Construct: ORF TRCN0000472587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002620.1_s317c1
- Derived from:
- ccsbBroadEn_01911
- DNA Barcode:
- AGGCTCCAGTCGACTTCTCCCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C6 (8361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472587
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 100% | 100% | |
| 2 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 84.8% | 100% | (many diffs) |
| 3 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 84.1% | 100% | (many diffs) |
| 4 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 84.1% | 100% | (many diffs) |
| 5 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 83.8% | 100% | (many diffs) |
| 6 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 83.8% | 100% | (many diffs) |
| 7 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 83.4% | 100% | (many diffs) |
| 8 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 83.4% | 100% | (many diffs) |
| 9 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 83.4% | 100% | (many diffs) |
| 10 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 82.5% | 100% | (many diffs) |
| 11 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 81.5% | 100% | (many diffs) |
| 12 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 80.9% | 100% | (many diffs) |
| 13 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 80.2% | 100% | (many diffs) |
| 14 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 80.2% | 100% | (many diffs) |
| 15 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 87% | 100% | (many diffs) |
| 16 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 86.4% | 100% | (many diffs) |
| 17 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 86.4% | 100% | (many diffs) |
| 18 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 86% | 100% | (many diffs) |
| 19 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 86% | 100% | (many diffs) |
| 20 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 85.7% | 100% | (many diffs) |
| 21 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 85.7% | 100% | (many diffs) |
| 22 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 85.1% | 100% | (many diffs) |
| 23 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 84.7% | 100% | (many diffs) |
| 24 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 84.5% | 100% | (many diffs) |
| 25 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 83.8% | 100% | (many diffs) |
| 26 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 83.1% | 100% | (many diffs) |
| 27 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 82.8% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGGTAG AGGCAAAGGT GGTAAAGGTT 121 TAGGAAAGGG AGGCGCCAAG CGCCATCGCA AAGTGCTGCG TGACAACATA CAGGGCATCA 181 CGAAGCCCGC CATCCGTCGC TTGGCCCGAC GCGGCGGCGT GAAACGCATT TCGGGCCTCA 241 TTTATGAGGA GACCCGCGGT GTTCTTAAGG TGTTCCTGGA GAATGTGATA CGGGACGCCG 301 TAACCTACAC GGAGCACGCC AAGCGTAAGA CAGTCACTGC AATGGATGTT GTCTACGCGC 361 TCAAGCGCCA GGGACGCACT CTGTACGGCT TTGGTGGCTG CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTAAATCTTG TGGAAAGGAC 541 GAAGGCTCCA GTCGACTTCT CCCACCACGC GTTAAGTCga caatcaacct ctggattaca 601 aaatttgtga aagatt