Construct: ORF TRCN0000471723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002410.1_s317c1
- Derived from:
- ccsbBroadEn_15717
- DNA Barcode:
- AACCCAGACTCCGGGTGATACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PITRM1 (10531)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_014889.4 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001242307.1 | 99.6% | 99.4% | (many diffs) |
| 3 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347729.1 | 98.3% | 97.6% | (many diffs) |
| 4 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347725.2 | 93.3% | 93.1% | (many diffs) |
| 5 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347730.1 | 92% | 91.3% | (many diffs) |
| 6 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001242309.1 | 89.8% | 88.5% | (many diffs) |
| 7 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747001.2 | 89% | (many diffs) | |
| 8 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144638.2 | 84.8% | (many diffs) | |
| 9 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144641.2 | 84.7% | (many diffs) | |
| 10 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144640.2 | 83.8% | (many diffs) | |
| 11 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144639.2 | 83.7% | (many diffs) | |
| 12 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747002.2 | 83.4% | (many diffs) | |
| 13 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747003.2 | 83.3% | (many diffs) | |
| 14 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747000.1 | 82.6% | (many diffs) | |
| 15 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347727.2 | 80% | 79.8% | (many diffs) |
| 16 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001746999.2 | 78.7% | (many diffs) | |
| 17 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747004.2 | 77.2% | (many diffs) | |
| 18 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347726.2 | 73.6% | 73.4% | (many diffs) |
| 19 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347728.2 | 57.9% | 57.8% | (many diffs) |
| 20 | mouse | 69617 | Pitrm1 | pitrilysin metallepetidase 1 | NM_145131.1 | 81.6% | 86.5% | (many diffs) |
| 21 | mouse | 69617 | Pitrm1 | pitrilysin metallepetidase 1 | NM_001360106.1 | 81.6% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3177
- ORF length:
- 3111
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcgctgcggc gggcggcggg gcctgtgtgt gctgaggcgg ctgagcggcg 121 gacatgcaca ccacagagcg tggcgatgga acagtaaccg ggcttgtgag agggctctgc 181 agtataaact aggagacaag atccatggat tcaccgtaaa ccaggtgaca tctgttcccg 241 agctgttcct gactgcagtg aagctcaccc atgatgacac aggagccagg tatttacacc 301 tggccagaga agacacgaat aatctgttca gcgtgcagtt ccgtaccact cccatggaca 361 gtactggtgt tcctcacatt cttgagcata ccgtcctttg tgggtctcag aaatatccgt 421 gcagagaccc tttcttcaaa atgttgaacc ggtccctctc cacgttcatg aacgccttca 481 cagctagtga ttatactctg tatccatttt ccacacaaaa tcccaaggac tttcagaatc 541 tcctctcggt gtatttggat gccacctttt tcccatgttt acgcgagctg gatttctggc 601 aggaaggatg gcggctggaa catgagaatc cgagcgaccc ccagacgccc ttggtcttta 661 aaggagtcgt ctttaatgag atgaagggag cgtttacaga caatgagagg atattctccc 721 agcaccttca