Construct: ORF TRCN0000472805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002933.1_s317c1
- Derived from:
- ccsbBroadEn_15941
- DNA Barcode:
- ATACAAACACTAAGAAAAATGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHTF2 (57157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127360.1 | 99.9% | 99.7% | 1037G>A |
| 2 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127359.1 | 98.7% | 98.5% | 102_113del;1049G>A |
| 3 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366083.1 | 76.4% | 73.1% | (many diffs) |
| 4 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366085.2 | 72.8% | 69.7% | (many diffs) |
| 5 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366082.2 | 63.5% | 60.9% | (many diffs) |
| 6 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366088.2 | 63.5% | 60.9% | (many diffs) |
| 7 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_017012459.2 | 51.2% | 49.1% | (many diffs) |
| 8 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516423.3 | 50.9% | 48.9% | (many diffs) |
| 9 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366081.1 | 49.9% | 48% | (many diffs) |
| 10 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366084.1 | 49.3% | 47.4% | (many diffs) |
| 11 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366087.1 | 49.3% | 47.4% | (many diffs) |
| 12 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516422.3 | 49.1% | 47.1% | (many diffs) |
| 13 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_006716063.4 | 49% | 47.1% | (many diffs) |
| 14 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127358.2 | 46.4% | 44.6% | (many diffs) |
| 15 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366086.1 | 46.4% | 44.6% | (many diffs) |
| 16 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_020432.5 | 46.4% | 44.6% | (many diffs) |
| 17 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127357.2 | 46.2% | 44.4% | (many diffs) |
| 18 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250508.5 | 46.2% | 44.4% | (many diffs) |
| 19 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250509.4 | 46.2% | 44.4% | (many diffs) |
| 20 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366089.1 | 44.2% | 42.5% | (many diffs) |
| 21 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516424.2 | 28.1% | 26.5% | (many diffs) |
| 22 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535804.3 | 84.5% | 85.5% | (many diffs) |
| 23 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535801.1 | 45.8% | 44% | (many diffs) |
| 24 | mouse | 68770 | Phtf2 | putative homeodomain transc... | NM_172992.3 | 41.3% | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccaaagtc acagatgcta tagtctggta tcaaaagaag attggagcat 121 atgatcaaca aatatgggaa aaatctgttg aacagagaga aatcaagggg ctaaggaata 181 aaccaaagaa aacagcacat gtgaaaccag acctcataga tgttgatctt gtaagagggt 241 ctgcatttgc aaaggcaaag cctgaaagtc cttggacttc tctgaccaga aagggaattg 301 ttcgagttgt atttttcccc tttttcttcc ggtggtggtt acaagtaaca tcaaaggtca 361 tctttttctg gcttcttgtc ctttatcttc ttcaagttgc tgcaatagta ttattctgct 421 ccacttctag cccacacagc atacctctga cagaggtgat tgggccgata tggctgatgc 481 tgctcctggg aactgtgcat tgccagattg tttccacaag aacacccaaa cctcctctaa 541 gtacaggggg taaaagaaga aggaaattaa gaaaagcagc ccatttggaa gtacataggg 601 aaggagatgg ttctagtacc acagataaca cacaagaggg agcagttcag aaccacggta 661 caagcacctc tcacagcgtt ggcactgtct tcagagatct ctggcatgct gctttctttt 721 tatcaggatc aaagaaagca aagaattcaa ttgataaatc aactgaaact gacaatggct 781 atgtatccct tgatgggaag aagactgtta aaagcggtga agatggaata caaaaccatg 841 aacctcagtg tgaaactatt cgaccagaag agacagcctg gaacacagga acactgagga 901 atggtcCTAG CAAAGATACC CAAAGGACAA TAACAAATGT CTCTGATGAA GTCTCCAGTG 961 AGGAAGGTCC TGAAACAGGA TACTCATTAC GTCGTCATGT GGACAGGACT TCTGAAGGTG 1021 TTCTTCGGAA TAGAAAGTCA CACCATTATA AGAAACATTA CCCTAATGAG GTATATACTT 1081 TGTCCTTAAG TGTATACATA CATATCTATT TTATATGTTA CTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGAATA CAAACACTAA GAAAAATGAC CACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t