Transcript: Mouse XR_376353.3

PREDICTED: Mus musculus MYST/Esa1-associated factor 6 (Meaf6), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Meaf6 (70088)
Length:
1471
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_376353.3
NBCI Gene record:
Meaf6 (70088)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_376353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000176434 CAGACTTCCACAATCAGGAAA pLKO.1 422 3UTR 100% 4.950 3.960 N Meaf6 n/a
2 TRCN0000173195 CTGGGATCGGTATTTGACCAA pLKO.1 225 3UTR 100% 2.640 2.112 N Meaf6 n/a
3 TRCN0000276439 CTCAGATGTATGGCAATATTA pLKO_005 197 3UTR 100% 15.000 10.500 N MEAF6 n/a
4 TRCN0000437066 AGTAGAACCCAAGGTCCATTG pLKO_005 1079 3UTR 100% 6.000 4.200 N Meaf6 n/a
5 TRCN0000428666 ATATCTGCCATTCTCTTGTTT pLKO_005 1116 3UTR 100% 5.625 3.938 N Meaf6 n/a
6 TRCN0000175318 CTGGAAGACACTCAGATGTAT pLKO.1 187 3UTR 100% 5.625 3.938 N Meaf6 n/a
7 TRCN0000173690 CCTGGAAGACACTCAGATGTA pLKO.1 186 3UTR 100% 4.950 3.465 N Meaf6 n/a
8 TRCN0000173294 GCTCTTCAGCAAATCCTCAGT pLKO.1 306 3UTR 100% 2.640 1.848 N Meaf6 n/a
9 TRCN0000176384 CACACCTCTGATTCTGTGTTA pLKO.1 1051 3UTR 100% 4.950 2.970 N Meaf6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_376353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12486 pDONR223 100% 35.1% None (many diffs) n/a
2 ccsbBroad304_12486 pLX_304 0% 35.1% V5 (many diffs) n/a
3 TRCN0000472742 TCCCCAACCTCATGACTCCCGACG pLX_317 92% 35.1% V5 (many diffs) n/a
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