Construct: ORF TRCN0000472545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015222.1_s317c1
- Derived from:
- ccsbBroadEn_08580
- DNA Barcode:
- TCAGCCACCTGCCGAGTAATGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX32 (55760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55760 | DHX32 | DEAH-box helicase 32 (putat... | NM_018180.3 | 99.9% | 100% | 1530A>G |
| 2 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | NM_001286030.1 | 82.5% | 86.9% | (many diffs) |
| 3 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | NM_001286031.1 | 82.5% | 86.9% | (many diffs) |
| 4 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | NM_133941.2 | 82.5% | 86.9% | (many diffs) |
| 5 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | XM_006507137.1 | 82.5% | 86.9% | (many diffs) |
| 6 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | XM_006507138.1 | 82.5% | 86.9% | (many diffs) |
| 7 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | XM_017321903.1 | 80.6% | 84.9% | (many diffs) |
| 8 | mouse | 101437 | Dhx32 | DEAH (Asp-Glu-Ala-His) box ... | NM_001286032.1 | 68.6% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2295
- ORF length:
- 2229
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaagaaggg ctggagtgtc caaactcttc ctctgaaaaa cgctattttc 121 ctgaatccct ggattccagc gatggggatg aggaagaggt tttggcctgt gaggatttgg 181 aacttaaccc ctttgatgga ttgccatatt catcacgtta ttataaactt ctgaaagaaa 241 gagaagatct tcctatatgg aaagaaaaat actcctttat ggagaacctg cttcaaaatc 301 aaatcgtgat tgtttcagga gatgctaaat gtggtaagag cgctcaggtt cctcagtggt 361 gtgctgaata ttgtctttcc atccactacc agcacggggg cgtgatatgc acacaggtcc 421 acaagcagac tgtggtccag ctcgccctgc gggtggcgga tgaaatggat gttaacattg 481 gtcatgaggt tggctacgtg atccctttcg agaactgctg taccaacgaa acaatcctga 541 ggtattgtac tgatgatatg ctgcaaagag aaatgatgtc caatcctttt ttgggtagct 601 atggggtcat catcttagat gatattcatg aaagaagcat tgcaactgat gtgttacttg 661 gacttcttaa agatgtttta ctagcaagac cagaactgaa gctcataatt aactcctcac 721 ctcacctgat cagcaaactc aattcttatt atggaaacgt gcctgtcata gaagtgaaaa 781 ataaacaccc tgtggaggtt gtgtacctta gtgaggctca aaaggattct tttgagtcta 841 ttttacgcct tatctttgaa attcaccact cgggtgagaa aggtgacatt gtagtctttc 901 tggcctgtga acaagatatt gagaaagtct gtgaaactgt ctatcaagga tctaacctaa 961 acccagatct tggagaactg gtggttgttc ctttgtatcc aaaagagaaa tgttcattgt 1021 tcaagccact cgatgaaaca gaaaaaagat gccaagttta tcaaagaaga gtggtgttaa 1081 ctactagctc tggagagttt ttgatctgga gcaactcagt cagatttgtt atcgatgtgg 1141 gtgtggaaag aagaaaggtg tacaacccga gaataagagc aaactcgctc gtcatgcagc 1201 ccatcagcca gagccaggca gagatacgca agcagattct tggctcatct tcttcaggaa 1261 aatttttctg cctgtacact gaagaatttg cctccaaaga catgacgcca ctgaagccag 1321 cagaaatgca ggaagccaac ctaacaagca tggtgctttt tatgaagagg atagacattg 1381 cgggcctagg ccactgtgac ttcatgaaca gaccagcacc agaaagtttg atgcaggcat 1441 tggaagactt agattatctg gcagcactgg ataatgatgg aaatctttct gaatttggaa 1501 tcatcatgtc agagtttcct cttgatccac aactctcgaa gtctatctta gcgtcctgtg 1561 aatttgactg tgtagatgaa gtgctaacaa tcgcggccat ggtaacagct ccaaattgct 1621 tttcacatgt gccacatgga gctgaagagg ctgccttgac ttgttggaag acatttttac 1681 atcccgaagg agatcacttt accctcatca gcatttacaa ggcttaccaa gacacaactc 1741 tgaattctag cagtgagtac tgtgtggaaa agtggtgtcg tgattacttc ctcaactgtt 1801 cagcactcag aatggcagat gttattcgag ctgaacTCTT AGAAATTATC AAGCGAATCG 1861 AGCTTCCCTA TGCAGAACCT GCTTTTGGCT CCAAGGAAAA CACTCTAAAC ATAAAGAAAG 1921 CTCTTCTGTC CGGTTACTTT ATGCAGATTG CTCGGGATGT TGATGGATCA GGTAACTACT 1981 TAATGCTGAC ACATAAGCAG GTTGCTCAGC TGCATCCCCT GTCTGGTTAC TCAATCACCA 2041 AGAAGATGCC AGAGTGGGTC CTCTTCCATA AATTCAGCAT TTCTGAGAAC AACTACATCA 2101 GGATTACCTC AGAAATCTCT CCTGAACTAT TTATGCAGCT GGTACCACAA TACTATTTCA 2161 GTAATCTGCC TCCTAGTGAA AGTAAGGACA TTCTACAGCA AGTAGTGGAT CACCTATCCC 2221 CTGTGTCAAC AATGAATAAG GAACAGCAAA TGTGTGAGAC GTGCCCTGAA ACTGAACAGA 2281 GATGCACTCT CCAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCAGCCACCT GCCGAGTAAT 2461 GTTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt