Construct: ORF TRCN0000471930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011841.1_s317c1
- Derived from:
- ccsbBroadEn_07859
- DNA Barcode:
- CGACTGTTATCACCCAATCAACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCB1 (23236)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23236 | PLCB1 | phospholipase C beta 1 | NM_015192.4 | 99.9% | 100% | 2988T>C |
| 2 | human | 23236 | PLCB1 | phospholipase C beta 1 | NM_182734.3 | 95.3% | 94.4% | (many diffs) |
| 3 | mouse | 18795 | Plcb1 | phospholipase C, beta 1 | NM_001145830.1 | 89.1% | 97.3% | (many diffs) |
| 4 | mouse | 18795 | Plcb1 | phospholipase C, beta 1 | NM_019677.2 | 85.6% | 92.6% | (many diffs) |
| 5 | mouse | 18795 | Plcb1 | phospholipase C, beta 1 | XM_006498930.2 | 84.6% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3714
- ORF length:
- 3648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggggctcaa cccggagtgc acgccttgca actcaagccc gtgtgcgtgt 121 ccgacagcct caagaagggc accaaattcg tcaagtggga tgatgactca actattgtta 181 ctccaattat tttgaggact gaccctcagg gatttttctt ttactggaca gatcaaaaca 241 aggagacaga gctactggat ctcagccttg tcaaagatgc cagatgtggg agacacgcca 301 aagctcccaa ggaccccaaa ttacgtgaac ttttggatgt ggggaacatc gggcgcctgg 361 agcagcgcat gatcacagtg gtgtatgggc ctgacctcgt gaacatctcc catttgaatc 421 tcgtggcttt ccaagaagaa gtggccaagg aatggacaaa tgaggttttc agtttggcaa 481 caaacctgct ggcccaaaac atgtccaggg atgcatttct ggaaaaagcc tatactaaac 541 ttaagctgca agtcactcca gaagggcgta ttcctctcaa aaacatatat cgcttgtttt 601 cagcagatcg gaagcgagtt gaaactgctt tagaggcttg tagtcttcca tcttcaagga 661 atgattcaat acctcaagaa gatttcactc cagaagtgta cagagttttc ctcaacaacc 721 tttgccctcg acctgaaatt gataacatct tttcagaatt tggtgcaaaa agcaaaccat 781 atcttaccgt tgatcagatg atggatttta tcaaccttaa gcagcgagat cctcggctta 841 atgaaatact ttatccacct ctaaaacaag agcaagtcca agtattgatt gagaagtatg 901 aacccaacaa cagcctcgcc agaaaaggac aaatatcagt ggatgggttc atgcgctatc 961 tgagtggaga agaaaacgga gtcgtttcac ctgagaaact ggatttgaat gaagacatgt 1021 ctcagcccct ttctcactat ttcattaatt cctcgcacaa cacctacctc acagctggcc 1081 aactggctgg aaactcctct gttgagatgt atcgccaagt gctcctgtct ggttgtcgct 1141 gtgtggagct ggactgctgg aagggacgga ctgcagaaga ggaacctgtc atcacccatg 1201 gcttcaccat gacaactgaa atatctttca aggaagtgat agaagcaatt gcggagtgtg 1261 catttaagac ttcacctttt ccaattctcc tttcgtttga gaaccatgtg gattccccaa 1321 agcagcaagc caagatggcg gagtactgcc gactgatctt tggggatgcc cttctcatgg 1381 agcccctgga aaaatatcca ctggaatctg gagttcctct tccaagccct atggatttaa 1441 tgtataaaat tttggtgaaa aataagaaga aatcacacaa gtcatcagaa ggaagcggca 1501 aaaagaagct ctcagaacaa gcctccaaca cctacagtga ctcctccagc atgttcgagc 1561 cctcatcccc aggagccgga gaagctgata cggaaagtga cgacgacgat gatgatgatg 1621 actgtaaaaa atcttcaatg gatgagggga ctgctggaag tgaggctatg gccacagaag 1681 aaatgtctaa tctggtgaac tatattcagc cagtcaagtt tgagtcattt gaaatttcaa 1741 aaaaaagaaa taaaagtttt gaaatgtctt ccttcgtgga aaccaaagga cttgaacaac 1801 tcaccaagtc tccagtggaa tttgtagaat ataacaaaat gcagcttagc aggatatatc 1861 caaaaggaac acgtgtggat tcatccaact atatgcctca gctcttctgg aatgcaggtt 1921 gtcagatggt ggcacttaat ttccagacaa tggacctggc tatgcaaata aatatgggga 1981 tgtatgaata caacgggaag agtggctaca gattgaagcc agagttcatg aggaggcctg 2041 acaagcattt tgatccattt actgaaggca tcgtagatgg gatagtggca aacactttgt 2101 ctgttaagat tatttcaggt cagtttcttt ctgataagaa agttgggact tacgtggaag 2161 tagatatgtt tggtttgcct gtggatacaa ggaggaaggc atttaagacc aaaacatccc 2221 aaggaaatgc tgtgaatcct gtctgggaag aagaacctat tgtgttcaaa aaggtggttc 2281 ttcctactct ggcctgtttg agaatagcag tttatgaaga aggaggtaaa ttcattggcc 2341 accgtatctt gccagtgcaa gccattcggc caggctatca ctatatctgt ctaaggaatg 2401 aaaggaacca gcctctgacg ctgcctgctg tctttgtcta catagaagtg aaagactatg 2461 tgccagacac atatgcagat gtcatcgaag ctttatcaaa cccaatccga tatgtgaacc 2521 tgatggaaca gagagctaag caattggctg ctttgacact ggaagatgaa gaagaagtaa 2581 agaaagaggc tgatcctgga gaaacaccat cagaggctcc aagtgaagcg agaacgactc 2641 cagcagaaaa tggggtgaat cacactacaa ccctgacacc caagccaccc tcccaggctc 2701 tccacagcca gccagctcca ggttctgtaa aggcacctgc caaaacagaa gatcttattc 2761 agagtgtctt aacagaagtg gaagcacaga ccatcgaaga actaaagcaa cagaaatcgt 2821 ttgtgaaact tcaaaagaaa cactacaaag aaatgaaaga cctggttaag agacaccaca 2881 agaaaaccac tgaccttatc aaagaacaca ctaccaagta taatgaaatt cagaatgact 2941 acttgagaag gagagccgct ttggaaaagt ccgccaaaaa ggacagtaag aaaaaatcgg 3001 aacccagcag ccctgatcat ggttcatcaa cgattgagca agacctcgct gccctggatg 3061 ctgaaatgac ccaaaagtta atagacttga aggacaaaca acagcagcag ctgcttaatc 3121 ttcggcaaga acagtattat agtgaaaaat accagaagcg agaacatatt aaactgctta 3181 ttcaaaagtt gacggatgtc gcagaagagt gtcagaacaa tcagttaaag aagctcaaag 3241 aaatctgtga gaaaGAAAAG AAAGAATTAA AGAAGAAAAT GGATAAAAAG AGGCAGGAGA 3301 AGATAACAGA AGCTAAATCC AAAGACAAAA GTCAGATGGA AGAGGAGAAG ACAGAGATGA 3361 TCCGGTCATA TATCCAGGAA GTGGTGCAGT ATATCAAGAG GCTAGAAGAA GCGCAAAGTA 3421 AACGGCAAGA AAAACTCGTA GAGAAACACA AGGAAATACG TCAGCAGATC CTGGATGAAA 3481 AGCCCAAGCT GCAGGTGGAG CTGGAGCAAG AATACCAAGA CAAATTCAAA AGACTGCCCC 3541 TCGAGATTTT GGAATTCGTG CAGGAAGCCA TGAAAGGAAA GATCAGTGAA GACAGCAATC 3601 ACGGTTCTGC CCCTCTCTCC CTGTCCTCAG ACCCTGGAAA AGTGAACCAC AAGACTCCCT 3661 CCAGTGAGGA GCTGGGAGGA GACATCCCAG GAAAAGAATT TGATACTCCT CTGTGCCCAA 3721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3841 TATCTTGTGG AAAGGACGAC GACTGTTATC ACCCAATCAA CCTACGCGTT AAGTCgacaa 3901 tcaacctctg gattacaaaa tttgtgaaag att