Construct: ORF TRCN0000471401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009055.1_s317c1
- Derived from:
- ccsbBroadEn_13068
- DNA Barcode:
- CTCGCCAAACAATTCTACCCGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C10orf71 (118461)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118461 | C10orf71 | chromosome 10 open reading ... | NM_001135196.2 | 49.2% | 47.9% | (many diffs) |
| 2 | human | 118461 | C10orf71 | chromosome 10 open reading ... | XM_005269476.4 | 49.2% | 47.9% | (many diffs) |
| 3 | human | 118461 | C10orf71 | chromosome 10 open reading ... | XM_005269477.3 | 49.2% | 47.9% | (many diffs) |
| 4 | human | 118461 | C10orf71 | chromosome 10 open reading ... | XM_005269478.4 | 49.2% | 47.9% | (many diffs) |
| 5 | human | 118461 | C10orf71 | chromosome 10 open reading ... | XM_017015619.1 | 38.8% | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2202
- ORF length:
- 2133
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgcaagga aataagaagt gcacagacgc gttcagcgac tcctccagca 121 tcggcagcgt gttggatgat gcagacaggg aggtgagcag cctaacagac cgggcattcc 181 ggagtttgtg catctccgag gacacatcct tccatgactc ctatctggct gtgtccccgg 241 atatcacccg acaggtgttt gggacttttc accagagaac agtgggccac acccagagga 301 aaagtggcat ttggagccag ttaccgtcac agggcacgga acattcgggc tgggcggcca 361 ccttccaaca gctacccaag tacgttcagg gagaggaaaa gtaccccaaa accagccccc 421 caccaacgcc agtccagagg agactggagg tgccagtttc cggcctaagg agcagcaata 481 agcctgtctc caaagtatca acactaatta aatctttcga caggaccgag agccaacgtt 541 gtgagagcag gcccactgcc agcaagcctc cggctctgaa aaatcctccc aaattcgctc 601 ctcttccaga aaacagtgtc aacttctgct tcgattctgc ctttctgaca gtcaggaggg 661 tgcccgctga agtttccaac acccatcaga acagctacca gccaggcagg aagcacggag 721 aacaggagtc ctccaagaat ccagaaatgg cctgtcacgg ctccagcagc ttcctcccag 781 cagccaatga cacggccacc ttatgtgagt caaagttccc ctctccacac cacaagccag 841 tcacgggtga gcctgggaga ggcaaaggta cctttctgca cagtgaaaat agtgcttttg 901 agtcatggaa tgcccaccaa ccaaagctgc tggagagaaa ggacacagct ggaaccgtcc 961 cagaaagcaa agctcccaag cactatgggg acacgacctt gctaagagaa ccctgtcctc 1021 ctgagctcac agtctctccc tgccaggtcc aggccagctg cagtcaggaa gagaacagac 1081 tcgaagcagg ggctctgtcc acatctatac cctgggggtg cagggatcca ggagcccagg 1141 tatttgctgt ggaaggaaaa gctcccagct cacaacctga ttctcaagag aagccagccc 1201 agcccccatg gaggaagcca aagactggca aaaaagggaa agaaagtcta caagatactt 1261 tagaagaaaa gacacagacc aaccagagag gcccaccttt gtatacaaaa cacaaccccc 1321 aggaacagtt ttcagaaaac aatgctcttg acctgcctgt ggaacccaat gaacattatg 1381 atcccccctt taacatcagt aagctcctga cccccatcat acccagcaag cacgccctgg 1441 attcagcagc cagccagcca gcagagcgaa ccccatcacc cccaggacag ctaaacggat 1501 accaagagaa ggagcccagt gaatgtcagt ctcgagacag ctacaagtcc aaagccccta 1561 gcctgctgtt caacctcaag gacgtgcgga agcgtgttaa gagcacatac agttcctcac 1621 ctctcttgaa agtgcttgat gagaaaacta gaggtaaggt tgatggaaag caagaacctg 1681 tgagcaacgg tgtcatcctc cccaatgggc ttgaggaaag ccctccaaat gagctttcta 1741 aggagagacc cgctgatgac cccactgcat cacacatcaa tccccagaag gaccctacag 1801 ctgaccccag tgagccctct gcagacagct atctaactct tagcacagct ccgactatcg 1861 ccaaagcccc cttctatgtc aatggggagg ctgctgagag aagcagttat gagaacaagg 1921 aggtggaagg agagttGGAG ATGGGTCCTG CCGGATCCAG CTGGTGTCCA GACTCCAGGG 1981 AACACCGCCC CAGGAAACAC CTCTCCCTGA GGCTTTGCAA TAGGGATCCT GAGCCTGGAG 2041 GGGCTACAGA GAAAATGAAG ACCCACCAGC TAGAGAATGG GCTCTCCAGA TCTGTGTCCC 2101 AAGAGACAGA ACCTGAGAGG GAAGCAGGAC TTCAGAACAC ACACACACAC GATCATCAAC 2161 ACATACTTAG CCTTTTTAGA TCCATAAAGT CCAGAAGGCA GTTGCCAACT TTCTTGTACA 2221 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2281 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2341 AGGACGACTC GCCAAACAAT TCTACCCGCG GACGCGTTAA GTCgacaatc aacctctgga 2401 ttacaaaatt tgtgaaagat t