Construct: ORF TRCN0000472167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018172.1_s317c1
- Derived from:
- ccsbBroadEn_11406
- DNA Barcode:
- GTGGCTCGCATGTCCTGGGGGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP57 (9702)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9702 | CEP57 | centrosomal protein 57 | XM_006718946.3 | 58.4% | 55.2% | (many diffs) |
| 2 | human | 9702 | CEP57 | centrosomal protein 57 | NM_001243777.1 | 56.9% | 56.7% | 808_828del;832_1422del |
| 3 | human | 9702 | CEP57 | centrosomal protein 57 | NM_014679.5 | 53.9% | 53.8% | 808_811delAAAA;814_1500delinsG |
| 4 | human | 9702 | CEP57 | centrosomal protein 57 | NM_001243776.1 | 50.7% | 50.8% | (many diffs) |
| 5 | human | 9702 | CEP57 | centrosomal protein 57 | XM_017018592.1 | 50.7% | 50.8% | (many diffs) |
| 6 | human | 9702 | CEP57 | centrosomal protein 57 | XM_017018594.2 | 49.6% | 46.3% | (many diffs) |
| 7 | human | 9702 | CEP57 | centrosomal protein 57 | XM_017018593.2 | 48.7% | 48.5% | 502_503ins117;691_711del;715_1305del |
| 8 | human | 9702 | CEP57 | centrosomal protein 57 | NM_001363604.1 | 48.5% | 48.4% | 0_1ins81;727_730delAAAA;733_1419delinsG |
| 9 | human | 9702 | CEP57 | centrosomal protein 57 | XM_006718945.3 | 46.1% | 46% | 502_503ins117;691_694delAAAA;697_1383delinsG |
| 10 | human | 9702 | CEP57 | centrosomal protein 57 | XM_024448779.1 | 40.7% | 40.6% | (many diffs) |
| 11 | human | 9702 | CEP57 | centrosomal protein 57 | XR_001748050.2 | 22.6% | 1_228del;1039_3581del | |
| 12 | mouse | 74360 | Cep57 | centrosomal protein 57 | NM_001310721.1 | 50.4% | 51.4% | (many diffs) |
| 13 | mouse | 74360 | Cep57 | centrosomal protein 57 | NM_026665.4 | 47.8% | 48.8% | (many diffs) |
| 14 | mouse | 74360 | Cep57 | centrosomal protein 57 | XM_017313667.1 | 45.8% | 47.2% | (many diffs) |
| 15 | mouse | 74360 | Cep57 | centrosomal protein 57 | XR_001779042.1 | 26.2% | (many diffs) | |
| 16 | mouse | 74360 | Cep57 | centrosomal protein 57 | NR_037622.1 | 26% | (many diffs) | |
| 17 | mouse | 74360 | Cep57 | centrosomal protein 57 | XM_006510650.1 | 22.4% | 22.7% | (many diffs) |
| 18 | mouse | 74360 | Cep57 | centrosomal protein 57 | XM_006510649.1 | 21.1% | 21.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 876
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcgtct gtctctgcgg cttctggttc tcacttgtcg aacagctttg 121 ctgagccatc aaggtctaat ggaagcatgg ttcggcattc ttcatctcca tatgtagtat 181 atccttcgga taagcctttc cttaatagtg atctacgacg ctccccaagt aagcctacac 241 ttgcctatcc agaaagcaac agcagagcca tattttctgc tcttaagaat cttcaagata 301 agattcgacg cttggaactt gagaggattc aggcagaaga aagtgtgaaa accttgtcta 361 gagaaacaat tgaatataag aaagtactGG ATGAACAGAT ACAAGAAAGG GAGAATTCAA 421 AGAATGAGGA ATCAAAGCAC AATCAAGAAC TGACATCTCA GTTGTTAGCT GCAGAAAATA 481 AATGCAATCT ATTAGAAAAA CAATTGGAAT ACATGCGAAA TATGATAAAG CATGCCGAAA 541 TGGAGAGGAC ATCTGTCTTA GAGAAACAAG TTTCCCTAGA AAGAGAACGA CAACATGATC 601 AAACACATGT TCAGAGCCAA CTTGAAAAAT TGGATCTTCT TGAACAGGAG TATAACAAAC 661 TTACCACAAT GCAGGCCCTT GCAGAAAAAA AAATGCAAGA GTTGGAAGCA AAACTCCATG 721 AAGAAGAACA GGAAAGGAAA CGCATGCAAG CTAAGGCAGC TGAGTTGCAG ACTGGTCTAG 781 AAACAAATAG ACTTATCTTT GAAGATAAGG CAACTCCGTG TGTTCCCAAT GCAAGAAGAA 841 TTAAAAAAAA GAAGTCAAAA CCACCAGAAA AGGTGTGCCC AACTTTCTTG TACAAAGTGG 901 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 961 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1021 AGTGGCTCGC ATGTCCTGGG GGTCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1081 aatttgtgaa agatt