gaacagactt cttcccgacc acacgtactc agtggtctcc gggggtgacc 781 cactgtgcat cccggagctt acatgggagc agcttaagca gtttcatgcc actcactatc 841 acccaagcaa tgctaggttc ttcacgtacg gtaattttcc attagaacag catctgaaac 901 aaattcacga ggaagcactg agcaaattcc agaaaattga accaagcacc gtggtgccag 961 ctcagacacc ctgggacaag cctagggaat tccagataac atgtggcccg gattcatttg 1021 ctacagatcc ctctaaacaa acaaccgtca gcgttagctt cctcttaccg gacatcaccg 1081 acacatttga agccttcaca ttaagtcttc tgtcttcact cttgacttct gggcccaatt 1141 ctccctttta caaagccttg attgaatctg gccttggcac agacttttct cctgatgttg 1201 gatataatgg ctacacgagg gaggcctact ttagtgtcgg cctccaaggg attgtggaga 1261 aagacattga gaccgtcaga agcctcatag acagaacgat tgatgaagta gttgagaaag 1321 gatttgaaga tgatcgaatt gaggctttac ttcataaaat tgaaatacag atgaaacatc 1381 agtctaccag ctttgggctg atgctgacat catacatagc ttcttgctgg aaccatgatg 1441 gggaccctgt ggagctcttg aagttgggaa atcagttagc taaattcaga cagtgcctgc 1501 aggaaaatcc aaaatttttg caagaaaaag taaaacagta ttttaagaat aaccagcata 1561 agctgacttt atcgatgagg ccagatgaca agtatcacga gaagcaggca caggtggaag 1621 ccacgaagct caagcagaag gtcgaggctc tgtcccccgg agacaggcag cagatctacg 1681 agaaaggtct agaattacgg agtcaacaaa gcaaacctca agatgcctct tgtctgccag 1741 cgttgaaagt ttccgatatt gaacccacca tacctgtcac agagttggac gtggtcctga 1801 cagctggaga tatccctgtt cagtactgcg cccagcccac caatggcatg gtgtatttcc 1861 gggccttctc cagcctgaac acactccccg aggagctgag gccctatgtg cccctcttct 1921 gcagcgtcct caccaagctg ggctgcggcc ttcttgacta ccgggagcag gctcagcaga 1981 tagaattgaa gaccggaggg atgagtgctt ctccccacgt gctccccgac gactcacaca 2041 tggacaccta cgagcagggt gtgcttttct cctctctctg cctggatcga aacctgccag 2101 acatgatgca gctatggagt gaaatattta acaacccgtg ctttgaagaa gaggagcact 2161 tcaaggtgct ggtgaagatg accgcccagg agctcgccaa tggaattcct gactctgggc 2221 acctgtacgc atccatcagg gcaggccgga ccctcacgcc cgcaggggac ctgcaggaga 2281 ccttcagcgg gatggatcag gtgcggctga tgaagaggat tgcagaaatg acagatatca 2341 aacccatcct gaggaagctc ccacgtatca agaaacactt gttaaatggt gataatatga 2401 ggtgttcagt gaatgcgact cctcagcaga tgcctcagac agaaaaagcg gtcgaagact 2461 tccttagaag catcggtcgg agtaaaaagg aacggaggcc tgtgcgccca cacacggtcg 2521 agaaacctgt gcccagcagc tctggtggag atgcccacgt tccccatggc tcccaggtca 2581 ttaggaagct ggtcatggaa cccaccttca agccctggca gatgaagact cacttcctga 2641 tgcccttccc ggtgaattac gtgggtgaat gcatccgaac tgtcccctac acggacccag 2701 atcatgccag tcttaaaatc cttgcacgtt tgatgactgc caaattcttg catacagaaa 2761 ttcgagaaaa aggcggtgct tatggtggag gcgcaaaact cagccacaat gggattttca 2821 CCCTTTACTC TTACAGGGAC CCAAATACAA TAGAGACGCT CCAGTCTTTT GGGAAGGCTG 2881 TCGACTGGGC TAAGTCTGGA AAATTCACAC AGCAAGACAT CGACGAAGCC AAACTTTCTG 2941 TCTTCTCAAC CATAGATGCT CCTGTCGCTC CTTCAGACAA AGGAATGGAC CACTTCTTGT 3001 ACGGCCTCTC GGATGAGATG AAGCAGGCCC ACAGAGAGCA GCTCTTTGCT GTCAGCCACG 3061 ACAAGCTCCT GGCCGTGAGC GATAGGTACC TCGGCACTGG GAAGAGCACA CACGGCCTGG 3121 CCATCCTCGG ACCCGAGAAC CCGAAAATTG CCAAGGACCC ATCCTGGATC ATCCGATGCC 3181 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3241 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3301 ATATATCTTG TGGAAAGGAC GAAACCCAGA CTCCGGGTGA TACACAACGC GTTAAGTCga 3361 caatcaacct ctggattaca aaatttgtga aagatt