Construct: ORF TRCN0000473123
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011753.1_s317c1
- Derived from:
- ccsbBroadEn_15794
- DNA Barcode:
- AGGAAAGAGTACTACGAAAGAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF544 (27300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473123
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320782.1 | 84.7% | 72.8% | (many diffs) |
| 2 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320780.2 | 75% | 65.9% | (many diffs) |
| 3 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320783.2 | 73.6% | 46.4% | (many diffs) |
| 4 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320785.2 | 70.7% | 47.5% | (many diffs) |
| 5 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320786.2 | 70.7% | 47.5% | (many diffs) |
| 6 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320787.2 | 70.7% | 47.5% | (many diffs) |
| 7 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320788.1 | 66.2% | 50.4% | (many diffs) |
| 8 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320789.1 | 66.2% | 50.4% | (many diffs) |
| 9 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320791.1 | 66.2% | 50.4% | (many diffs) |
| 10 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320792.1 | 66.2% | 50.4% | (many diffs) |
| 11 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026599.1 | 53.1% | 47% | (many diffs) |
| 12 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320776.2 | 49.9% | 32.4% | (many diffs) |
| 13 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320777.2 | 47.2% | 32.9% | (many diffs) |
| 14 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026601.1 | 46.9% | 34.4% | (many diffs) |
| 15 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026602.1 | 46.2% | 34.5% | (many diffs) |
| 16 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026600.1 | 45.8% | 34.2% | (many diffs) |
| 17 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026604.1 | 45.4% | 33.3% | (many diffs) |
| 18 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320781.1 | 44.2% | 33.9% | (many diffs) |
| 19 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026603.1 | 44.2% | 33.9% | (many diffs) |
| 20 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_017026605.1 | 43.7% | 32.7% | 1_162del;321_322ins35;372_373ins73 |
| 21 | human | 27300 | ZNF544 | zinc finger protein 544 | XR_001753665.2 | 25% | (many diffs) | |
| 22 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320774.2 | 21.1% | 17.9% | (many diffs) |
| 23 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320767.1 | 14% | 11.2% | (many diffs) |
| 24 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_001320769.1 | 14% | 11.2% | (many diffs) |
| 25 | human | 27300 | ZNF544 | zinc finger protein 544 | NM_014480.3 | 14% | 11.2% | (many diffs) |
| 26 | human | 27300 | ZNF544 | zinc finger protein 544 | XM_011526738.2 | 13% | 10.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 408
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAAGCACG TTCTATGCTG GTTCCACCCC 121 AGGCATCTGT GTGCTTCGAG GATGTGGCTA TGGCATTCAC ACAGGAGGAG TGGGAACAGC 181 TGGACCTGGC CCAGAGGACA CTGTACCGAG AGGTGACACT GGAGACCTGG GAGCATATTG 241 TCTCCCTGGG GCTTTTCCTT TCCAAATCTG ATGTGATCTC TCAGCTGGAG CAAGAAGAGG 301 ACCTGTGCAG GGCAGAGCAG GAGGCCCCCC GAGGTACTTC AGGTCTTTCA CAAGTCACAG 361 GTGTAGTGGC TGATGGGAGC TTCAGGTTCC CGGTCTGGGA CTTCACATAC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAAGGAAAG AGTACTACGA AAGAAGGACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt