Construct: ORF TRCN0000471652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007378.4_s317c1
- Derived from:
- ccsbBroadEn_15252
- DNA Barcode:
- TCGAAGAAAGCGAATCCCTTGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNCK (139728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471652
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_024452339.1 | 28.5% | 25.7% | (many diffs) |
| 2 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366975.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 3 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366976.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 4 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366977.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 5 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366978.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 6 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366979.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 7 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001366980.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 8 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_006724809.3 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 9 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531107.2 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 10 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531111.2 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 11 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531112.2 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 12 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_017029279.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 13 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_024452340.1 | 24.7% | 24.7% | 68_69ins87;277_1029del |
| 14 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001135740.1 | 23.6% | 23.6% | 1_51del;119_120ins87;328_1080del |
| 15 | human | 139728 | PNCK | pregnancy up-regulated nonu... | XM_011531108.2 | 23.5% | 23.5% | 1_54del;122_123ins87;331_1083del |
| 16 | human | 139728 | PNCK | pregnancy up-regulated nonu... | NM_001039582.3 | 20.2% | 20.2% | 1_249del;317_318ins87;526_1278del |
| 17 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_001199351.1 | 21.5% | 23.9% | (many diffs) |
| 18 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_001199352.1 | 21.5% | 23.9% | (many diffs) |
| 19 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | NM_012040.3 | 21.5% | 23.9% | (many diffs) |
| 20 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_006528344.4 | 21.5% | 23.9% | (many diffs) |
| 21 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_011247692.3 | 21.5% | 23.9% | (many diffs) |
| 22 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_030251530.1 | 21.5% | 23.9% | (many diffs) |
| 23 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XM_030251531.1 | 21.5% | 23.9% | (many diffs) |
| 24 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953034.1 | 16% | (many diffs) | |
| 25 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_001782761.2 | 15.5% | (many diffs) | |
| 26 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953033.1 | 14% | (many diffs) | |
| 27 | mouse | 93843 | Pnck | pregnancy upregulated non-u... | XR_003953032.1 | 13.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctgctgaag aaacacacgg aggacatcag cagcgtctac gagatccgcg 121 agaggctcgg ctcggggccc tccccgctcc actctctctc ccTCCTGCCT CTCCTCTCTT 181 CCCACTTCCT TCCCACCTCC CACCGCCCCG TCTGCGGCAG GGGTGCCTTC TCCGAGGTGG 241 TGCTGGCCCA GGAGCGGGGC TCCGCACACC TCGTGGCCCT CAAGTGCATC CCCAAGAAGG 301 CCCTCCGGGG CAAGGAGGCC CTGGTGGAGA ACGAGATCGC AGTGCTCCGT AGGATCAGTC 361 ACCCCAACAT CGTCGCTCTG GAGGATGTCC ACGAGAGCCC TTCCCACCTC TACCTGGCCA 421 TGGAACTGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCGAAG AAAGCGAATC CCTTGCAGAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt