Construct: ORF TRCN0000471538
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015098.1_s317c1
- Derived from:
- ccsbBroadEn_12622
- DNA Barcode:
- AAGCAACTATGAACTGCCATCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTCD (79807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471538
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79807 | GSTCD | glutathione S-transferase C... | NM_001031720.3 | 99.8% | 99.8% | 989_991delCAG |
| 2 | human | 79807 | GSTCD | glutathione S-transferase C... | NM_001370181.1 | 99.8% | 99.8% | 989_991delCAG |
| 3 | human | 79807 | GSTCD | glutathione S-transferase C... | XM_011532248.3 | 99.8% | 99.8% | 989_991delCAG |
| 4 | human | 79807 | GSTCD | glutathione S-transferase C... | XM_011532249.3 | 99.8% | 99.8% | 989_991delCAG |
| 5 | human | 79807 | GSTCD | glutathione S-transferase C... | XM_011532252.3 | 99.8% | 99.8% | 989_991delCAG |
| 6 | human | 79807 | GSTCD | glutathione S-transferase C... | NM_024751.3 | 86% | 86% | 165_166ins261;728_730delCAG |
| 7 | human | 79807 | GSTCD | glutathione S-transferase C... | XR_938771.1 | 71.3% | (many diffs) | |
| 8 | mouse | 67553 | Gstcd | glutathione S-transferase, ... | NM_026231.2 | 84% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1962
- ORF length:
- 1896
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agccataaag aaaagtctta cagaagaaga atacctgtac ctggactttt 121 ctcaccaaac agaaggatgc atctttcctc ttcatacatc tgtaacttta tttctgttat 181 cttactgtga ctgtaaaatc tttaaaattt gcttagttgt caccaaagag gtgagtagag 241 atagttcact actaagagat gacctgatcc aggatgttga aatacagatt atttcaaggc 301 aggagctccc accaatagtc caaaattgct gtttgcctgc agtagtagaa cgatcagaca 361 atttttgtag agcaggactt gctgttgtat tgagacacat aatccagaaa tcctatgaag 421 cagacccctt aaagaaggaa cttttggaac ttctgggctt taaaaagact tgcttgaaag 481 cctgtgctga agttagtcag tggaccaggc tatgtgaact caccatccct ttggctattg 541 agaattttct cagagaatct tctgaccagc ccccaactat acctgtagaa atactacagc 601 tagagaaaaa gcttagtgag cctgttagag tgcataatga tgataaactc cgcaggcaga 661 agctcaagca acagaaggct gatggagttg ggcctcccct tactaaggga aaggcaaaga 721 gcaaggtcca cacacaggaa acatctgaag ggttggattc ttcatccaag agtctggaac 781 tgaaagtggc attctcaaag ctcacagtac aggaagaacc agctactacc aacagagagc 841 cttctcacat cagaaaagca aaagcctccg accttccacc tctggagcat gtgtttgcag 901 aagggcttta cttcactctg gcagatattg tgctcttgcc ctgtatccat catttcttgg 961 taattatcag caggaaattt tctgagaagc tggtagaatt tccattgcta gcctcttggt 1021 accagaggat tcaggaagtg ccaggagtaa aaacagcttc taagtgtggg atccaatttc 1081 tccatttacc aaagttgttg acaacctcaa ctgaacagca tccaaactta tgtgaagtcc 1141 caggtgtaga agagcaaagc gatcctttat ttataggagg accaagacca accatggcca 1201 agttaatgga aaagggcatt gaagtgatgt tttctcccca cccttgccct acttggactc 1261 ttgattggaa tgttctccct gcagcagtca gcccaaagga aggtaaaatg tccagtgatc 1321 gagctttgag gaagcagcaa cagttgaaca accttgtcta tgtggtaaca aatcaggcca 1381 aacctggtga cagaattgtg gatttctgca gcggtggggg ccatgttgga attgtccttg 1441 ctcacatgct gccatcatgt caggttacat taatagaaaa caaggaatta tcatTAATTC 1501 GTGCTAAGAA GAGAAGTGAT GAACTGGGTT TAAGCAACAT TTGGTTCATT CAAGCAAATA 1561 TGGAATATTT TACTGGGATG TTTAATATTG GAGTAGCATT GCATGCTTGT GGAGTGGCAA 1621 CAGACATGGT GATTGAGCAC TGTATCAAAA CACGGGCTTC CTTCGTCACA TGCCCTTGCT 1681 GTTATGGTTT CATTCAGAAC ACCTCAAAGT TCAATTTTCC AAAAAGTGAA CAATTCAAGA 1741 AAACTTTATC ATACAAGGAA CACATGATTC TGTGCAGATT TGCAGACCAG ACAGCTGTCC 1801 AGCTCCCACC CCAACGAAGG CTCATAGGAA AACAGTGCAT GTGCTTGGTG GATCTGGATC 1861 GAGCAAGAGC TGCAGAAGAA TGTGGATACT CCGTTCAAGT GATATCCATG GAGCCAGAGA 1921 GCTGCTCTCC CAAAAATAAC ATGATTGTGG GAGTCCCCAT TTACCCAACT TTCTTGTACA 1981 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2041 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2101 AGGACGAAAG CAACTATGAA CTGCCATCCG AACGCGTTAA GTCgacaatc aacctctgga 2161 ttacaaaatt tgtgaaagat t