Construct: ORF TRCN0000472464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007076.1_s317c1
- Derived from:
- ccsbBroadEn_07185
- DNA Barcode:
- CGGGTAAACTCCGGGTTGATGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNAB1 (7881)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | NM_172159.3 | 99.8% | 100% | 99G>A;1044T>C |
| 2 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | NM_001308222.1 | 92.6% | 92.7% | 99G>A;513_514ins87;957T>C |
| 3 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | NM_172160.2 | 87.7% | 82.1% | (many diffs) |
| 4 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | NM_003471.3 | 87.5% | 81.7% | (many diffs) |
| 5 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | XM_017007171.2 | 83.4% | 80.8% | (many diffs) |
| 6 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | XM_011513115.3 | 83.3% | 82% | (many diffs) |
| 7 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | NM_001308217.1 | 80.9% | 75.2% | (many diffs) |
| 8 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | XM_011513116.3 | 79.7% | 76% | (many diffs) |
| 9 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | XM_011513117.3 | 73.4% | 73.5% | 0_1ins318;726T>C |
| 10 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | XM_017007173.2 | 73.4% | 73.5% | 0_1ins318;726T>C |
| 11 | human | 7881 | KCNAB1 | potassium voltage-gated cha... | XM_017007174.2 | 43.6% | 35.9% | (many diffs) |
| 12 | mouse | 16497 | Kcnab1 | potassium voltage-gated cha... | NM_010597.4 | 92.3% | 99.7% | (many diffs) |
| 13 | mouse | 16497 | Kcnab1 | potassium voltage-gated cha... | XM_006501057.1 | 81% | 80.4% | (many diffs) |
| 14 | mouse | 16497 | Kcnab1 | potassium voltage-gated cha... | XM_006501056.1 | 80.4% | 82.5% | (many diffs) |
| 15 | mouse | 16497 | Kcnab1 | potassium voltage-gated cha... | NM_001289450.1 | 66.7% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1269
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca agtctccata gcctgcacag agcacaattt gaagagtcgg aatggtgagg 121 accgacttct gagcaagcag agctccaccg cccccaatgt ggtaaacgca gcccgggcca 181 aattccgcac ggtcgctatc atcgcgcgca gcctggggac gttcacgcct cagcatcaca 241 tttctctcaa agagtccacc gcaaagcaga ctggcatgaa atataggaat cttggaaaat 301 caggactcag agtttcttgc ttgggtcttg gaacatgggt gacatttgga ggtcaaattt 361 cagatgaggt tgctgaacgg ctgatgacca tcgcctatga aagtggtgtt aacctctttg 421 atactgccga agtctatgct gctggaaagg ctgaagtgat tctggggagc atcatcaaga 481 agaaaggctg gaggaggtcc agtctggtca taacaaccaa actctactgg ggtggaaaag 541 ctgaaacaga aagagggctg tcaagaaagc atattattga aggattgaag ggctccctcc 601 agaggctgca gctcgagtat gtggatgtgg tctttgcaaa tcgaccggac agtaacactc 661 ccatggaaga aattgtccga gccatgacac atgtgataaa ccaaggcatg gcgatgtact 721 ggggcacctc gagatggagt gctatggaga tcatggaagc ctattctgta gcaagacagt 781 tcaatatgat cccaccggtc tgtgaacaag ctgagtacca tcttttccag agagagaaag 841 tggaggtcca gctgccagag ctctaccaca aaataggtgt tggcgcaatg acatggtctc 901 cacttgcctg tggaatcatc tcaggaaaat acggaaacgg ggtgccTGAA AGTTCCAGGG 961 CTTCACTGAA GTGCTACCAG TGGTTGAAAG AAAGAATTGT AAGTGAAGAA GGGAGAAAAC 1021 AGCAAAACAA GCTAAAAGAC CTTTCCCCAA TTGCGGAGCG TCTGGGATGC ACACTACCTC 1081 AGCTAGCTGT TGCGTGGTGC CTGAGAAACG AAGGTGTGAG TTCTGTGCTC CTGGGATCAT 1141 CCACTCCTGA ACAACTCATT GAAAACCTTG GTGCCATTCA GGTTCTCCCA AAGATGACAT 1201 CACATGTGGT AAATGAGATT GATAACATAC TGCGCAACAA GCCCTACAGC AAGAAGGACT 1261 ATAGATCATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGGGTA AACTCCGGGT TGATGAAAAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt