Construct: ORF TRCN0000472742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016797.1_s317c1
- Derived from:
- ccsbBroadEn_12486
- DNA Barcode:
- TCCCCAACCTCATGACTCCCGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEAF6 (64769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64769 | MEAF6 | MYST/Esa1 associated factor 6 | NM_001270875.2 | 100% | 100% | |
| 2 | human | 64769 | MEAF6 | MYST/Esa1 associated factor 6 | NM_001270876.2 | 95% | 91.8% | (many diffs) |
| 3 | human | 64769 | MEAF6 | MYST/Esa1 associated factor 6 | NM_022756.6 | 95% | 94.5% | 534_563del |
| 4 | human | 64769 | MEAF6 | MYST/Esa1 associated factor 6 | NR_073091.2 | 11.9% | 1_20del;552_609del;652_4793del | |
| 5 | human | 64769 | MEAF6 | MYST/Esa1 associated factor 6 | NR_073090.2 | 11.8% | 1_20del;552_639del;682_4823del | |
| 6 | human | 64769 | MEAF6 | MYST/Esa1 associated factor 6 | NR_073092.2 | 11.8% | (many diffs) | |
| 7 | mouse | 70088 | Meaf6 | MYST/Esa1-associated factor 6 | NM_001290701.1 | 90% | 98.9% | (many diffs) |
| 8 | mouse | 70088 | Meaf6 | MYST/Esa1-associated factor 6 | NM_027310.4 | 85.6% | 90.8% | (many diffs) |
| 9 | mouse | 70088 | Meaf6 | MYST/Esa1-associated factor 6 | XM_006503382.3 | 85.5% | 93.5% | (many diffs) |
| 10 | mouse | 70088 | Meaf6 | MYST/Esa1-associated factor 6 | XM_006503381.3 | 81% | 85.1% | (many diffs) |
| 11 | mouse | 70088 | Meaf6 | MYST/Esa1-associated factor 6 | XR_376354.3 | 35.8% | (many diffs) | |
| 12 | mouse | 70088 | Meaf6 | MYST/Esa1-associated factor 6 | XR_376353.3 | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gatgcacaac aaggcggcgc cgccgcagat cccGGACACC CGGCGGGAGC 121 TGGCGGAGCT CGTGAAGCGG AAGCAGGAGC TGGCGGAAAC ATTGGCAAAT TTGGAGCGAC 181 AGATCTATGC TTTTGAGGGA AGCTACCTGG AAGACACTCA GATGTATGGC AATATTATTC 241 GTGGCTGGGA TCGGTATCTG ACCAACCAAA AAAACTCCAA TAGCAAAAAT GATCGAAGGA 301 ACCGGAAGTT TAAGGAAGCT GAGCGGCTCT TCAGTAAATC CTCGGTTACC TCAGCAGCTG 361 CAGTAAGTGC ATTGGCAGGA GTTCAGGACC AGCTCATTGA AAAGAGGGAG CCAGGAAGTG 421 GGACGGAAAG TGACACTTCT CCAGACTTCC ACAATCAGGA AAATGAGCCC AGCCAGGAGG 481 ACCCTGAGGA TCTGGATGGA TCTGTGCAGG GAGTGAAACC TCAGAAGGCT GCTTCTTCTA 541 CTTCCTCAGG GAGTCACCAC AGCAGCCATA AAAAGCGAAA GAATAAAAAC CGGCACAGGA 601 TTGATCTGAA GTTAAACAAA AAACCACGAG CTGACTATTA CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGATCCCCA ACCTCATGAC TCCCGACGAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt