Construct: ORF TRCN0000472305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002733.1_s317c1
- Derived from:
- ccsbBroadEn_03136
- DNA Barcode:
- TCCACACCAAATTTCAATAGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNIP2 (30819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_173192.2 | 100% | 100% | |
| 2 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | XM_005269729.2 | 94.5% | 88.1% | (many diffs) |
| 3 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_173191.3 | 93.3% | 92.9% | 170_223del |
| 4 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_014591.5 | 88.4% | 88% | 170_268del |
| 5 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | XM_006717812.2 | 88.3% | 82.2% | (many diffs) |
| 6 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_173195.3 | 87.3% | 86.9% | 73_74ins96 |
| 7 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_173194.3 | 85.2% | 76.5% | (many diffs) |
| 8 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_173193.3 | 84.9% | 84.5% | 73_74ins96;199_219del |
| 9 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | XM_017016161.1 | 84.2% | 77.1% | (many diffs) |
| 10 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | XM_005269730.2 | 82.2% | 75.9% | (many diffs) |
| 11 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | XM_011539731.2 | 80.2% | 66.6% | (many diffs) |
| 12 | human | 30819 | KCNIP2 | potassium voltage-gated cha... | NM_173197.2 | 68.2% | 66% | (many diffs) |
| 13 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | NM_030716.3 | 92.1% | 97.2% | (many diffs) |
| 14 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | NM_145703.2 | 86% | 90.3% | (many diffs) |
| 15 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | XM_017318315.1 | 86% | 90.3% | (many diffs) |
| 16 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | XM_006527470.3 | 81.2% | 85.3% | (many diffs) |
| 17 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | NM_145704.2 | 79.8% | 84.5% | (many diffs) |
| 18 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | NM_001276358.1 | 76.6% | 74.6% | (many diffs) |
| 19 | mouse | 80906 | Kcnip2 | Kv channel-interacting prot... | XM_006527469.2 | 73.7% | 75.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 822
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gggccagggc cgcaaggaga gtttgtccga ttcccgagac ctggacggct 121 cctacgacca gctcacgggc caccctccag ggcccactaa aaaagcgctg aagcagcgat 181 tcctcaagct gctgccgtgc tgcgggcccc aagccctgcc ctcagtcagt gaaaacagcg 241 tggacgatga atttgaattg tccaccgtgt gtcaccggcc tgagggtctg gagcagctgc 301 aggagcaaac caaattcacg cgcaaggagt tgcaggTCCT GTACCGGGGC TTCAAGAACG 361 AATGTCCCAG CGGAATTGTC AATGAGGAGA ACTTCAAGCA GATTTACTCC CAGTTCTTTC 421 CTCAAGGAGA CTCCAGCACC TATGCCACTT TTCTCTTCAA TGCCTTTGAC ACCAACCATG 481 ATGGCTCGGT CAGTTTTGAG GACTTTGTGG CTGGTTTGTC CGTGATTCTT CGGGGAACTG 541 TAGATGACAG GCTTAATTGG GCCTTCAACC TGTATGACCT TAACAAGGAC GGCTGCATCA 601 CCAAGGAGGA AATGCTTGAC ATCATGAAGT CCATCTATGA CATGATGGGC AAGTACACGT 661 ACCCTGCACT CCGGGAGGAG GCCCCAAGGG AACACGTGGA GAGCTTCTTC CAGAAGATGG 721 ACAGAAACAA GGATGGTGTG GTGACCATTG AGGAATTCAT TGAGTCTTGT CAAAAGGATG 781 AGAACATCAT GAGGTCCATG CAGCTCTTTG ACAATGTCAT CTACCCAACT TTCTTGTACA 841 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 901 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 961 AGGACGATCC ACACCAAATT TCAATAGGCG CACGCGTTAA GTCgacaatc aacctctgga 1021 ttacaaaatt tgtgaaagat t