Transcript: Mouse XM_006506823.3

PREDICTED: Mus musculus myotubularin related protein 14 (Mtmr14), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mtmr14 (97287)
Length:
2338
CDS:
132..1922

Additional Resources:

NCBI RefSeq record:
XM_006506823.3
NBCI Gene record:
Mtmr14 (97287)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006506823.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000080739 CGCTCGGGATACAACTATTTA pLKO.1 594 CDS 100% 15.000 21.000 N Mtmr14 n/a
2 TRCN0000080740 CTCGGGATACAACTATTTATT pLKO.1 596 CDS 100% 15.000 21.000 N Mtmr14 n/a
3 TRCN0000288378 CTCGGGATACAACTATTTATT pLKO_005 596 CDS 100% 15.000 21.000 N Mtmr14 n/a
4 TRCN0000080742 TCCTGTTCAAAGGCAAGCATA pLKO.1 529 CDS 100% 4.950 6.930 N Mtmr14 n/a
5 TRCN0000307596 ATGAGGCAATTTCACACTAAT pLKO_005 2288 3UTR 100% 13.200 10.560 N Mtmr14 n/a
6 TRCN0000295589 TAAGGTGAGAGGCTATGATAT pLKO_005 707 CDS 100% 13.200 10.560 N Mtmr14 n/a
7 TRCN0000272864 ACTACTGTTTCAGCGTGATTC pLKO_005 331 CDS 100% 10.800 8.640 N MTMR14 n/a
8 TRCN0000080741 CCTGACTCAATCTCTGAACAT pLKO.1 986 CDS 100% 4.950 3.960 N Mtmr14 n/a
9 TRCN0000288304 CCTGACTCAATCTCTGAACAT pLKO_005 986 CDS 100% 4.950 3.960 N Mtmr14 n/a
10 TRCN0000080738 CAGGGCCTCATGGAGATTATA pLKO.1 2204 3UTR 100% 15.000 10.500 N Mtmr14 n/a
11 TRCN0000295636 TGTGGGCTGATGGGCTAATTC pLKO_005 1171 CDS 100% 13.200 9.240 N Mtmr14 n/a
12 TRCN0000002624 CAAGGAATATAAAGATCGGGA pLKO.1 893 CDS 100% 0.660 0.462 N MTMR14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006506823.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03948 pDONR223 99.5% 89.2% 90.4% None (many diffs) n/a
2 ccsbBroad304_03948 pLX_304 0% 89.2% 90.4% V5 (many diffs) n/a
3 TRCN0000472308 TTCGGCTATACTGTCGTCTGCGCA pLX_317 28.3% 89.2% 90.4% V5 (many diffs) n/a
4 ccsbBroadEn_03947 pDONR223 100% 82.1% 83.2% None (many diffs) n/a
5 ccsbBroad304_03947 pLX_304 0% 82.1% 83.2% V5 (many diffs) n/a
6 TRCN0000470894 AGCTTAAAGGCTTCACAACATCTT pLX_317 18.5% 82.1% 83.2% V5 (many diffs) n/a
7 ccsbBroadEn_15966 pDONR223 0% 62.8% 63.6% None (many diffs) n/a
8 ccsbBroad304_15966 pLX_304 0% 62.8% 63.6% V5 (many diffs) n/a
9 TRCN0000467037 CCCGTTGAGCGTCTTGCGCTGGGC pLX_317 23.5% 62.8% 63.6% V5 (many diffs) n/a
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