Construct: ORF TRCN0000472939
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017105.1_s317c1
- Derived from:
- ccsbBroadEn_09520
- DNA Barcode:
- TGCCGCAGGGCTCACCCAATTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMID1 (129080)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472939
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129080 | EMID1 | EMI domain containing 1 | NM_133455.4 | 99.8% | 99.7% | 326C>G;1206A>G |
| 2 | human | 129080 | EMID1 | EMI domain containing 1 | NM_001267895.2 | 99.3% | 99.3% | 317_318insAGTTGC;320C>G;1200A>G |
| 3 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529869.3 | 96.1% | 96% | 326C>G;586_636del;1257A>G |
| 4 | human | 129080 | EMID1 | EMI domain containing 1 | XM_005261328.3 | 95.9% | 95% | (many diffs) |
| 5 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529868.3 | 92.4% | 91.5% | (many diffs) |
| 6 | human | 129080 | EMID1 | EMI domain containing 1 | XM_005261329.3 | 89.8% | 89% | (many diffs) |
| 7 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529870.3 | 88.7% | 81.3% | (many diffs) |
| 8 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529873.3 | 87.5% | 87.3% | (many diffs) |
| 9 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529871.3 | 86.5% | 85.7% | (many diffs) |
| 10 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529872.3 | 86.5% | 82.9% | (many diffs) |
| 11 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529874.2 | 78.5% | 77.5% | (many diffs) |
| 12 | human | 129080 | EMID1 | EMI domain containing 1 | XM_017028589.1 | 78.5% | 77.5% | (many diffs) |
| 13 | human | 129080 | EMID1 | EMI domain containing 1 | XR_937808.3 | 77.4% | (many diffs) | |
| 14 | human | 129080 | EMID1 | EMI domain containing 1 | XR_937809.3 | 74.8% | (many diffs) | |
| 15 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529875.2 | 59.2% | 58.3% | (many diffs) |
| 16 | human | 129080 | EMID1 | EMI domain containing 1 | XM_011529876.1 | 56.8% | 52.1% | (many diffs) |
| 17 | human | 129080 | EMID1 | EMI domain containing 1 | XR_937810.3 | 53.5% | (many diffs) | |
| 18 | mouse | 140703 | Emid1 | EMI domain containing 1 | NM_080595.2 | 84.3% | 80.2% | (many diffs) |
| 19 | mouse | 140703 | Emid1 | EMI domain containing 1 | XM_006514517.2 | 77.1% | 73.3% | (many diffs) |
| 20 | mouse | 140703 | Emid1 | EMI domain containing 1 | XR_380959.1 | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggcccgcgg gcttgggcgc tgctctgcct cgggctcctg ctcccgggag 121 gcggcgctgc gtggagcatc ggggcagctc cgttctccgg acgcaggaac tggtgctcct 181 atgtggtgac ccgcaccatc tcatgccatg tgcagaatgg cacctacctt cagcgagtgc 241 tgcagaactg cccctggccc atgagctgtc cggggagcag ctacagaact gtggtgagac 301 ccacatacaa ggtgatgtac aagatagtga ccgcccgtga gtggaggtgc tgccctgggc 361 actcaggagt gagctgcgag gaagttgcag gttcctctgc ctccttggag cccatgtggt 421 cgggcagtac catgcggcgg atggcgcttc ggcccacagc cttctcaggt tgtctcaact 481 gcagcaaagt gtcagagctg acagagcggc tgaaggtgct ggaggccaag atgaccatgc 541 tgactgtcat agagcagcca gtacctccaa caccagctac ccctgaggac cctgccccgc 601 tctggggtcc ccctcctgcc cagggcagcc ccggagatgg aggcctccag gaccaagtcg 661 gtgcttgggg gcttcccggg cccaccggcc ccaagggaga tgccggcagt cggggcccaa 721 tggggatgag aggcccacca ggtccacagg gccccccagg gagccctggc cgggctggag 781 ctgtgggcac ccctggagag aggggacctc ctgggccacc agggcctcct ggcccccctg 841 ggcccccagc ccctgttggg ccaccccatg cccggatctc ccagcatgga gacccattgc 901 tgtccaacac cttcactgag accaacaacc actggcccca gggacccact gggcctccag 961 gccctccagg gcccatgggt ccccctgggc ctcctggccc cacaggtgtc cctgggagtc 1021 ctggtcacat aggaccccca ggccccactg gacccaaagg aatctctggc cacccaggag 1081 agaagggcga gagaggactg cgtggggagc ctggccccca aggctctgct gggcagcggg 1141 gggaacctgg ccctaaggga gaccctggtg agaagagcca ctggggggag gggttgcacc 1201 agcTACGCGA GGCTTTGAAG ATTTTAGCTG AGAGGGTTTT AATCTTGGAA ACAATGATTG 1261 GGCTCTATGA GCCAGAGCTG GGGTCTGGGG CGGGCCCTGC CGGCACAGGC ACCCCCAGCC 1321 TCCTTCGGGG CAAGAGGGGC GGACATGCAA CCAACTACCG GATCGTGGCC CCCAGGAGCC 1381 GGGACGAGAG AGGCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCCGCAGGG CTCACCCAAT 1561 TTGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt