Construct: ORF TRCN0000471790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018910.3_s317c1
- Derived from:
- ccsbBroadEn_14944
- DNA Barcode:
- GGAAGCATCCTTCCGAGCCTACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ULK2 (9706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471790
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9706 | ULK2 | unc-51 like autophagy activ... | NM_001142610.2 | 99.3% | 73.9% | (many diffs) |
| 2 | human | 9706 | ULK2 | unc-51 like autophagy activ... | NM_014683.4 | 99.3% | 73.9% | (many diffs) |
| 3 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025424.2 | 97.3% | 72.4% | (many diffs) |
| 4 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025425.2 | 97.3% | 72.4% | (many diffs) |
| 5 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025426.2 | 94.8% | 69.9% | (many diffs) |
| 6 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_011524087.2 | 87.9% | 62.5% | (many diffs) |
| 7 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025427.2 | 74.4% | 50% | (many diffs) |
| 8 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XR_001752701.2 | 73.9% | (many diffs) | |
| 9 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025428.2 | 71.8% | 47.3% | (many diffs) |
| 10 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XR_001752700.2 | 49.8% | (many diffs) | |
| 11 | mouse | 29869 | Ulk2 | unc-51 like kinase 2 | NM_013881.4 | 89.9% | 68.7% | (many diffs) |
| 12 | mouse | 29869 | Ulk2 | unc-51 like kinase 2 | XM_006533508.3 | 88.4% | 67.8% | (many diffs) |
| 13 | mouse | 29869 | Ulk2 | unc-51 like kinase 2 | XM_006533509.3 | 84.4% | 63.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2478
- ORF length:
- 2409
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggtggtg ggtgacttcg agtacagcaa gagggatctc gtgggacacg 121 gggccttcgc cgtggtcttc cgggggcggc accgccagaa aactgattgg gaggtagcta 181 ttaaaagtat taataaaaag aacttgtcaa aatcacaaat actgcttgga aaggaaatta 241 aaatcttaaa ggaacttcag catgaaaata ttgtagcact ctatgatgtt caggaattac 301 ccaactctgt ctttttggtg atggagtatt gcaatggtgg agacctcgca gattatttgc 361 aagcgaaagg gactctcagt gaagacacga tcagagtgtt tctgcatcag attgctgctg 421 ccatgcgaat cctgcacagc aaaggaatca tccacagaga tctcaaacca cagaacatct 481 tgctgtccta tgccaatcgc agaaaatcaa gtgtcagtgg tattcgcatc aaaatagcgg 541 attttggttt tgctcgttac ctacatagta acatgatggc tgcaacactg tgtggatccc 601 cgatgtacat ggctcctgag gttattatgt ctcaacatta tgatgctaag gctgacttgt 661 ggagcatagg aacagtgata taccaatgcc tagttggaaa accacctttt caggccaata 721 gtcctcaaga cttaaagatg ttttatgaaa aaaacaggag cttaatgcct agtattccca 781 gagaaacatc accttatttg gctaatctct tttgggtttg cttcagagaa accaaaaaga 841 taagaatgga ctttgaagcg tttttagcca tcctttttct tgagcaaggt ccagtaaaaa 901 aatcttgccc agttccagtg cccatgtatt ctggttctgt ctctggaagc tcctgtggca 961 gctctccatc ttgtcgtttt gcttctccac catcccttcc agatatgcag catattcagg 1021 aagaaaactt atcttcccca ccattgggtc ctcccaacta tctacaagtt tccaaagatt 1081 ctgccagtac tagtagcaag aactcttctt gtgacacgga tgactttgtt ttggtgccac 1141 acaacatctc gtcagaccac tcatgtgata tgccaatggg gactgctggc agacgtgctt 1201 caaatgaatt cttggtgtgt ggagggcagt gtcagcctac tgtgtcacct cacagcgaaa 1261 cagcaccaat tccagttcct actcaaataa ggaattatca gcgcatagag cagaatctta 1321 catctactgc cagctcaggc acaaatgtac atggttctcc aagatctgca gtggtacgaa 1381 ggtccaacac cagccccatg ggcttcctcc ggccgggatc atgctcccca gtaccagcag 1441 acacagcaca gacagttgga cgaaggctct ccactgggtc ttctaggcct tactcacctt 1501 cccctttggt tggtaccatt cctgagcaat tcagtcagtg ctgctgtggg catcctcagg 1561 gccatgactc caggagtaga aactcctcag gttctccagt gccacaagct cagtccccac 1621 agtctctctt atcgggtgct agactgcaga gcgcccccac cctcactgac atctatcaga 1681 acaagcagaa gctcagaaaa cagcactctg accccgtgtg cccatcccat actggggctg 1741 ggtacagcta ctcgcctcag cccagtcggc ctggcagcct tggaacttct cccaccaagc 1801 acttggggtc ctctccacgg agttctgact ggttctttaa aactcctttg ccaacaatca 1861 ttggctctcc tactaagacc acagctcctt tcaaaatccc taaaactcaa gcatcttcca 1921 acctgttagc cttggttact cgtcatgggc ctgctgaaga acagtcgaaa gatgggaatg 1981 agccacggga atgtgcccat tgcctcttag tgcaaggaag tgagaggcag cgggccgagc 2041 agcagagcaa ggcagtgttt ggcagatctg tcagtaccgg gaagttatca gatcaacaag 2101 gaaagactcc tatatgtcga catcagggca gcacagacag tttaaataca gaacgaccaa 2161 tggatatagc tccggcagga gcctgtggtg gtgttctggc acctcctgca ggtacagcag 2221 caagttccaa ggctgtcctc ttcactgtag ggtctcctcc acacagtgcg gcagccccca 2281 cttgtaccca catgttcctt cgaacaagaa caacctcagt ggggcccagc aactccgggg 2341 gctctctttg tgccatgagt ggccgcgttt gcgtggggtc ccccgcttgc cccaagcctt 2401 cgggtctttc cctttccagg agcagaggca gctcccagcc tgagatacgt gccttacggt 2461 gcttcacccc cccagcctag agggctcatc acctttgaag cccctgaact gccggaggag 2521 acgctgatgg agcgggaaca cacagacacc ttacgccatc tgaatgtgat gctgatgttc 2581 actgagtgtg tgctggacct gacagccatg aggggaggaa accntgagct gtgcacatct 2641 gctgtgtcct tgtaccagat ccaggagagt gtggtggtgg accagatcag tcagctgagc 2701 aaagactggg ggcgggtgga gcagctggtg ttgtacatga aagcagcaca gctgcttgcg 2761 gcttctcTGC ATCTTGCCAA AGCCCAGATC AAGTCCGGGA AACTGAGCCC ATCCACAGCT 2821 GTGAAACAAG TTGTCAAGAA TCTGAACGAA CGATATAAAT TCTGCATCAC CATGTGCAAG 2881 AAACTTACAG AAAAGCTGAA TCGATTCTTC TCTGACAAAC AGAGGTTTAT TGATGAAATC 2941 AACAGTGTGA CTGCAGAGAA ACTCATCTAT AATTGTGCTG TAGAAATGGT TCAGTCTGCA 3001 GCCCTGGATG AGATGTTTCA GCAGACCGAA GATATTGTTT ATCGCTATCA TAAGGCAGCC 3061 CTTCTTTTGG AAGGCCTAAG TAGGATTCTA CAGGACCCTG CAGATATTGA AAATGTGCAT 3121 AAATATAAAT GTAGTATTGA GAGAAGACTG TCGGCGCTCT GCCATAGCAC CGCAACCGTG 3181 TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 3241 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 3301 CTTTATATAT CTTGTGGAAA GGACGAGGAA GCATCCTTCC GAGCCTACCA ACGCGTTAAG 3361 TCgacaatca acctctggat tacaaaattt gtgaaagatt