Construct: ORF TRCN0000472343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010879.1_s317c1
- Derived from:
- ccsbBroadEn_13922
- DNA Barcode:
- GATTGGCTTTTCCGTATGTTGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2K (5440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472343
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5440 | POLR2K | RNA polymerase II subunit K | NM_005034.4 | 99.4% | 98.2% | 173G>C |
| 2 | mouse | 17749 | Polr2k | polymerase (RNA) II (DNA di... | NM_023127.3 | 91.9% | 96.5% | (many diffs) |
| 3 | mouse | 102641086 | LOC102641086 | DNA-directed RNA polymerase... | XM_006536721.2 | 91.3% | 94.8% | (many diffs) |
| 4 | mouse | 100862456 | LOC100862456 | DNA-directed RNA polymerase... | XM_006537451.2 | 91.3% | 94.8% | (many diffs) |
| 5 | mouse | 17749 | Polr2k | polymerase (RNA) II (DNA di... | NM_001039368.2 | 53.8% | 56.5% | (many diffs) |
| 6 | mouse | 108167692 | Gm46136 | predicted gene, 46136 | XR_001779328.1 | 1.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 225
- ORF end:
- 399
- ORF length:
- 174
- Sequence:
-
1 GGGGGAGGGG TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG 61 GCCAGCTTGG CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG 121 GTTCATTCTC AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG 181 AGGCTAGCAT CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCATGGAC ACCCAGAAGG 241 ACGTTCAACC TCCAAAGCAG CAACCAATGA TATATATCTG TGGAGAGTGT CACACAGAAA 301 ATGAAATAAA ATCTAGGGAT CCAATCAGAT GCAGAGAATG TGGATACAGA ATAATGTACA 361 AGAAAAGGAC TAAAAGATTG GTCGTTTTTG ATGCTCCATG CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAGATTGG CTTTTCCGTA TGTTGTGTAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt