Construct: ORF TRCN0000471351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012982.1_s317c1
- Derived from:
- ccsbBroadEn_07554
- DNA Barcode:
- ATAATAAATACATTTACAGATCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTR1B (10120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471351
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10120 | ACTR1B | actin related protein 1B | NM_005735.4 | 99.8% | 99.7% | 278T>C;804C>G |
| 2 | human | 10120 | ACTR1B | actin related protein 1B | XM_017003116.1 | 88.1% | 88% | 0_1ins132;146T>C;672C>G |
| 3 | human | 10120 | ACTR1B | actin related protein 1B | XM_005263854.5 | 80.1% | 80% | 0_1ins222;56T>C;582C>G |
| 4 | human | 10121 | ACTR1A | actin related protein 1A | NM_005736.4 | 79.5% | 90.1% | (many diffs) |
| 5 | mouse | 226977 | Actr1b | ARP1 actin-related protein ... | NM_146107.2 | 89.2% | 98.6% | (many diffs) |
| 6 | mouse | 226977 | Actr1b | ARP1 actin-related protein ... | XM_011238484.1 | 80.4% | 86.6% | (many diffs) |
| 7 | mouse | 54130 | Actr1a | ARP1 actin-related protein ... | NM_016860.1 | 79.6% | 90.1% | (many diffs) |
| 8 | mouse | 226977 | Actr1b | ARP1 actin-related protein ... | XM_011238485.2 | 78.4% | 86.9% | (many diffs) |
| 9 | mouse | 226977 | Actr1b | ARP1 actin-related protein ... | XM_011238486.2 | 70.9% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtcctacgac atcatcgcca accagcctgt ggtcatcgac aacggttcgg 121 gggtgattaa agctggcttt gcaggagacc agattcccaa atactgtttc ccaaactatg 181 tcgggcggcc gaagcacatg cgggtgatgg ctggagccct ggagggggac ctcttcatcg 241 gaccaaaagc agaggagcac cgggggctgc tgaccatccg ctaccccatg gagcacggcg 301 tggtgcgaga ctggaacgac atggaacgca tctggcagta cgcctactcc aaggatcagc 361 tgcagacctt ctcggaggag catcctgtgc tcctcacgga ggccccgctc aacccgagta 421 agaaccggga gaaggcggca gaggtgttct ttgagacctt caacgtgccg gccctgttca 481 tctccatgca ggctgtgctc agtctgtacg caacaggacg cacgacagga gtggttctag 541 actcagggga cggggtcact catgctgtgc ccatctatga gggctttgcc atgcctcact 601 ccatcatgcg ggtggacatt gccggccgcg acgtctcccg ctacctccga ctcctgctgc 661 gcaaggaagg ggttgacttc cataccTCGG CTGAGTTTGA GGTTGTCCGG ACAATCAAAG 721 AGCGAGCGTG CTACCTGTCC ATCAACCCAC AGAAGGATGA GGCTCTGGAG ACGGAGAAGG 781 TGCAGTACAC GTTGCCAGAC GGCAGCACGC TTGATGTGGG GCCTGCACGA TTCCGGGCCC 841 CCGAGCTGCT GTTCCAGCCG GACCTTGTGG GGGATGAGAG TGAGGGGCTC CATGAGGTGG 901 TGGCCTTCGC CATACACAAG TCCGACATGG ACCTGCGCCG GACGCTGTTC GCCAACATCG 961 TGCTCTCAGG TGGCTCAACG CTTTTCAAAG GCTTCGGAGA CCGATTACTC AGTGAAGTGA 1021 AGAAGCTTGC CCCAAAGGAT ATCAAAATCA AGATCTCAGC CCCGCAGGAA CGGCTGTACT 1081 CCACATGGAT TGGCGGCTCC ATCCTGGCCT CGCTGGACAC TTTTAAGAAG ATGTGGGTGT 1141 CCAAAAAGGA GTATGAAGAG GATGGCTCCC GTGCTATTCA TCGCAAAACT TTCTACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAA TAATAAATAC ATTTACAGAT CGAACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att