Construct: ORF TRCN0000471582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014058.1_s317c1
- Derived from:
- ccsbBroadEn_05709
- DNA Barcode:
- GGATCAACTACTTCAAAAATGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOT1 (641371)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471582
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | NM_001037161.2 | 100% | 100% | |
| 2 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_006821.5 | 86.2% | 86.1% | (many diffs) |
| 3 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021590.1 | 67.7% | 65.6% | (many diffs) |
| 4 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021589.1 | 67.5% | 63.9% | (many diffs) |
| 5 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_001364177.1 | 66.5% | 64.6% | (many diffs) |
| 6 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021591.1 | 61.5% | 61.5% | 0_1ins486 |
| 7 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021592.1 | 61.5% | 61.5% | 0_1ins486 |
| 8 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NR_046028.1 | 60% | (many diffs) | |
| 9 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_001364178.1 | 48.2% | 45.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1332
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgacg ctgatcctgg agcccgcggg ccgctgctgc tgggacgaac 121 cggtgcgaat cgccgtgcgc ggcctagccc cggagcagcc ggtcacgctg cgcgcgtccc 181 tgcgcgacga gaagggcgcg cttttccagg cccacgcgcg ctaccgcgcc gacacccttg 241 gcgagctgga cctggagcgc gcgcccgcgc tgggcggcag cttcgcgggg cttgagccca 301 tggggctgct ctgggccttg gagcccgaga aacccttggt gcggctggtg aagcgcgacg 361 tgcgaacgcc cttggccgtg gagctggagg tgctggatgg ccacgacccc gaccccgggc 421 ggctgctgtg ccgggtgcgg cacgagcgct acttcctccc gcccggggtg cggcgcgagc 481 cggtgcgcgc gggccgggtg cgaggcacgc tcttcctgcc gccagaacct gggccctttc 541 ctggcattgt ggacatgttc ggaactggag gtggcctgct ggagtatcgg gctagtctgc 601 tggctgggaa gggttttgct gtgatggctc tggcttacta taactatgaa gacctcccca 661 agaccatgga gacgctccat ctggagtact ttgaagaagc tgtgaactac ttgctcagtc 721 atcctgaggt aaaaggtcca ggagttgggc tgcttggaat ttccaaaggg ggtgagctct 781 gcctttccat ggcctctttc ctgaagggca tcacggctgc tgtcgtcatc aacggctctg 841 tggccaatgt tgggggaacc ttacgcTACA AGGGCGAGAC CCTGCCCCCT GTGGGCGTCA 901 ACAGAAATCG CATCAAGGTG ACCAAAGATG GCTATGCAGA CATTGTGGAT GTCCTGAACA 961 GCCCTTTGGA AGGACCTGAC CAGAAGAGCT TCATTCCTGT GGAAAGGGCA GAGAGCACCT 1021 TCCTGTTCCT GGTAGGTCAG GATGACCACA ACTGGAAGAG TGAGTTCTAT GCTAATGAGG 1081 CCTGTAAACG CTTGCAGGCC CATGGGAGGA GAAAGCCCCA GATCATCTGT TACCCAGAGA 1141 CAGGGCACTA TATTGAGCCT CCTTACTTCC CCCTGTGTCG GGCTTCCCTG CATGCCTTGG 1201 TGGGCAGTCC TATTATCTGG GGAGGGGAGC CCAGGGCTCA TGCCATGGCT CAGGTGGATG 1261 CTTGGAAACA ACTCCAGACT TTCTTCCACA AACACTTGGG TGGCCACGAG GGGACAATCC 1321 CATCAAAAGT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGA TCAACTACTT CAAAAATGGG 1501 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t