Construct: ORF TRCN0000471594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009723.1_s317c1
- Derived from:
- ccsbBroadEn_00106
- DNA Barcode:
- GGTGCGTGGTTTCTTTATATCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARRB1 (408)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471594
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 408 | ARRB1 | arrestin beta 1 | NM_004041.5 | 100% | 100% | |
| 2 | human | 408 | ARRB1 | arrestin beta 1 | NM_020251.4 | 98% | 98% | 997_998ins24 |
| 3 | human | 408 | ARRB1 | arrestin beta 1 | XM_017017752.2 | 94.5% | 94.5% | 1146_1217del |
| 4 | human | 408 | ARRB1 | arrestin beta 1 | XM_017017753.1 | 93.4% | 93.4% | 4_5insGCGACAAAGGGACCC;1131_1202del |
| 5 | human | 408 | ARRB1 | arrestin beta 1 | XM_011545034.2 | 92.8% | 90.9% | (many diffs) |
| 6 | human | 408 | ARRB1 | arrestin beta 1 | XM_017017754.2 | 92.7% | 92.7% | 997_998ins24;1122_1193del |
| 7 | human | 408 | ARRB1 | arrestin beta 1 | XM_011545035.2 | 91% | 89.1% | (many diffs) |
| 8 | human | 408 | ARRB1 | arrestin beta 1 | XM_017017750.1 | 88.1% | 86.3% | (many diffs) |
| 9 | human | 408 | ARRB1 | arrestin beta 1 | XM_017017751.1 | 86.4% | 84.6% | (many diffs) |
| 10 | mouse | 109689 | Arrb1 | arrestin, beta 1 | NM_177231.2 | 89.7% | 98.5% | (many diffs) |
| 11 | mouse | 109689 | Arrb1 | arrestin, beta 1 | XM_006507200.2 | 88.6% | 97.3% | (many diffs) |
| 12 | mouse | 109689 | Arrb1 | arrestin, beta 1 | NM_178220.3 | 88% | 96.6% | (many diffs) |
| 13 | mouse | 109689 | Arrb1 | arrestin, beta 1 | XM_011241644.2 | 86.4% | 94.1% | (many diffs) |
| 14 | mouse | 109689 | Arrb1 | arrestin, beta 1 | XM_006507198.3 | 82% | 88.6% | (many diffs) |
| 15 | mouse | 109689 | Arrb1 | arrestin, beta 1 | XM_006507199.3 | 80.3% | 86.9% | (many diffs) |
| 16 | mouse | 109689 | Arrb1 | arrestin, beta 1 | XR_378207.3 | 58.4% | (many diffs) | |
| 17 | mouse | 109689 | Arrb1 | arrestin, beta 1 | XR_378208.3 | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgacaaaggg acccgagtgt tcaagaaggc cagtccaaat ggaaagctca 121 ccgtctacct gggaaagcgg gactttgtgg accacatcga cctcgtggac cctgtggatg 181 gtgtggtcct ggtggatcct gagtatctca aagagcggag agtctatgtg acgctgacct 241 gcgccttccg ctatggccgg gaggacctgg atgtcctggg cctgaccttt cgcaaggacc 301 tgtttgtggc caacgtacag tcgttcccac cggcccccga ggacaagaag cccctgacgc 361 ggctgcagga acgcctcatc aagaagctgg gcgagcacgc ttaccctttc acctttgaga 421 tccctccaaa ccttccatgt tctgtgacac tgcagccggg gcccgaagac acggggaagg 481 cttgcggtgt ggactatgaa gtcaaagcct tctgcgcgga gaatttggag gagaagatcc 541 acaagcggaa ttctgtgcgt ctggtcatcc ggaaggttca gtatgcccca gagaggcctg 601 gcccccagcc cacagccgag accaccaggc agttcctcat gtcggacaag cccttgcacc 661 tagaagcctc tctggataag gagatctatt accatggaga acccatcagc gtcaacgtcc 721 acgtcaccaa caacaccaac aagacggtga agaagatcaa gatctcagtg cgccagtatg 781 cagacatctg ccttttcaac acagctcagt acaagtgccc tgttgccatg gaagaggctg 841 atgacactgt ggcacccagc tcgacgttct gcaaggtcta cacactgacc cccttcctag 901 ccaataaccg agagaagcgg ggcctcgcct tggacgggaa gctcaagcac gaAGACACGA 961 ACTTGGCCTC TAGCACCCTG TTGAGGGAAG GTGCCAACCG TGAGATCCTG GGGATCATTG 1021 TTTCCTACAA AGTGAAAGTG AAGCTGGTGG TGTCTCGGGG CGGCCTGTTG GGAGATCTTG 1081 CATCCAGCGA CGTGGCCGTG GAACTGCCCT TCACCCTAAT GCACCCCAAG CCCAAAGAGG 1141 AACCCCCGCA TCGGGAAGTT CCAGAGAACG AGACGCCAGT AGATACCAAT CTCATAGAAC 1201 TTGACACAAA TGATGACGAC ATTGTATTTG AGGACTTTGC TCGCCAGAGA CTGAAAGGCA 1261 TGAAGGATGA CAAGGAGGAA GAGGAGGATG GTACCGGCTC TCCACAGCTC AACAACAGAT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AGTATTTCGA TTTCTTGGCT 1441 TTATATATCT TGTGGAAAGG ACGAGGTGCG TGGTTTCTTT ATATCATAAC GCGTTAAGTC 1501 gacaatcaac ctctggatta caaaatttgt gaaagatt