Construct: ORF TRCN0000471379

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008264.1_s317c1
Derived from:
ccsbBroadEn_01278
DNA Barcode:
CTTTCTAATGATGACAGCGATACC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRKACB (5567)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471379
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5567 PRKACB protein kinase cAMP-activat... NM_207578.3 99.7% 37.3% 276_277insA;763A>C
2 human 5567 PRKACB protein kinase cAMP-activat... XM_017001713.2 99.4% 37.2% 276_277insA;763_765delAAG
3 human 5567 PRKACB protein kinase cAMP-activat... XM_017001717.1 95.3% 33% (many diffs)
4 human 5567 PRKACB protein kinase cAMP-activat... NM_001300917.2 94.4% 31.1% (many diffs)
5 human 5567 PRKACB protein kinase cAMP-activat... XM_017001716.1 94.3% 33.4% (many diffs)
6 human 5567 PRKACB protein kinase cAMP-activat... XM_017001720.1 94.3% 31.9% (many diffs)
7 human 5567 PRKACB protein kinase cAMP-activat... XM_017001715.1 94.2% 32.4% (many diffs)
8 human 5567 PRKACB protein kinase cAMP-activat... XM_017001718.2 94.2% 31% (many diffs)
9 human 5567 PRKACB protein kinase cAMP-activat... XM_017001719.1 94% 31.7% (many diffs)
10 human 5567 PRKACB protein kinase cAMP-activat... XM_017001714.1 94% 32.3% (many diffs)
11 human 5567 PRKACB protein kinase cAMP-activat... XM_017001711.1 93.9% 32.8% (many diffs)
12 human 5567 PRKACB protein kinase cAMP-activat... XM_017001709.1 93.7% 32.6% (many diffs)
13 human 5567 PRKACB protein kinase cAMP-activat... XM_017001712.2 93.7% 32.9% (many diffs)
14 human 5567 PRKACB protein kinase cAMP-activat... XM_017001710.2 93.4% 32.8% (many diffs)
15 human 5567 PRKACB protein kinase cAMP-activat... XM_017001707.1 92.9% 32.1% (many diffs)
16 human 5567 PRKACB protein kinase cAMP-activat... XM_017001706.1 92.6% 32% (many diffs)
17 human 5567 PRKACB protein kinase cAMP-activat... XM_017001708.2 92.6% 32.3% (many diffs)
18 human 5567 PRKACB protein kinase cAMP-activat... NM_001300915.2 92.4% 32.1% (many diffs)
19 human 5567 PRKACB protein kinase cAMP-activat... NM_001300916.2 82.2% 27.9% (many diffs)
20 human 5567 PRKACB protein kinase cAMP-activat... XM_005271020.2 82.1% 27.8% (many diffs)
21 human 5567 PRKACB protein kinase cAMP-activat... NM_002731.3 72.8% 27.3% (many diffs)
22 human 5567 PRKACB protein kinase cAMP-activat... XM_005271018.4 69.7% 24.2% (many diffs)
23 human 5567 PRKACB protein kinase cAMP-activat... XM_005271017.3 69.2% 24.5% (many diffs)
24 human 5567 PRKACB protein kinase cAMP-activat... XM_005271016.1 69.1% 23.7% (many diffs)
25 human 5567 PRKACB protein kinase cAMP-activat... NM_001242859.2 69.1% 24.1% (many diffs)
26 human 5567 PRKACB protein kinase cAMP-activat... XM_006710758.2 69% 24.2% (many diffs)
27 human 5567 PRKACB protein kinase cAMP-activat... NM_001242858.2 69% 22.7% (many diffs)
28 human 5567 PRKACB protein kinase cAMP-activat... XM_005271019.1 68.9% 23.3% (many diffs)
29 human 5567 PRKACB protein kinase cAMP-activat... XM_005271015.3 68.6% 23.9% (many diffs)
30 human 5567 PRKACB protein kinase cAMP-activat... NM_001242857.2 68.6% 23.7% (many diffs)
31 human 5567 PRKACB protein kinase cAMP-activat... NM_001242860.2 68.4% 23.8% (many diffs)
32 human 5567 PRKACB protein kinase cAMP-activat... NM_001242862.2 66.2% 18.2% (many diffs)
33 human 5567 PRKACB protein kinase cAMP-activat... NM_182948.4 62.7% 21.3% (many diffs)
34 human 5567 PRKACB protein kinase cAMP-activat... NM_001242861.2 59.8% 21.2% (many diffs)
35 mouse 18749 Prkacb protein kinase, cAMP depend... NM_011100.4 63.3% 25.9% (many diffs)
36 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164198.1 59.7% 22.2% (many diffs)
37 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164199.1 59.6% 21.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
399
ORF length:
333
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gaacgcggcg accgccaaga aaggcagcga ggtggagagc gtgaaagagt
121ttctagccaa agccaaagaa gactttttga aaaaatggga gaatccaact cagaataatg
181ccggacttga agattttgaa aggaaaaaaa cccttggaac aggttcattt ggaagagtca
241tgttggtaaa acacaaagcc actgaaCAGT ATTATGCCAT GAAGATCTTA GATAAGCAGA
301AGGTTGTTAA ACTGAAGCAA ATAGAGCATA CTTTGAATGA GAAAAAGAAT ATTACAGGCA
361GTGAATTTTC CTTTCCTTGT TCGACTGGAG TATGCTTTTA AGGATAATTC TAATTTATAC
421ATGGTTATGG AATATGTCCC TGGGGGTGAA ATGTTTTCAC ATCTAAGAAG AATTGGAAGG
481TTCAGTGAGC CCCATGCACG GTTCTATGCA GCTCAGATAG TGCTAACATT CGAGTACCTC
541CATTCACTAG ACCTCATCTA CAGAGATCTA AAACCTGAAA ATCTCTTAAT TGACCATCAA
601GGCTATATCC AGGTCACAGA CTTTGGGTTT GCCAAAAGAG TTAAAGGCAG AACTTGGACA
661TTATGTGGAA CTCCAGAGTA TTTGGCTCCA GAAATAATTC TCAGCAAGGG CTACAATAAG
721GCAGTGGATT GGTGGGCATT AGGAGTGCTA ATCTATGAAA TGGCAGCTGG CTATCCCCCA
781TTCTTTGCAG ACCAACCAAT TCAGATTTAT GAAAAGATTG TTTCTGGACA GAACTTTTGC
841CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT
901CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT
961TATATATCTT GTGGAAAGGA CGACTTTCTA ATGATGACAG CGATACCACG CGTTAAGTCg
1021acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)