
XM_011238778.2
NBCI Gene record:
Soat1 (20652)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011238778.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197536 CCAACCAGAGACTAAACATAT pLKO.1 2137 3UTR 100% 13.200 9.240 N Soat1 n/a
2 TRCN0000336269 CCAACCAGAGACTAAACATAT pLKO_005 2137 3UTR 100% 13.200 9.240 N Soat1 n/a
3 TRCN0000336399 GAATATCCCACGAGTACTAAA pLKO_005 1004 CDS 100% 13.200 9.240 N Soat1 n/a
4 TRCN0000336332 GTTCTCACCCATTGATCTATT pLKO_005 823 CDS 100% 13.200 9.240 N Soat1 n/a
5 TRCN0000178477 GCGTAGTGACTTTCCTTGTTA pLKO.1 2932 3UTR 100% 5.625 3.938 N Soat1 n/a
6 TRCN0000353388 GCGTAGTGACTTTCCTTGTTA pLKO_005 2932 3UTR 100% 5.625 3.938 N Soat1 n/a
7 TRCN0000177641 CCAATCTGGAACATCATGGTT pLKO.1 1674 CDS 100% 3.000 2.100 N Soat1 n/a
8 TRCN0000197396 CCTTTGGTCTAATTGCTTTAT pLKO.1 2903 3UTR 100% 13.200 7.920 N Soat1 n/a
9 TRCN0000336330 CCTTTGGTCTAATTGCTTTAT pLKO_005 2903 3UTR 100% 13.200 7.920 N Soat1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011238778.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01576 pDONR223 100% 77.7% 81.4% None (many diffs) n/a
2 ccsbBroad304_01576 pLX_304 0% 77.7% 81.4% V5 (many diffs) n/a
3 TRCN0000471228 ATTGCGAATAGACCTACGTGCCAT pLX_317 27.9% 77.7% 81.4% V5 (many diffs) n/a
4 TRCN0000488886 TAAGCCTAATATATTGCACCGCGA pLX_317 24.2% 77.7% 81.4% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000488688 CACCGAGCGGTTAATCGTTAAAGT pLX_317 22.8% 77.6% 81.3% V5 (many diffs) n/a
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