Construct: ORF TRCN0000472308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005472.1_s317c1
- Derived from:
- ccsbBroadEn_03948
- DNA Barcode:
- TTCGGCTATACTGTCGTCTGCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTMR14 (64419)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64419 | MTMR14 | myotubularin related protei... | NM_001077526.3 | 100% | 100% | |
| 2 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007038.2 | 95.8% | 95.8% | 819_820ins75 |
| 3 | human | 64419 | MTMR14 | myotubularin related protei... | NM_001077525.3 | 92% | 92% | 1614_1769del |
| 4 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007036.1 | 91.5% | 89.2% | (many diffs) |
| 5 | human | 64419 | MTMR14 | myotubularin related protei... | NM_022485.5 | 89.9% | 89.9% | 1432_1433ins180 |
| 6 | human | 64419 | MTMR14 | myotubularin related protei... | XM_005265400.2 | 88.1% | 88.1% | 819_820ins75;1539_1694del |
| 7 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007037.1 | 87.5% | 85.2% | (many diffs) |
| 8 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007042.2 | 85.7% | 85.7% | 819_820ins75;1357_1358ins180 |
| 9 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007034.1 | 84.5% | 82.4% | (many diffs) |
| 10 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007043.1 | 84.2% | 82.7% | 0_1ins133;26_27ins149 |
| 11 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007039.1 | 82% | 79.6% | (many diffs) |
| 12 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007035.1 | 80.8% | 78.7% | (many diffs) |
| 13 | human | 64419 | MTMR14 | myotubularin related protei... | XM_024453709.1 | 79.7% | 79.7% | 0_1ins363 |
| 14 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007040.1 | 78% | 75.7% | (many diffs) |
| 15 | human | 64419 | MTMR14 | myotubularin related protei... | XM_011534012.1 | 77.5% | 76.1% | 0_1ins133;26_27ins149;1332_1487del |
| 16 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007041.1 | 73.6% | 72.3% | (many diffs) |
| 17 | human | 64419 | MTMR14 | myotubularin related protei... | XM_011534013.1 | 73.3% | 73.3% | 0_1ins363;1251_1406del |
| 18 | human | 64419 | MTMR14 | myotubularin related protei... | XR_245152.2 | 72.5% | (many diffs) | |
| 19 | human | 64419 | MTMR14 | myotubularin related protei... | XR_001740233.2 | 69.3% | (many diffs) | |
| 20 | human | 64419 | MTMR14 | myotubularin related protei... | XR_001740231.1 | 66.6% | (many diffs) | |
| 21 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007045.2 | 64.2% | 64.2% | 0_1ins642 |
| 22 | human | 64419 | MTMR14 | myotubularin related protei... | XR_001740232.1 | 63.6% | (many diffs) | |
| 23 | human | 64419 | MTMR14 | myotubularin related protei... | XM_024453710.1 | 59% | 59% | 0_1ins642;972_1127del |
| 24 | human | 64419 | MTMR14 | myotubularin related protei... | XM_017007044.2 | 55.2% | 55.2% | 0_1ins642;177_178ins75;897_1052del |
| 25 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XM_006506823.3 | 89.2% | 90.4% | (many diffs) |
| 26 | mouse | 97287 | Mtmr14 | myotubularin related protei... | NM_026849.2 | 82.1% | 83.2% | (many diffs) |
| 27 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XM_006506824.3 | 77.4% | 70.2% | (many diffs) |
| 28 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XM_006506826.3 | 74.7% | 67.9% | (many diffs) |
| 29 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XR_377503.3 | 66.1% | (many diffs) | |
| 30 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XM_006506825.1 | 65.6% | 66.6% | (many diffs) |
| 31 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XR_001785185.1 | 62% | (many diffs) | |
| 32 | mouse | 97287 | Mtmr14 | myotubularin related protei... | XM_006506827.3 | 52.8% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1860
- ORF length:
- 1794
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcgctcgg gccgccgccg ccgctgcctc ggcggggtcc tcggcctctt 121 caggcaacca gccgcctcag gagctggggc ttggggagct gctggaggag ttctcccgga 181 ctcagtaccg ggccaaggat ggcagcggga ccggcggctc taaggttgag cgcattgaga 241 agagatgtct ggagctgttt ggccgagact actgtttcag cgtgattcca aacacgaatg 301 gggatatctg tggccactat ccccggcaca tcgtgttcct ggagtatgag agttctgaga 361 aggagaaaga cacgtttgag agtaccgtac aggtgagcaa gttgcaagac ctcatccacc 421 gcagcaagat ggcccggtgc agaggacggt ttgtctgccc agtaatcctg ttcaagggca 481 agcacatttg caggtcggcc acactggctg gatggggaga gctgtatgga cgctcaggct 541 acaactattt tttctcaggg ggtgcagatg atgcctgggc agatgtggag gacgtcacgg 601 aggaggactg tgctcttcga agtggtgaca cgcatctttt tgataaggtc agaggctatg 661 acatcaagct gcttcgatac ctgtcagtca aatacatctg tgacctgatg gtggagaaca 721 agaaggtgaa gtttggcatg aatgtaacct cctctgagaa ggtggacaaa gcccagcgct 781 atgccgactt cactctcctc tccatcccgt atccaggctg tgaatttttc aaggaatata 841 aagatcggga ttacatggca gaagggctca tatttaactg gaagcaggac tacgttgatg 901 ccccattgag catccccgac ttcctgactc actctctgaa cattgactgg agccagtatc 961 agtgttggga tctggtgcaa caaacacaaa actacctgaa gctgctgctt tccttagtta 1021 acagtgatga tgacagcggg ctgctggtac actgtatctc aggctgggat cggacccccc 1081 tcttcatctc cctcctgcgc ctttccttgt gggctgatgg gctcatccac acgtccctga 1141 agcccactga gatcctctac ctcactgtgg cctatgactg gttcctcttc gggcacatgt 1201 tggtagatcg gctcagcaaa ggggaggaga ttttcttctt ctgcttcaat tttttgaagc 1261 atattacctc cgaggagttc tctgctctga agacccagag gaggaagagt ttgccagccc 1321 gggatggagg cttcaccctg gaagacatct gcatgcTGAG ACGAAAGGAC CGTGGCAGCA 1381 CCACCAGCCT TGGCAGCGAC TTCTCCCTGG TCATGGAGAG TTCCCCAGGA GCCACTGGGA 1441 GCTTCACCTA TGAGGCCGTG GAGCTGGTCC CAGCAGGAGC GCCAACTCAG GCAGCTTGGA 1501 GGAAGAGCCA CTCATCCTCT CCACAGAGTG TCCTCTGGAA CCGGCCACAA CCCTCAGAGG 1561 ACCGCTTGCC TTCCCAGCAG GGGCTGGCGG AAGCCAGGTC TTCCAGCTCC TCTTCCTCAA 1621 ACCATTCTGA TAACTTTTTC AGGATGGGTA GCAGTCCCCT GGAGGTCCCC AAACCCAGGC 1681 TTGCAGCCCT GAGTGATCGA GAGACTCGGC TGCAGGAGGT GCGCTCAGCC TTCTTGGCTG 1741 CGTACAGCAG CACAGTGGGG CTTCGGGCAG TAGCCCCCAG TCCTTCCGGT GCCATCGGGG 1801 GCCTGCTGGA GCAATTTGCC CGTGGTGTTG GACTCCGGAG CATCAGCAGC AATGCCTTGT 1861 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1921 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1981 TTTATATATC TTGTGGAAAG GACGATTCGG CTATACTGTC GTCTGCGCAA CGCGTTAAGT 2041 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt