Construct: ORF TRCN0000472959

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004756.1_s317c1
Derived from:
ccsbBroadEn_06840
DNA Barcode:
AGAGCGGCCACCCTCAACTTCTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAP1GDS1 (5910)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472959
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100426.2 99.9% 99.8% 713A>G
2 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_021159.5 99.7% 99.6% 713A>G;1301_1302insAGC
3 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100427.2 99.7% 99.5% 3_4insGCA;710A>G
4 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454164.1 99.6% 99.3% 3_4insGCA;710A>G;1298_1299insAGC
5 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100428.2 91.8% 91.7% 364_365ins147;566A>G
6 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454165.1 91.7% 91.6% 364_365ins147;566A>G;1154_1155insAGC
7 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100429.2 91.7% 91.4% 3_4insGCA;361_362ins147;563A>G
8 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454166.1 91.5% 91.2% (many diffs)
9 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454167.1 86.6% 86.5% 0_1ins243;470A>G
10 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454168.1 86.6% 86.5% 0_1ins243;470A>G
11 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... NM_001100430.2 84.8% 84.5% 364_365ins276;437A>G
12 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454169.1 78.5% 78.4% 0_1ins243;121_122ins147;323A>G
13 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454170.1 78.5% 78.4% 0_1ins243;121_122ins147;323A>G
14 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454171.1 78.5% 78.4% 0_1ins243;121_122ins147;323A>G
15 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454172.1 78.3% 78.2% (many diffs)
16 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454173.1 78.3% 78.2% (many diffs)
17 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454174.1 70.5% 70.3% 0_1ins537;176A>G
18 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454175.1 70.5% 70.3% 0_1ins537;176A>G
19 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XM_024454176.1 70.5% 70.3% 0_1ins537;176A>G
20 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XR_002959742.1 67.6% (many diffs)
21 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XR_002959743.1 62% (many diffs)
22 human 5910 RAP1GDS1 Rap1 GTPase-GDP dissociatio... XR_002959744.1 61.9% (many diffs)
23 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... NM_001040690.2 89% 94.9% (many diffs)
24 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... NM_001286759.1 88.8% 94.7% (many diffs)
25 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... NM_145544.3 81.3% 87.5% (many diffs)
26 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... XM_017319566.1 81.1% 87.3% (many diffs)
27 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... XM_006501428.3 62.2% 68% (many diffs)
28 mouse 229877 Rap1gds1 RAP1, GTP-GDP dissociation ... XM_006501429.3 62.2% 68% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1893
ORF length:
1824
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcagataat ctcagtgata ccttgaagaa gctgaagata acagctgttg
121acaagactga ggatagttta gaaggatgct tggattgtct gcttcaagcc ctggctcaaa
181ataatacgga aacaagtgaa aaaatccaag caagtggaat acttcagctg tttgcaagtc
241tgttgactcc acagtcttcc tgcaaagcca aagtagctaa catcatagca gaagtagcca
301aaaatgagtt tatgcgaatt ccatgtgtgg atgctggatt gatttcacca ctggtgcagc
361tgctaaatag caaagaccag gaagtgctgc ttcaaacggg cagggctcta ggaaacatat
421gttacgatag ccatgagggc agaagtgcag ttgaccaagc aggtggtgca cagattgtaa
481ttgaccattt aaggtcactg tgcagtataa cagatcccgc caatgagaag ctcttgactg
541tcttttgtgg catgctgatg aactatagca atgagaatga ttcgcttcaa gctcagctta
601tcaatatggg tgttattcct accttagtga aattactggg catccactgc caaaatgcag
661ctcttacaga aatgtgtctt gttgcatttg gtaatttagc agaacttgag tcaagtaaag
721aacagtttgc cagtacaaac attgctgaag agctagtaaa actcttcaag aaacaaatag
781gacatgataa gagagaaatg atttttgaag ttcttgctcc attggcagaa aatgatgcta
841ttaaactaca gctggttgaa gcaggcctag tagagtgtct actagagatt gttcagcaaa
901aagtggatag tgacaaagaa gatgatatta ctgagctcaa aactggttca gatctcatgg
961ttttattact tcttggagat gaatccatgc agaagttatt tgaaggagga aaaggtagtg
1021tatttcaaag ggtactctct tggatcccat caaataacca ccagctacag cttgctggag
1081cattggcaat tgcaaatttt gccagaaatg atgcaaattg tattcatatg gtagacaatg
1141ggattgtaga aaaacttatg gatttactgg acagacatgt agaagatgga aatgtaacag
1201tacagcatgc agcactaagt gccctcagaa acctggccat tccagttata aataaagcaa
1261agatgttatc agctggggtc acagaggcag ttttgaaatt tcttaaatct gaaatgcctc
1321ctgttcagtt caaacttctg ggaacattaa gaatgttaat agatgcacaa gcagaagctg
1381ctgaacaatt gggaaagaat gttaagttag tggagcgttt ggtggaatgg tgtgaagcca
1441aagatcatgc tggtgtgatg ggggagtcaa acagactgct gtctgccctt atacgacaca
1501gtaaatcaaa agatgtaatt aaaaccattg tgcagagtgg tggcatcaag catctagtta
1561ccatggcaac tagtgaacat gtaataatgc agaatgaagc tcttgttgct ttggcattaa
1621tagcagcttt agaattgggc actgctgaga aagatctaga aagtgctaaa cttgtacaga
1681ttttacatag actgctagca gatgagagaa gtgctcctga aatcaaatat aattccatgg
1741tcctgatatg tgctcttatg ggatctgaat gtctacacaa ggaagtacag gatttggcTT
1801TTCTAGATGT CGTATCCAAA CTTCGCAGTC ATGAGAACAA AAGTGTTGCC CAGCAGGCCT
1861CTCTCACAGA GCAGAGACTT ACTGTGGAAA GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG
1921ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1981GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG
2041AGCGGCCACC CTCAACTTCT CTACGCGTTA AGTCgacaat caacctctgg attacaaaat
2101ttgtgaaaga tt

Download FASTA (ORF) (Full)