Construct: ORF TRCN0000471710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016641.1_s317c1
- Derived from:
- ccsbBroadEn_00610
- DNA Barcode:
- CGGCTTATCACCGACTTTCCCGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAGE5 (2577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2577 | GAGE5 | G antigen 5 | NM_001475.1 | 100% | 100% | |
| 2 | human | 2576 | GAGE4 | G antigen 4 | NM_001474.1 | 99.7% | 99.1% | 67A>G |
| 3 | human | 2578 | GAGE6 | G antigen 6 | NM_001476.1 | 99.7% | 99.1% | 289G>A |
| 4 | human | 100008586 | GAGE12F | G antigen 12F | NM_001098405.2 | 99.4% | 98.2% | 67A>G;223C>G |
| 5 | human | 645073 | GAGE12G | G antigen 12G | NM_001098409.2 | 99.4% | 98.2% | 67A>G;223C>G |
| 6 | human | 729428 | GAGE12B | G antigen 12B | NM_001127345.2 | 99.4% | 98.2% | 67A>G;223C>G |
| 7 | human | 26748 | GAGE12I | G antigen 12I | NM_001477.1 | 99.4% | 98.2% | 67A>G;223C>G |
| 8 | human | 2579 | GAGE7 | G antigen 7 | NM_021123.3 | 99.4% | 98.2% | 67A>G;223C>G |
| 9 | human | 2543 | GAGE1 | G antigen 1 | NM_001040663.2 | 99.1% | 98.2% | 67A>G;337_338delGGinsAA |
| 10 | human | 729396 | GAGE12J | G antigen 12J | NM_001098406.2 | 99.1% | 98.2% | 45A>G;47C>G;67A>G |
| 11 | human | 729422 | GAGE12C | G antigen 12C | NM_001098408.1 | 99.1% | 97.4% | 67A>G;148T>C;223C>G |
| 12 | human | 729431 | GAGE12E | G antigen 12E | NM_001098418.1 | 99.1% | 97.4% | 67A>G;148T>C;223C>G |
| 13 | human | 100132399 | GAGE12D | G antigen 12D | NM_001127199.1 | 99.1% | 97.4% | 67A>G;148T>C;223C>G |
| 14 | human | 645051 | GAGE13 | G antigen 13 | NM_001098412.2 | 98.8% | 98.2% | (many diffs) |
| 15 | human | 729442 | GAGE12H | G antigen 12H | NM_001098410.1 | 98.8% | 96.5% | (many diffs) |
| 16 | human | 729408 | GAGE2D | G antigen 2D | NM_001098407.1 | 97.7% | 96.5% | (many diffs) |
| 17 | human | 26749 | GAGE2E | G antigen 2E | NM_001127200.3 | 97.7% | 96.5% | (many diffs) |
| 18 | human | 100101629 | GAGE8 | G antigen 8 | NM_012196.1 | 97.7% | 96.5% | (many diffs) |
| 19 | human | 645037 | GAGE2B | G antigen 2B | NM_001098411.3 | 97.4% | 95.7% | (many diffs) |
| 20 | human | 2574 | GAGE2C | G antigen 2C | NM_001472.2 | 97.4% | 95.7% | (many diffs) |
| 21 | human | 729447 | GAGE2A | G antigen 2A | NM_001127212.1 | 97.1% | 94.8% | (many diffs) |
| 22 | human | 102724473 | GAGE10 | G antigen 10 | NM_001098413.3 | 95.1% | 90.5% | (many diffs) |
| 23 | human | 2543 | GAGE1 | G antigen 1 | XM_011543896.2 | 90% | 89.5% | 67A>G;333_334insGAAAAGCAATCACAG;337_357del |
| 24 | human | 102724473 | GAGE10 | G antigen 10 | XM_024452325.1 | 82.3% | 73.5% | (many diffs) |
| 25 | human | 645073 | GAGE12G | G antigen 12G | NM_001354422.1 | 63.8% | 63.2% | 67A>G;202_203ins126 |
| 26 | human | 2543 | GAGE1 | G antigen 1 | NR_102272.1 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttggcgagga agatcgacct attattggcc TAGACCAAGG CGCTATGTAC 121 AGCCTCCTGA AGTGATTGGG CCTATGCGGC CCGAGCAGTT CAGTGATGAA GTGGAACCAG 181 CAACACCTGA AGAAGGGGAA CCAGCAACTC AACGTCAGGA TCCTGCAGCT GCTCAGGAGG 241 GAGAGGATGA GGGAGCATCT GCAGGTCAAG GGCCGAAGCC TGAAGCTGAT AGCCAGGAAC 301 AGGGTCACCC ACAGACTGGG TGTGAGTGTG AAGATGGTCC TGATGGGCAG GAGATGGACC 361 CGCCAAATCC AGAGGAGGTG AAAACGCCTG AAGAAGGTGA AAAGCAATCA CAGTGTTACC 421 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 481 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 541 TATATATCTT GTGGAAAGGA CGACGGCTTA TCACCGACTT TCCCGATACG CGTTAAGTCg 601 acaatcaacc tctggattac aaaatttgtg aaagatt